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Cyanogen bromide

Manufactured by GE Healthcare
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Sourced in United Kingdom, United States

Cyanogen bromide is a chemical reagent used in various laboratory applications. It is a colorless, volatile liquid that is commonly used in the cleavage of proteins during biochemical analysis. This reagent is known for its ability to specifically cleave peptide bonds adjacent to methionine residues in proteins, making it a valuable tool for protein characterization and structural analysis.

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Lab products found in correlation

Sepharose 4b resin, by GE Healthcare (1 mentions) Activated sepharose 4b, by GE Healthcare (1 mentions)

Close spelling variants of this product

Cyanogen bromide-activated sepharose 4B (3 citations) Cyanogen bromide activated sepharose (2 citations) Cyanogen bromide–activated (2 citations) Cyanogen bromide beads (1 citations)

2 protocols using cyanogen bromide

1

Immobilization of Frutalin Lectin

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The purified frutalin was immobilized on a Sepharose 4B resin activated with cyanogen bromide (GE Healthcare, Buckinghamshire, UK). Lectin was incubated in the presence of its inhibitor sugar (0.2 M d-galactose) with the resin preactivated in an alkaline environment. All procedures followed the instructions provided by the manufacturer (GE Healthcare).
Lobo M.D., Moreno F.B., Souza G.H., Verde S.M., Moreira R.D, & Monteiro-Moreira A.C. (2017). Label-Free Proteome Analysis of Plasma from Patients with Breast Cancer: Stage-Specific Protein Expression. Frontiers in Oncology, 7, 14.
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2

Recombinant CEF Coupling to Sepharose 4B

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Recombinant CEF at a concentration of 2 mg/ml in PBS (pH 8.0) was mixed with Cyanogen bromide (CNBr)-activated sepharose 4B (GE Healthcare, Chicago, U.S.) overnight at 4°C. The remaining active sites on the beads were then blocked in 1 ml 100 mM Tris, pH 8.0 at RT for 2 h. After washing the beads three times with PBS (pH 8.0), the protein-coupled sepharose beads were stored in PBS (pH 8.0) with 0.025% NaN3 at 4°C.
Aghababa H., Ting Y.T., Pilapitiya D., Loh J.M., Young P.G, & Proft T. (2022). Complement evasion factor (CEF), a novel immune evasion factor of Streptococcus pyogenes. Virulence, 13(1), 225-240.
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