HCC specimens and the matched adjacent non-malignant tissues (3–5 cm distal to the edge of tumor) were collected from patients with HCC being treated at the First Affiliated Hospital of Xi’an Jiaotong University after obtaining informed consent. None of patients received any chemotherapy or radiotherapy treatments before surgery. HCC diagnosis was confirmed by histopathology. The consents used in the study were reviewed and approved by the the Hospital’s Ethics Committee. The human HCC cell lines (Hep3B, HepG2, SMCC-7721, MHCC-97L, MHCC-97H, Huh7 and the immortalized human hepatic cell line LO2 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China).All cells are epithelial-like morphology, growth of adherent. All cells were stored in liquid nitrogen and cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA), 100 μg/mL streptomycin and 100 U/mL penicillin (Sigma, USA). All cells were maintained at 37 °C in 5% CO2 humidified incubator.
Smcc 7721
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The SMCC-7721 is a specialized laboratory equipment designed for the collection, preservation, and storage of authenticated cell cultures. It is a critical tool for researchers and scientists working in the field of cell biology, tissue engineering, and drug development. The SMCC-7721 ensures the integrity and traceability of cell cultures, enabling reliable and reproducible research results.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by National Collection of Authenticated Cell Cultures (9 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Hep3b, by National Collection of Authenticated Cell Cultures (5 mentions)
Mhcc 97h, by National Collection of Authenticated Cell Cultures (5 mentions)
Mhcc 97l, by National Collection of Authenticated Cell Cultures (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions)
High glucose dmem, by Thermo Fisher Scientific (3 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (2 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Bel 7404, by National Collection of Authenticated Cell Cultures (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Lonsera (1 mentions)
Bel 7402, by National Collection of Authenticated Cell Cultures (1 mentions)
Sk hep1, by National Collection of Authenticated Cell Cultures (1 mentions)
Mhcc97h, by Thermo Fisher Scientific (1 mentions)
13 protocols using smcc 7721
1
Characterization of Human Hepatocellular Carcinoma
2
Culturing Human Liver Cell Lines
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All HCC cells including Huh7, Hep3B, HepG2, MHCC-97L, SMCC-7721, and immortalized human hepatic cell LO2 were obtained from the Type Culture Collection of the Chinese Academy of Sciences. All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/high glucose(Gibco, USA), supplement 10% FBS (Gibco, USA) and penicillin–streptomycin (100 U/mL and 100 ug/mL, respectively) and maintained at 37˚C in 5% CO2 humidified incubator.
3
Constructing Stable Knockdown Cell Lines
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The human hepatocellular carcinoma cell lines HepG2, L-O2, SMCC-7721, HEP3B and HUH7 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). MHCC97-L (97 L), MHCC 97H (97H) and HCC-LM3 (LM3) cells were kindly provided by Prof. Zhaoyou Tang (Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China). The cells were grown in high-glucose Dulbecco's Modified Eagle Medium (DMEM; Invitrogen, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 1% penicillin/streptomycin. All the cultures were incubated at 37 °C with 5% CO2.
To construct both the circPTGR1 and the corresponding parent linear PTGR1 stable knockdown cell lines, short hairpin RNAs (shRNAs) containing the si-circPTGR1 (GAAGAAAGCGTCTCCTGAT), si-PTGR1(GGACCCTGAAGAAGCACTT) sequence were cloned into lentiviral vectors (Forevergen, Guangzhou, China). A vector containing the si-NC (CTTTCTCCGAACGTG TCAC) sequence was used as a negative control. All constructed lentivirus vectors were transfected with the packaging plasmids pGag/Pol, pRev, and pVSV-G into 293 T cells with Lipofectamine 2000. Viruses in cell supernatant were collected at 48 h and 72 h post transfection and transduced to LM3 cells, in order to generate the shcircPTGR1, shPTGR1, and control NC cell lines.
To construct both the circPTGR1 and the corresponding parent linear PTGR1 stable knockdown cell lines, short hairpin RNAs (shRNAs) containing the si-circPTGR1 (GAAGAAAGCGTCTCCTGAT), si-PTGR1(GGACCCTGAAGAAGCACTT) sequence were cloned into lentiviral vectors (Forevergen, Guangzhou, China). A vector containing the si-NC (CTTTCTCCGAACGTG TCAC) sequence was used as a negative control. All constructed lentivirus vectors were transfected with the packaging plasmids pGag/Pol, pRev, and pVSV-G into 293 T cells with Lipofectamine 2000. Viruses in cell supernatant were collected at 48 h and 72 h post transfection and transduced to LM3 cells, in order to generate the shcircPTGR1, shPTGR1, and control NC cell lines.
