For neutrophil isolation CD45.1 mice were anesthetized using an isoflurane vaporizer and 1μg recombinant mouse CXCL1 (Peprotech) was delivered intranasally in PBS. Bronchoalveolar lavage (BAL) was collected after 3 hours, at which time >97% of recovered leukocytes were neutrophils. BAL cells were labeled with 5μM Carboxyfluorescein succinimidyl ester. After overnight culture ~60% of neutrophils were apoptotic as defined by annexin V staining, performed according to manufacturer’s instructions (BD Bioscience). These were co-incubated for 1 hour with peritoneal lavage cells after 47h in culture at an estimated ratio of 1:1 apoptotic neutrophils: MoMacs before collection for flow cytometry. Engulfing MoMacs were defined as CD64+ CFSE+ CD45.1- Ly6G- to exclude MoMacs that had bound but not internalized target cells.
Recombinant mouse cxcl1
Manufactured by Thermo Fisher Scientific
Recombinant mouse CXCL1 is a cytokine produced in E. coli cells. It functions as a chemoattractant for neutrophils.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant mouse cxcl1
1
Neutrophil Apoptosis Engulfment Assay
2
Transwell Neutrophil Migration Assay
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