Eca9706

Human ESCC cell lines Kyse150, Eca109, Eca9706 and TE‐1 were purchased from the cell bank of the Chinese Academy of Sciences and cultured in RPMI‑1640 medium (Gibco; Thermo Fisher Scientific, Inc, Waltham, MA, USA) containing 10% foetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, Inc), streptomycin (100 mg/ml) and penicillin (100 U/ml). All cells were cultured in a humidified incubator at 37℃ with 5% CO₂.

Human esophageal cancer cell lines (Eca109, Eca9706, KYSE30, KYSE510, KYSE520, KYSE140 and KYSE150) were obtained either from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) or purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany) and cultured at 37°C with 5% CO2 in Dulbecco’s Modified Essential Medium (DMEM) medium (Hyclone, Logan, Utah, USA) supplemented with 10% fetal bovine serum (FBS) (Gibico, Carlsbad, California, USA). Human esophageal epithelial squamous cell NE1 was a kind gift from Dr. Yang XZ at Cancer Hospital and Institute of Guangzhou Medical University (Guangzhou, China) were cultured in a 1:1 mixture of defined keratinocyte serum free medium with growth supplements and EpiLife medium with 60 μM Calcium (Invitrogen,Carlsbad, California, USA). BAY 11-7082 and TNFα were purchased from Selleck (Huston, Texas, USA) and dissolved according to the manufacturer instructions. The fluorescent stain 4',6-diamidino-2-phenylindole (DAPI) and second antibodies used for immunofluorescence were purchased from Invitrogen (Carlsbad, California, USA).

The human esophageal cancer cell lines (Eca109, Eca9706, TE-1, TE-10, TE-11, KYSE140, and KYSE150) were obtained from the Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium (HyClone, USA) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco-BRL), 100 μg/ml streptomycin and 100 units/ml penicillin G (Beyotime Biotechnology, Shanghai, China) in 5% CO₂ and humidified atmosphere at 37 °C. Cells at the third generation of the exponentially growing state were used for all experiments.