The primary antibody against total VASP (610448) was from BD biosciences (Franklin Lakes, NJ, USA). Anti-pCREB (9198S) and RIPA cell lysis buffer (9806) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-pHsp20 (58522) and anti-β-actin (58522) were purchased from Abcam (Cambridge MA, USA) and Sigma (St. Louis, MO, USA) respectively. Secondary antibodies IRDye 680RD or 800CW were from LI-COR (Lincoln, NE, USA). DGK inhibitor I (R59022), 3-Isobutyl-1-methylxanthine (IBMX), forskolin, histamine, H-89 dihydrochloride hydrate, indomethacin, and all other standard reagents were obtained from Sigma Life Sciences (St. Louis, MO, USA) unless otherwise mentioned. Protease and phosphatase inhibitors were from Bimake (Houston, TX, USA). Bisindolylmaleimide I (13298) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). U0126 (S1102) was procured from Selleckchem (Houston, TX, USA). All polyacrylamide gel casting, running, and transfer reagents and equipment were from Bio-Rad Laboratories (Hercules, CA, USA) or from previously identified sources (Michael et al., 2019 (link); Sharma et al., 2016 (link)).
Anti pcreb 9198s
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-pCREB (9198S) is a laboratory reagent produced by Cell Signaling Technology. It is a rabbit monoclonal antibody that specifically recognizes the phosphorylated form of the cAMP response element-binding protein (CREB).
Automatically generated - may contain errors
Lab products found in correlation
Protease and phosphatase inhibitor, by Selleck Chemicals (1 mentions)
Ripa cell lysis buffer, by Cell Signaling Technology (1 mentions)
Histamine, by Merck Group (1 mentions)
Bisindolylmaleimide 1 13298, by Cayman Chemical (1 mentions)
H 89 dihydrochloride hydrate, by Merck Group (1 mentions)
Irdye 680rd, by LI COR (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Ab151796, by Abcam (1 mentions)
Anti nf κb, by Proteintech (1 mentions)
Cyclopamine cyc, by Selleck Chemicals (1 mentions)
Anti shh, by Proteintech (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti bdnf, by Proteintech (1 mentions)
Anti gli1, by Abcam (1 mentions)
Anti creb, by Cell Signaling Technology (1 mentions)
Anti p nf κb, by Cell Signaling Technology (1 mentions)
Ab53715, by Abcam (1 mentions)
2 protocols using anti pcreb 9198s
1
Phosphorylation signaling in vascular smooth muscle cells
2
Investigating Shh/Gli-1 Signaling in Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The antibodies used for Western blot included anti-p-Akt (9271S, Cell Signaling Technology, USA), anti-Akt (9272S, Cell Signaling Technology, USA), anti-Shh (20697-1-AP, Proteintech Group, USA), anti-Gli-1 (ab151796, Abcam, USA), anti-Patch (ab53715, Abcam, USA), anti-NF-κB (10745-1-AP, Proteintech Group, USA), anti-p-NF-κB (3033S, Cell Signaling Technology, USA), anti-p-CREB (9198S, Cell Signaling Technology, USA), anti-CREB (9197S, Cell Signaling Technology, USA), anti-BDNF (17465-1-AP, Proteintech Group, USA), anti-neuroligin (ab186279, Abcam, USA), anti-neurexin (ab222806, Abcam, USA), and anti-β-actin (TA-09, Zhongshan Golden Bridge Biotechnology, China). The antibody for immunofluorescence was anti-Gli-1 (ab151796, Abcam, USA). PUR and cyclopamine (Cyc, a smoothened inhibitor) were purchased from Selleck Chemicals (Houston, TX, USA). Thread embolus was purchased from Guangzhou Jialing Biotechnology Co., Ltd. (L3800, Guangzhou, China). Annexin V-FITC/PI Double Labeling Apoptosis Detection Kit was purchased from BestBio Science (Shanghai, China). Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) kit was purchased from KeyGen Biotech (KGA7073, Beyotime, Shanghai, China).
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