Syn cel mir 39 spike in

Manufactured by Qiagen

Sourced in Japan

Syn-cel-miR-39 spike-in is a synthetic miRNA that is used as an internal control in miRNA quantification assays. It is designed to mimic the sequence and structure of the Caenorhabditis elegans miRNA cel-miR-39, and is spiked into samples to monitor the efficiency of RNA extraction, reverse transcription, and real-time PCR amplification.

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Lab products found in correlation

Taqman microrna assay, by Thermo Fisher Scientific (2 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (2 mentions) Rneasy kit, by Qiagen (2 mentions) Revertra ace qpcr rt kit, by Toyobo (2 mentions)

Close spelling variants of this product

Syn-cel-miR-39 spike in synthetic RNA Cel-miR-39 spike-in Syn-cel-miR-39 Cel-miR-39

2 protocols using syn cel mir 39 spike in

Quantifying mRNA and miRNA Expression

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Total RNA was extracted from cell lines using the RNeasy kit (QIAGEN, Tokyo, Japan). Complementary DNA was synthesized from 1 µg of total RNA using the ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan). The mRNA expression of genes was quantified using the TaqMan gene expression assay (Applied Biosystems, Foster City, CA). The expression of genes was normalized to that of B2M (beta-2-microglobulin).
We used miRNA-specific primers (TaqMan MicroRNA Assays, Applied Biosystems) for miRNA reverse transcription, and TaqMan gene expression assay for miRNA expression quantification. The expression data were normalized to RNU6B expression for cellular miRNAs and syn-cel-miR-39 spike-in (QIAGEN, Tokyo, Japan) expression for exosomal miRNAs.

Inoue T., Hayashi Y., Tsujii Y., Yoshii S., Sakatani A., Kimura K., Uema R., Kato M., Saiki H., Shinzaki S., Iijima H, & Takehara T. (2021). Suppression of autophagy promotes fibroblast activation in p53-deficient colorectal cancer cells. Scientific Reports, 11, 19524.

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Cell Proliferation Analysis via qRT-PCR

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We analyzed cell proliferation in 12-well plates (5 × 10 4 cells per well).
Quantitative real-time polymerase chain reaction (qRT-PCR)
Total RNA was extracted from cell lines using the RNeasy kit (QIAGEN, Tokyo, Japan). Complementary DNA was synthesized from 1 µg of total RNA using the ReverTra Ace™ qPCR RT Kit (Toyobo, Osaka, Japan). The mRNA expression of genes was quanti ed using the TaqMan gene expression assay (Applied Biosystems, Foster City, CA). The expression of genes was normalized to that of B2M (beta-2microglobulin).
We used miRNA-speci c primers (TaqMan MicroRNA Assays, Applied Biosystems) for miRNA reverse transcription, and TaqMan gene expression assay for miRNA expression quanti cation. The expression data were normalized to RNU6B expression for cellular miRNAs and syn-cel-miR-39 spike-in (Qiagen) expression for exosomal miRNAs.

Inoue T., Hayashi Y., Tsujii Y., Yoshii S., Sakatani A., Kimura K., Uema R., Katō M., Saiki H., Shinzaki S., Iijima H., & Takehara T. (2021). Suppression of Autophagy Promotes Fibroblast Activation in P53-deficient Colorectal Cancer Cells.

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