Jc 1 mitochondria staining kit

Ginsenosides obtained from ChromaDex Inc (CA, USA). Poly(ethylene-co-vinyl alcohol) (EVAL), iron (III) chloride 6-hydrate (97%), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA) and JC-1 mitochondria staining kit For mitochondrial potential changes detection were from Sigma-Aldrich Co. (St. Louis, MO). 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) was from Wako Pure Chemical Industries, Ltd. (Osako, Japan). Iron (II) sulphate 7-hydrate (99.0%), hydrochloric acid and sodium hypochlorite were from Panreac (Barcelona, Spain). Acetonitrile and methanol high purity (>99.9%) for HPLC grade were from Fisher Scientific (NJ, U.S.A.) and Aencore Chemical PTY., LTD. (Surrey Hills, Australia), respectively. Dimethyl sulfoxide (DMSO, purity>99.9%) was ordered from J. T. Baker Chemical Co., (Phillipsburg, NJ). Lanosterol was obtained from Tokyo Chemical Industry Co., LTD (Japan). Gibco Dulbecco's modified Eagle medium and nutrient mixture F-12 (DMEM/F-12) were purchased from Life Technologies Corporation by Gibco company. Cobalt (II) chloride hexahydrate, (purity 98%) was from Alfa Aesar by Thermo Fisher Scientific. All chemicals were used as received unless otherwise mentioned.

Moderately differentiated human gastric cancer cell line SGC-7901 purchased from the Cell Bank of the Academy of Military Medical Science (Beijing, China) and human immortalized fetal gastric epithelial cell line GES-1 obtained from the Beijing Institute of Cancer Research (China)53 (link)–55 (link) were donated by State Key Laboratory of Cancer Biology, the Digestion Department of Xijing Hospital, and the Fourth Military Medical University (China). SGC-7901 and GES-1 cells were cultured in RPMI 1640 medium (HyClone Laboratories Inc., Logan, UT, USA) sup plemented with 10% fetal bovine serum (HyClone) and 1% penicillin/streptomycin in a humidified incubator at 37°C with 5% CO₂. Cetyltrimethylammonium bromide (CTAB), Pluronic F127, and JC-1 mitochondria staining kit were purchased from Sigma. Sodium borohydride, chloroauric acid (HAuCl₄), silver nitrate, and ascorbic acid were purchased from Aladdin, Cell Counting kit (CCK-8) from Dojindo, the trypan blue, Hoechst 33258/propidium iodide (PI) staining kits, Fluo-3/AM fluorescence probe from Beyotime Company, thiol PEG succinimidyl NHS (SH–PEG–NHS) from a local supplier, AlPcS₄ from Frontier Scientific, and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) cholesterol, and PEG2000-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) from Avanti Polar Lipids.