Indoxyl sulfate was purchased from Biosynth (Staad, Switzerland). Phenyl sulfate and p-cresyl sulfate were synthesized at Eiweiss (Shizuoka, Japan). Indoleacetic acid was purchased from Tokyo chemical industry (Tokyo, Japan). Hydrogen peroxide and reduced glutathione were purchased from Wako Pure Chemical Industries (Osaka, Japan). Buthionine sulfoximine was purchased from Cayman Chemical (Ann Arbor, MI, USA). Meta-phosphoric acid was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Buthionine sulfoximine
Manufactured by Cayman Chemical
Sourced in United States
Buthionine sulfoximine is a chemical compound that inhibits the enzyme glutathione synthetase, which is involved in the synthesis of the antioxidant glutathione. It is used as a research tool in cell biology and biochemistry studies to manipulate cellular glutathione levels.
Automatically generated - may contain errors
Lab products found in correlation
Metaphosphoric acid, by Merck Group (1 mentions)
Hydrogen peroxide, by Fujifilm (1 mentions)
Reduced glutathione, by Fujifilm (1 mentions)
Indoxyl sulfate, by Biosynth (1 mentions)
Indoleacetic acid, by Tokyo Chemical Industry (1 mentions)
Fluoro carbonyl cyanide phenylhydrazone (fccp), by Cayman Chemical (1 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Antimycin a, by Cayman Chemical (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Hydrogen peroxide h2o2, by Merck Group (1 mentions)
2 protocols using buthionine sulfoximine
1
Synthesis and Procurement of Uremic Toxins
2
Redox Modulation of Mitochondrial Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Chemicals and small molecules used: carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) (Cayman Chemical Company), range 0.5-2.0 µM (stock 30 mM in 100% dimethylsulfoxide (DMSO)); Buthionine sulfoximine (BSO), range 0.01-10 mM ((stock solution 200 mM in Hanks balanced salt solution (HBSS)) (Cayman Chemical), Glutathione (Sigma) 2.0 mM and 20 mM (stock solution 300 in HBSS); N-acetyl cysteine (NAC) (Sigma), 5-30 mM (stock solution 500 mM in HBSS); antimycin A (Cayman Chemical) 2.0 µM (stock solution 10 mM in DMSO); hydrogen peroxide (H 2 O 2 ) (Sigma), 400 µM, diluted in HBSS before use.
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