Recombinant human PNMT, NNMT, HNMT, COMT, GNMT, and GAMT were all acquired from Novus Biologicals (Centennial, CO). MTase Glo Kits, including the SAM cofactor we all purchased from Promega (Madison, WI). Norepinephrine, nicotinamide, glycine, guanidinoacetate, and histamine were all purchased from Sigma Aldrich (St. Louis, MO). Initial assay development, including KM determination, was performed in 384-well Lumitrac plates (White, flat bottom, medium binding, Cat# 781075, Greiner Bio-One, Monroe, NC). HTS assays were done in Aurora 1536 plates (white, flat bottom, medium binding Cat# AWK010000A, Aurora Microplates, Scottsdale, AZ). All data were analyzed and processed using in-house tools and TIBCO Spotfire 6.0.0 (Tibco Software Inc., Cambridge, MA. https://spotfire.tibco.com/ ). Data visualizations were generated in GraphPad Prism (GraphPad Software, San Diego, CA), TIBCO Spotfire, and Adobe Illustrator (Adobe, San Jose, CA).
Tibco spotfire
Manufactured by Adobe
TIBCO Spotfire is an advanced data analytics platform that enables users to explore, visualize, and analyze complex data from various sources. It provides a comprehensive set of tools for data preparation, visualization, and interactive reporting.
Automatically generated - may contain errors
Lab products found in correlation
Lumitrac plate, by Greiner (2 mentions)
Mtase glo kit, by Promega (2 mentions)
Glycine, by Merck Group (2 mentions)
Norepinephrine, by Merck Group (2 mentions)
Nicotinamide, by Merck Group (2 mentions)
Histamine, by Merck Group (2 mentions)
Guanidinoacetate, by Merck Group (2 mentions)
Recombinant human pnmt, by Novus Biologicals (2 mentions)
Tibco spotfire 6, by TIBCO Software (2 mentions)
2 protocols using tibco spotfire
1
Methyltransferase Enzyme Characterization
2
Kinetic Assays for Methyltransferase Enzymes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant human PNMT, NNMT, HNMT, COMT, GNMT, and GAMT were all acquired from Novus Biologicals (Centennial, CO). MTase Glo Kits, including the SAM cofactor we all purchased from Promega (Madison, WI). Norepinephrine, nicotinamide, glycine, guanidinoacetate, and histamine were all purchased from Sigma Aldrich (St. Louis, MO). Initial assay development, including K M determination, was performed in 384well Lumitrac plates (White, flat bottom, medium binding, Cat# 781075, Greiner Bio-One, Monroe, NC). HTS assays were done in Aurora 1536 plates (white, flat bottom, medium binding Cat# AWK010000A, Aurora Microplates, Scottsdale, AZ). All data were analyzed and processed using in-house tools and TIBCO Spotfire 6.0.0 (Tibco Software Inc., Cambridge, MA. https://spotfire.tibco.com/). Data visualizations were generated in GraphPad Prism (GraphPad Software, San Diego, CA), TIBCO Spotfire, and Adobe Illustrator (Adobe, San Jose, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!