Proteins were extracted and analyzed for western blot by using the following antibodies: anti-GYS1 (Proteintech), anti-PYGL (Proteintech), anti-HIF-1a (Goodbio Company), anti-HPV11-E7 (Abcam, Cambridge, UK), and anti-b-actin (Abcam) at the recommended dilutions. The species source of the HPV11-E7 antibody is mouse and that of the b-actin antibody is rabbit.
Anti pygl
Manufactured by Proteintech
Sourced in United Kingdom
The Anti-PYGL product is a specific antibody that can be used to detect the presence and measure the levels of the PYGL protein in biological samples. PYGL is an enzyme involved in the breakdown of glycogen to glucose in the body. This antibody can be utilized in various research and analytical applications that require the identification and quantification of the PYGL protein.
Automatically generated - may contain errors
2 protocols using anti pygl
1
Western Blot Analysis of Cellular Proteins
2
Glycolytic Enzyme Profiling of Tissue
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Specimens were excised by standard surgical procedures and were cut into 4 mm sections. Serial sections were prepared and selected for staining with H&E (Baso, Taipei, Taiwan). Before staining, the sections were incubated with 0.1% BSA, then incubated overnight at 4 C with a target protein antibody. Biotinylated IgG was used as a secondary antibody, followed by streptavidin-conjugated horseradish peroxidase.
Target proteins were analyzed using the following antibodies: anti-GYS1, anti-PYGL, anti-CA9, anti-phosphogluconate dehydrogenase, anti-PRPS1, anti-HK2, anti-PFKP, anti-pyruvate dehydrogenase beta, anti-citrate synthase, anti-isocitrate dehydrogenase 3A, and anti-oxoglutarate dehydrogenase were purchased from Proteintech (Wuhan, China), anti-lactate dehydrogenase A and anti-HIF-1a were purchased from Goodbio Company (Wuhan, China). The species source of all the antibody was rabbit.
Sample sections were stained with periodic acid-Schiff (Baso) sets to detect glycogen levels according to the manufacturer's instructions.
Target proteins were analyzed using the following antibodies: anti-GYS1, anti-PYGL, anti-CA9, anti-phosphogluconate dehydrogenase, anti-PRPS1, anti-HK2, anti-PFKP, anti-pyruvate dehydrogenase beta, anti-citrate synthase, anti-isocitrate dehydrogenase 3A, and anti-oxoglutarate dehydrogenase were purchased from Proteintech (Wuhan, China), anti-lactate dehydrogenase A and anti-HIF-1a were purchased from Goodbio Company (Wuhan, China). The species source of all the antibody was rabbit.
Sample sections were stained with periodic acid-Schiff (Baso) sets to detect glycogen levels according to the manufacturer's instructions.
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