Polarstar omega

Manufactured by BMG Labtech

Sourced in Germany, United Kingdom, United States, Australia, France

The POLARstar Omega is a multimode microplate reader designed for a wide range of detection methods, including absorbance, fluorescence, and luminescence. It offers high sensitivity and flexibility to support various life science applications.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Merck Group (15 mentions) Passive lysis buffer (plb), by Promega (10 mentions) Omega mars data analysis software package, by BMG Labtech (8 mentions) Dimethyl sulfoxide (dmso), by Merck Group (7 mentions) Dual luciferase assay kit, by Promega (6 mentions) Celltiter 96 aqueous one solution cell proliferation assay, by Promega (4 mentions) Mueller hinton broth (mhb), by Merck Group (4 mentions) Sytox green, by Thermo Fisher Scientific (4 mentions) Triton x 100, by Merck Group (4 mentions) Celltiter glo luminescent cell viability assay kit, by Promega (4 mentions) Ripa buffer, by Thermo Fisher Scientific (4 mentions) Prism 6, by GraphPad (4 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) White wall, by Thermo Fisher Scientific (3 mentions) Adhesive plate sealers, by Avantor (3 mentions) Microtiter plate, by Corning (3 mentions) Toxilight bioassay, by Lonza (3 mentions) Pdelight hts camp phosphodiesterase assay kit, by Lonza (3 mentions) Cellular ros detection assay kit, by Abcam (3 mentions) Fluostar optima, by BMG Labtech (3 mentions)

Close spelling variants of this product

POLARstar Omega microplate reader (110 citations) POLARstar Omega spectrophotometer (23 citations) POLARstar Omega system (2 citations) POLARstar Omega reader (5 citations) POLARstar Omega fluorimeter (3 citations) POLARstar Omega plate (16 citations) POLARStar Omega microplate spectrophotometer (2 citations) POLARstar Omega Instrument (3 citations) POLARstar Omega Multi-Mode Microplate Reader with Fluorescent Polarization (2 citations) POLARstar Omega plater reader (2 citations)

453 protocols using polarstar omega

Endometrial Cell MTT Viability Assay

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Cell viability was assessed using the mitochondria-dependent reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Merck), as described and validated previously for use in endometrial cells challenged with pyolysin.⁹ Briefly, cells were incubated for 2 h in 250 μl/well serum-free culture medium containing 1 mg/ml MTT. The medium was then discarded, and cells were lysed with 300 μl DMSO (Merck). Optical density (OD₅₇₀) was measured using a POLARstar Omega microplate reader (POLARstar Omega; BMG Labtech, Aylesbury, UK).

Ormsby T.J., Owens S.E., Horlock A.D., Davies D., Griffiths W.J., Wang Y., Cronin J.G., Bromfield J.J, & Sheldon I.M. (2021). Oxysterols protect bovine endometrial cells against pore-forming toxins from pathogenic bacteria. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(10), e21889.

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Endometrial Cell MTT Viability Assay

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Mitochondrial Viability Assay with MTT

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The viability of cells was assessed by the mitochondria-dependent reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Merck) as described previously (21 (link)). Briefly, cells were incubated in 250 µl/well of serum-free medium containing 1 mg/ml MTT for 2 h, the medium was then discarded, and cells were lysed with 300 µl of dimethyl sulfoxide (Merck). Optical density (OD₅₇₀) was measured using a POLARstar Omega micro plate reader (POLARstar Omega; BMG, Labtech, Ortenberg, Germany).

Ormsby T.J., Owens S.E., Clement L., Mills T.J., Cronin J.G., Bromfield J.J, & Sheldon I.M. (2022). Oxysterols Protect Epithelial Cells Against Pore-Forming Toxins. Frontiers in Immunology, 13, 815775.

