The determination of proteins in the cell culture medium was performed using the following ELISA kits according to the manufacturers’ instructions: VIP (USCN Life Science Inc., Wuhan, China), IL-11 (RayBiotech, Peachtree Corners, GA, USA), HB-EGF (RayBiotech, Peachtree Corners, GA, USA), Platinum VEGF ELISA kit (eBioscience, San Diego, CA, USA), and SCF (R&D Systems, Minneapolis, MN, USA).
Hb egf
Manufactured by RayBiotech
Sourced in United States
HB-EGF is a laboratory product that functions as a recombinant human heparin-binding epidermal growth factor-like growth factor. It is a soluble protein that can be used in various cell culture and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Epidermal growth factor (egf), by RayBiotech (2 mentions)
Stem cell factor (scf), by R&D Systems (1 mentions)
Leptin, by RayBiotech (1 mentions)
Vegf a, by RayBiotech (1 mentions)
Hepatocyte growth factor (hgf), by RayBiotech (1 mentions)
Ang 2, by RayBiotech (1 mentions)
Angiogenin, by RayBiotech (1 mentions)
Tgf β1, by R&D Systems (1 mentions)
Vegf c, by R&D Systems (1 mentions)
Ang 1, by R&D Systems (1 mentions)
Basic fibroblast growth factor (bfgf), by RayBiotech (1 mentions)
Pdgf bb, by RayBiotech (1 mentions)
Tgf α, by RayBiotech (1 mentions)
3 protocols using hb egf
1
Quantification of Cell Culture Secretome
2
Profiling Angiogenic Factors in EC/VSMC
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Saphenous vein-derived ECs and VSMCs were separately cultured on 6-well plates and treated with TGFβ1 (0, 1, and 10 ng/ml) for 24 h. The resulting conditioned medium was harvested and used to determine the relative levels of AGFs by ELISA (VEGF-C, Ang-1, TGF-β1; R&D Systems Ltd) or Quantibody multiplex array (Angiogenin, Ang-2, EGF, bFGF, HB-EGF, HGF, Leptin, PDGF-BB, PlGF, VEGF-A; Raybiotech, Norcross, United States) according to the manufacturers’ instructions. Each experiment was performed on three separate occasions.
3
EGFR Ligand Quantification by ELISA
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Cells were seeded onto 6-well plates and incubated for 72 h before culture supernatants were collected for ELISA to determine ligand release. ELISA kits to detect the human 1 3
EGFR ligands EGF, HB-EGF, TGF-α, and amphiregulin were purchased from Ray Biotech, Inc..
EGFR ligands EGF, HB-EGF, TGF-α, and amphiregulin were purchased from Ray Biotech, Inc..
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