Rabbit polyclonal anti-Nanog, rabbit monoclonal anti-ACLY (EP704Y), and rabbit monoclonal phospho-ACLY (EP737Y) antibodies were purchased from Abcam (Cambridge, UK). Mouse monoclonal anti-Stat3 (124H6), rabbit monoclonal anti-phospho-Stat3 (Y705) (D3A7), rabbit polyclonal anti-ERK1/2, and rabbit polyclonal anti-phospho-ERK1/2 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-β-actin (AC-74) antibody was obtained from Sigma. Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare UK (Little Chalfont, UK).
Mouse monoclonal anti β actin ac 74 antibody
Manufactured by Merck Group
The Mouse monoclonal anti-β-actin (AC-74) antibody is a laboratory reagent used for the detection and quantification of the β-actin protein, a widely expressed and highly conserved cytoskeletal protein. This antibody can be used in various experimental techniques, such as Western blotting, immunocytochemistry, and immunohistochemistry, to identify and analyze the expression levels of β-actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit polyclonal anti nanog, by Abcam (1 mentions)
Rabbit polyclonal anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti rabbit igg secondary antibody, by GE Healthcare (1 mentions)
Rabbit polyclonal anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated anti mouse igg, by GE Healthcare (1 mentions)
Multi beads shocker, by Yasui Kikai (1 mentions)
2 protocols using mouse monoclonal anti β actin ac 74 antibody
1
Antibodies for Stem Cell Signaling
2
Protein Expression Analysis of TGF-β1 Signaling Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Skin or tumor samples were snap-frozen in liquid nitrogen, ground to a fine powder by Multi-Beads Shocker (Yasui Kikai, Inc.), lysed in SDS sample buffer and prepared as previously described [27 (link)]. Protein samples (30 μg) were subjected to SDS-polyacrylamide gel electrophoresis, and immunoblot analysis was performed as previously described [27 (link)]. The antibodies used in this study were: rabbit polyclonal anti-TGF-β1 (V) antibody (Santa Cruz Biotechnology), rabbit monoclonal anti-phospho-Smad2 (Ser465/467) (138D4) antibody (Cell Signaling Technology), rabbit monoclonal anti-Smad2 (D43B4) antibody (Cell Signaling Technology) and mouse monoclonal anti-β-actin (AC-74) antibody (Sigma-Aldrich, Saint Louis, MO). To confirm equal loading, membranes were reprobed with anti-β-actin antibody.
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