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Cell Viability Assays with MTT and DNA Quantification

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The viability of cells was assessed by the mitochondria-dependent reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Merck)^{10 (link),56 (link)}. Cells were incubated in 250 µl/well serum-free culture media containing 1 mg/ml MTT for 2 h. The medium was then discarded, and cells were lysed with 300 µl/well dimethyl sulfoxide (Merck). Optical density (OD₅₇₀) was measured using a POLARstar Omega microplate reader (POLARstar Omega; BMG, Labtech, Offenburg, Germany). The viability of dermal fibroblasts was assessed by quantifying cellular DNA using the CyQUANT Cell Proliferation Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

Ormsby T.J., Owens S.E., Turner M.L., Cronin J.G., Bromfield J.J, & Sheldon I.M. (2023). Glucocorticoids increase tissue cell protection against pore-forming toxins from pathogenic bacteria. Communications Biology, 6, 186.

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Quantifying Cell Viability with CellTiter-Glo and Crystal Violet

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Cell viability was assessed by CellTiter-Glo or crystal violet staining. For CellTiter-Glo, 96-well plates were removed from incubator and placed at room temperature for 30 minutes to equilibrate. Room temperature CellTiter-Glo reagent was added and cells were shaken for 2 minutes. Plates were then incubated for 10 min at room temperature. Luminescence was measured using a POLARStar Omega plate reader (BMG LabTech, Ortenberg, Germany; Em Filter – empty; Gain = 3600, orbital averaging ON, diameter = 5, cycles = 6) or a Synergy 2 Microplate Reader (Biotek, Winooski, VT; area scan; integration time = 0.50 s).
For crystal violet staining, AML12 cells were rinsed in PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes. Two water washes were performed and cells were stained with 0.25% crystal violet in 10% methanol for 20 minutes. Finally, three water washes were performed and plates were allowed to dry at room temperature for at least 24 hours. Images were captured with a custom digital photography set-up on a Canon 5D, MkI with a Sigma 150–600 mm lens. To quantify crystal violet staining, dye was dissolved in 10% acetic acid (300 μl per well). An aliquot was removed to a clear 96-well plate and A590 absorbance was measured using a POLARStar Omega plate reader (BMG LabTech). Signal was kept in the linear range by 1:2 – 1:4 dilution with 10% acetic acid where necessary.

Dinh T.A., Sritharan R., Smith F.D., Francisco A.B., Ma R.K., Bunaciu R.P., Kanke M., Danko C.G., Massa A.P., Scott J.D, & Sethupathy P. (2020). Hotspots of Aberrant Enhancer Activity in Fibrolamellar Carcinoma Reveal Candidate Oncogenic Pathways and Therapeutic Vulnerabilities. Cell reports, 31(2), 107509.

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Cytotoxicity Evaluation of p53 in Neuronal Cells

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Primary neurons derived from C57BL/6 and Tau KO mice were cultured and treated for measuring cytotoxicity using LDH release assay (Cytotoxicity Detection Kit PLUS-LDH, #04744926001, Roche) following the manufacturers’ instructions as previously described [74 (link), 102 (link)]. Briefly, primary neurons were maintained in Neuroblast medium supplement with B-27. Primary neurons (3 × 10⁵ cells /well) derived from C57BL/6 (n = 2) and tau KO (n = 1) were treated for 24 h with 0.5 μM or 1.0 μM p53 monomer, p53 oligomer, p53 fibrils, and p53 mixtures (each treatment performed in triplicate) and assessed by LDH release. OD was measured at 490 nm with POLARstar OMEGA plate reader (BMG Labtechnologies).

Farmer K.M., Ghag G., Puangmalai N., Montalbano M., Bhatt N, & Kayed R. (2020). P53 aggregation, interactions with tau, and impaired DNA damage response in Alzheimer’s disease. Acta Neuropathologica Communications, 8, 132.

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Fluorescence-based Protein Aggregation Assay

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Samples were prepared by adding 0.6 μg of protein and 248 μl of 10 mM bis-ANS, in 100 mM glycine–NaOH buffer (pH 7.4), in a clear bottom 96-well black plate. Each experiment was performed in triplicate. The excitation and emission wavelengths used to measure fluorescence intensity were 380 nm and 520 nm, respectively.
For ThT assay, samples were prepared using 0.6 μg of protein and 248 μl of 5 mM ThT, 50 mM glycine–NaOH buffer (pH 8.5). Each experiment was performed in triplicate. The fluorescence intensity was measured at an excitation wavelength of 440 nm and an emission wavelength of 490 nm using a POLARstar OMEGA plate reader (BMG Labtechnologies).
Fluorescence spectra of Bis-ANS and ThT + vehicle were used to correct for the background fluorescence.