4
Culturing Human Liver Cancer Cell Lines
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The human HCC cell lines SMCC-7721 (7721), MHCC 97H (97H), MHCC 97L (97L), Huh7, HepG2 (G2), HCC-LM3 (LM3), and BEL7404 (7404) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco Company, USA) supplemented with 10% fetal bovine serum (FBS; Gibco Company, USA) and 1% penicillin/streptomycin. All the cultures were incubated at 37 °C with 5% CO2.
5
Surgical Resection of Hepatocellular Carcinoma
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We followed up 43 patients who were diagnosed as hepatocellular carcinoma through liver puncture and histopathology in the First A liated Hospital of Xi'an Jiaotong University. Subsequently, these patients received the oxaliplatin-based systemic chemotherapy. When the tumor was suppressed enough to correspond to surgical indications, hepatectomy was performed. Carcinoma tissues and para-carcinoma tissues (more than 3cm distal to the edge of tumor) were collected from surgical specimens. The survival time of patients was followed up and recorded. All samples were collected from consenting individuals and on the basis of the protocol approved by the Ethics Committee of the First A liated Hospital of Xi'an Jiaotong University.
The human cell lines (MHCC-97L, MHCC-97H, HepG2, Hep3B, Huh7, SMCC-7721 and LO2) were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All these cells were maintained in DMEM (HyClone, USA) with 10% FBS (HyClone, USA) supplemented with 1% streptomycinpenicillin at 37°C and 5% CO2.
The human cell lines (MHCC-97L, MHCC-97H, HepG2, Hep3B, Huh7, SMCC-7721 and LO2) were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All these cells were maintained in DMEM (HyClone, USA) with 10% FBS (HyClone, USA) supplemented with 1% streptomycinpenicillin at 37°C and 5% CO2.
6
Culturing Human Hepatocellular Carcinoma
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Human HCC cell lines (HepG2 and SMCC-7721) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured according to the instructions from American Type Culture Collection (ATCC). The cells were maintained in high-glucose DMEM (Gibco) supplemented with 10% fetal calf serum (Gibco). The cells were incubated at 37°C in a humidified chamber containing 5% CO2.
7
Culturing Human Liver Cancer Cell Lines
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Human HCC cell lines (SMCC-7721 and HepG2) were bought from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% FBS and 100 units/mL of penicillin and 100 mg/mL of streptomycin (Invitrogen, Carlsbad, CA). The cells were maintained in high-glucose DMEM (Gibco) supplemented with 10% fetal calf serum (Gibco). The cells were then incubated at 37°C in a humidified chamber containing 5% CO2.
8
Culturing Liver Cancer Cell Lines
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The liver cancer cell lines SK-Hep-1, SMCC-7721, HCCLM3, MHCC-97L, BEL-7402, and Huh-7 and the normal liver cell line L02 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, #L110KJ, Shanghai BasalMedia Technologies Co., Ltd., Shanghai, China) containing 10% fetal bovine serum (FBS, Lonsera, #S711-001S, Uruguay).
9
Culturing Human Liver Cell Lines
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The human HCC cell lines (HepG2, SMCC-7721, MHCC-97H) and normal liver cell line (LO2) were purchased from China Center for Type Culture Collection (Wuhan, China). These cell lines were cultured using Dulbecco's Modi ed Eagle's Medium (DMEM; Thermo Fisher Scienti c, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scienti c, Waltham, MA, USA), 100 U/ml penicillin and 100 ng/ml streptomycin in a humidi ed atmosphere of 5% (v/v) CO 2 at 37°C.
10
GNL2 Knockdown in LIHC Cell Lines
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The human LIHC cell lines MHCC97-H and SMCC-7721 were obtained from the China Center for Type Culture Collection in 2020 and authenticated by short tandem repeat analysis. All cells were routinely cultured in DMEM medium (Thermo Fisher Scientific, Inc., USA) supplemented with 10% FBS (Thermo Fisher Scientific, Inc., USA) in an incubator with 5% CO2 at 37°C. SiRNA targeting GNL2 (si-GNL2) and a negative control siRNA (si-NC) were acquired from GeneCopoeia, Inc. (USA). When MHCC97-H and SMCC-7721 cells confluence reached up to 80% on the following day, they were transfected with indicated siRNAs utilizing Lipofectamine 2000 (Invitrogen, USA) as the manual described. Reduced efficiency of GNL2 was determined by qRT-PCR at 48 h posttransfection. The siRNAs used were as follows: si-GNL2-1, 5′-CACGTGTGATTAAGCAGTCATCATT-3′; si-GNL2-2, 5′-CCATACAAAGTTGTCATGAAGCAAA-3′; si-NC, 5′-UUCUCCGAACGUGUCACGUTT-3′.
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