A unique set of 18 dual-labeled oligonucleotides and fluorescent probe was designed (Table 1). FLAP sequences (green color) were attached to the oligonucleotide sequence (blue color) at both ends and they are constituted the hybridization sites for probe (orange color) attachment (Fig. 1).
Next steps were performed based on the protocol described in the publication of Rensen (Rensen et al. 2022) . Tris-EDTA buffer (TE) (SigmaAldrich USA), pH = 8 was added to the synthesized oligonucleotides and the fluorescent probe (which were delivered in the form of a lyophilisate) in a volume specified for each sequence by the company (Genomed, Poznan, Poland) in order to obtain a solution with a concentration of 100µM. The fluorescent probe (100 µM) was frozen at -20 °C until the hybridization process was carried out. Each oligonucleotide was diluted in a separate tube to a concentration of 20 µM (20 µl of oligonucleotide was mixed with 80 µl of TE). 10 µl of each oligonucleotide (20 µM) of positive polarity was added to one test tube, and 10 µl of each oligonucleotide (20 µM) of negative polarity was added to the other. As in the case of the fluorescent probe, the oligonucleotides were stored at -20 °C until the hybridization process was carried out.
Next steps were performed based on the protocol described in the publication of Rensen (Rensen et al. 2022) . Tris-EDTA buffer (TE) (SigmaAldrich USA), pH = 8 was added to the synthesized oligonucleotides and the fluorescent probe (which were delivered in the form of a lyophilisate) in a volume specified for each sequence by the company (Genomed, Poznan, Poland) in order to obtain a solution with a concentration of 100µM. The fluorescent probe (100 µM) was frozen at -20 °C until the hybridization process was carried out. Each oligonucleotide was diluted in a separate tube to a concentration of 20 µM (20 µl of oligonucleotide was mixed with 80 µl of TE). 10 µl of each oligonucleotide (20 µM) of positive polarity was added to one test tube, and 10 µl of each oligonucleotide (20 µM) of negative polarity was added to the other. As in the case of the fluorescent probe, the oligonucleotides were stored at -20 °C until the hybridization process was carried out.