Bhatt N., Puangmalai N., Sengupta U., Jerez C., Kidd M., Gandhi S, & Kayed R. (2024). C9orf72-associated dipeptide protein repeats form A11-positive oligomers in amyotrophic lateral sclerosis and frontotemporal dementia. The Journal of Biological Chemistry, 300(2), 105628.

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Fluorescence-based Aggregation Assays for Alpha-Synuclein and Tau

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Three microliters of either α-Syn or tau aggregates (0.5 and 0.6 μg/ μl, respectively) and 247 μl of 10 μM bis-ANS (4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt, Invitrogen) prepared in 100 mM glycine-NaOH buffer (pH 7.4) were added to the wells of 96-well clear-bottomed black plates. Each condition was performed in triplicate. The fluorescence intensity was measured at λ-emission 520 nm upon λ-excitation 380 nm. For Thioflavin T (ThT) assay, 3 μl of protein (0.5 and 0.6 μg/μl, respectively) and 247 μl of 20 μM ThT prepared in 50 mM glycine-NaOH buffer (pH 8.5) were added in triplicates to the wells. Fluorescence intensity was read at λ-emission 490 nm following excitation at 440 nm using a POLARstar OMEGA plate reader (BMG Lab technologies). Each condition for this assay was performed in triplicate.

Sengupta U., Puangmalai N., Bhatt N., Garcia S., Zhao Y, & Kayed R. (2020). Polymorphic α-Synuclein Strains Modified by Dopamine and Docosahexaenoic Acid Interact Differentially with Tau Protein. Molecular Neurobiology, 57(6), 2741-2765.

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Nanoparticle-Mediated Radiosensitization Assay

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Capan-1 cells (10,000 cells/well) were seeded in 96-well plates and grown for 24 h. The cells were then incubated with different concentrations of nanoparticles for 30 min, and washed with PBS to remove nanoparticles that were not internalized by the cells. Afterwards, cells were incubated with 10 μM dihydrorhodamine 123 (DHR123) for 3 h. Prior to irradiation, cells were washed with PBS to remove excess DHR. Irradiations were performed with a single fraction of 4 Gy irradiation (10 cm depth, 15 × 15 cm² field size) with 6 MV or 6 MV-FFF radiation beams. The fluorescence signal was measured 3 h post-irradiation using a plate reader (POLARstar omega, BMG LABTECH) with an excitation wavelength of 480 nm and an emission wavelength of 520 nm.

Detappe A., Kunjachan S., Drané P., Kotb S., Myronakis M., Biancur D.E., Ireland T., Wagar M., Lux F., Tillement O, & Berbeco R. (2016). Key clinical beam parameters for nanoparticle-mediated radiation dose amplification. Scientific Reports, 6, 34040.

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PolyA Encapsulation Efficiency Assay

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PolyA encapsulation efficiency (EE%) was measured using Quant-iT™ RiboGreen™ RNA Assay Kit (Invitrogen™). Briefly, 100 µL of the diluted fluorescent dye was added to 100 µL liposomes and incubated in absence of light for 5 min. Non-encapsulated PolyA was quantified by measuring fluorescence (λem= 480 nm, λex= 520nm) using a fluorimeter (Polarstar Omega, BMG Labtech). A linear calibration curve was obtained (R 2 =0.997) from 0 -1000 ng/mL PolyA with LOD and LOQ of 75 and 228 ng/mL respectively.

Webb C., Forbes N., Roces C.B., Anderluzzi G., Lou G., Abraham S., Ingalls L., Marshall K., Leaver T.J., Watts J.A., Aylott J.W, & Perrie Y. (2020). Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes. International journal of pharmaceutics, 582.

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