C57bl 6 strain 000664

Manufactured by Jackson ImmunoResearch

C57BL/6 (Strain 000664) is a widely used inbred mouse strain. It is a commonly utilized mouse model in biomedical research.

Automatically generated - may contain errors

Lab products found in correlation

Csf 1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

C57BL/6 (2462 citations) C57BL/6 strain (30 citations) Strain #000664 (10 citations) C57BL/6 (000664), (5 citations)

2 protocols using c57bl 6 strain 000664

Macrophage Differentiation and Fatty Acid Treatment

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 (Strain 000664) and Ppara^−/− knockout (Strain 008154) mice were obtained from Jackson Laboratory (Farmington, CT). Bone marrow was isolated from 8-10 week old C57BL/6 and Ppara^−/− knockout mice by the St. Jude Children’s Research Hospital Animal Resources Center, and macrophages were derived from the bone marrow using the method established by Toda et al. (2021) (link). Cells (4.2 x 10⁷) were seeded in 6-well tissue culture plates and grown in DMEM and 10% fetal bovine serum with 1 ng/ml CSF-1 (ThermoFisher) at 37°C 5% CO₂. On day 6, bone marrow-derived macrophages (BMDM) were washed with phosphate buffered saline and then changed to media containing 1% dimethyl sulfoxide (DMSO) ± fatty acid treatment and incubated 20-24 h. RAW 264.7 mouse macrophage cells (ATCC number TIB-71) were grown in DMEM plus 10% fetal bovine serum containing 1% DMSO ± fatty acid at 37°C 5% CO₂.

Radka C.D., Frank M.W., Simmons T.S., Johnson C.N., Rosch J.W, & Rock C.O. (2024). Staphylococcus aureus oleate hydratase produces ligands that activate host PPARα. Frontiers in Cellular and Infection Microbiology, 14, 1352810.

+ Open protocol

+ Expand

Transgenic Alzheimer's Disease Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 (strain 000664) and 5×FAD (strain 34840) mice were purchased from the Jackson Laboratories and maintained in our facilities. Mice bearing knock-in of human APOE2, APOE3, or APOE4 in the murine Apoe locus [B6.129P2-Apoe^{tm1(APOE*2)Mae} N9 (APOE2), B6.129P2-Apoe^{tm2(APOE*3)Mae} N8 (APOE3) and B6.129P2-Apoe^{tm3(APOE*4)Mae} N8 (APOE4)] were purchased from Taconic Biosciences and maintained in our facilities. Mice expressing each APOE allele were crossed with 5×FAD hemizygous mice to generate the final AD*APOE line. 5×FAD hemizygous*APOE homozygous mice were used in this study. Mice were weaned at the third postnatal week, genotyped in-house and kept on a 12 h/12 h light/dark cycle (lights on at 7:00) with access to food and water ad libitum.
Both female and male mice were used for experiments. Animal care and experimentation were according to NIH guidelines and were approved by the Institutional Animal Care and Use Committee at The Rockefeller University (protocols #20010 and #23007).

Millet A., Ledo J.H, & Tavazoie S. (2023). An Exhausted-Like Microglial Population Accumulates in Aged and APOE4 Genotype Alzheimer’s Brains. Immunity, 57(1), 153-170.e6.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!