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Cd235a glycophorin gly a pe cy7

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The CD235a/glycophorin (GLY)-A PE-Cy7 is a fluorochrome-conjugated antibody that binds to the CD235a/glycophorin-A antigen. CD235a/glycophorin-A is a sialoglycoprotein expressed on the surface of human erythrocytes. The PE-Cy7 conjugate allows for detection and analysis of CD235a/glycophorin-A-positive cells using flow cytometry.

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Lab products found in correlation

Cd19 pe hib19, by BD (2 mentions) Fitc 11e4b 7 6, by Beckman Coulter (2 mentions) Igm bv510 g20 127, by BD (2 mentions) Cd38 pe cy5 ls198 4 3, by Takara Bio (2 mentions) Cd15 apc hi98, by BD (2 mentions) Cd31 pecam pe wm59, by BD (2 mentions) Cd45 pe cy5 j33, by Beckman Coulter (2 mentions) May grunwald and giemsa stains, by Merck Group (2 mentions)

2 protocols using cd235a glycophorin gly a pe cy7

1

Multilineage Cell Identification by FACS

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Five thousand to 10,000 FACS-sorted erythroid cells (CD235a/glycophorin (GLY)-A PE-Cy7 or FITC (11E4B-7-6; Coulter)), plasmacytoid lymphocytes (CD19 PE (HIB19; BD), IgM BV510 (G20-127; BD), CD38 PE-Cy5 (LS198-4-3; Clontech)), neutrophils (CD15 APC (HI98; BD), CD31/PECAM PE (WM59; BD) and CD45 PE-Cy5 (J33; Coulter)) were cytospun onto slides (500 r.p.m. for 10 min), air dried, and stained with May-Grunwald and Giemsa stains (both from Sigma), washed with water, air dried, and mounted, followed by examination by light microscopy.
Sugimura R., Jha D.K., Han A., Soria-Valles C., da Rocha E.L., Lu Y.F., Goettel J.A., Serrao E., Rowe R.G., Malleshaiah M., Wong I., Sousa P., Zhu T.N., Ditadi A., Keller G., Engelman A.N., Snapper S.B., Doulatov S, & Daley G.Q. (2017). Haematopoietic stem and progenitor cells from human pluripotent stem cells. Nature, 545(7655), 432-438.
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2

Multilineage Cell Identification by FACS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five thousand to 10,000 FACS-sorted erythroid cells (CD235a/glycophorin (GLY)-A PE-Cy7 or FITC (11E4B-7-6; Coulter)), plasmacytoid lymphocytes (CD19 PE (HIB19; BD), IgM BV510 (G20-127; BD), CD38 PE-Cy5 (LS198-4-3; Clontech)), neutrophils (CD15 APC (HI98; BD), CD31/PECAM PE (WM59; BD) and CD45 PE-Cy5 (J33; Coulter)) were cytospun onto slides (500 r.p.m. for 10 min), air dried, and stained with May-Grunwald and Giemsa stains (both from Sigma), washed with water, air dried, and mounted, followed by examination by light microscopy.
Sugimura R., Jha D.K., Han A., Soria-Valles C., da Rocha E.L., Lu Y.F., Goettel J.A., Serrao E., Rowe R.G., Malleshaiah M., Wong I., Sousa P., Zhu T.N., Ditadi A., Keller G., Engelman A.N., Snapper S.B., Doulatov S, & Daley G.Q. (2017). Haematopoietic stem and progenitor cells from human pluripotent stem cells. Nature, 545(7655), 432-438.
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