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4 hydroxybenzoic acid

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4-hydroxybenzoic acid is a chemical compound with the formula C₆H₄(OH)COOH. It is a white crystalline solid that is used as a preservative and in the production of various chemicals and pharmaceuticals.

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Lab products found in correlation

Caffeic acid, by Merck Group (2 mentions) Syringic acid, by Merck Group (2 mentions) Xanthine oxidase, by Merck Group (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Juglone, by Merck Group (1 mentions) Dihydrocaffeic acid, by Merck Group (1 mentions) 2 4 dihydroxybenzoic acid, by Merck Group (1 mentions) Gallic acid, by Thermo Fisher Scientific (1 mentions) Ferulic acid, by Merck Group (1 mentions) Vanillic acid, by Merck Group (1 mentions) 3 4 dihydroxybenzoic acid, by Merck Group (1 mentions) Lawsone, by Merck Group (1 mentions) Acetone, by Thermo Fisher Scientific (1 mentions) Protocatechuic acid, by Merck Group (1 mentions) Sea salt, by Merck Group (1 mentions) Kanamycin, by Carl Roth (1 mentions) Diaion hp 20, by Merck Group (1 mentions) Naringenin, by Merck Group (1 mentions) Restriction enzyme, by New England Biolabs (1 mentions) Acetone d6, by Deutero (1 mentions)

5 protocols using 4 hydroxybenzoic acid

1

Enzymatic Oxidation of Halogenated Phenols

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Reagents of the highest grade available were used without purification. 3-Iodo-L-tyrosine (I-Tyr), 2-iodophenol (2IP), m-cresol and formic acid (88%) were obtained from Acros Organics. 3-Fluoro-L-tyrosine (F-Tyr) was purchased from Astatech, Inc. 4-Hydroxybenzoic acid was obtained from Alfa Aesar. 4-Cyano-2-iodophenol (2IPCN) was purchased from Pharmabridge, Inc. 4-Amino-2-iodophenol (4A2IP), xanthine and xanthine oxidase were obtained from Sigma-Aldrich.
Ingavat N., Kavran J.M., Sun Z, & Rokita S.E. (2017). Active Site Binding is not Sufficient for Reductive Deiodination by Iodotyrosine Deiodinase. Biochemistry, 56(8), 1130-1139.
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2

Quantification of Natural Phenols by HPLC

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Acetonitrile, water and methanol (LC-MS Chromasolv grade, >99.9% purity) used for sample pre-treatment and as HPLC-ESI-MS eluents were from Fluka (Milan, Italy). Eluents for HPLC-DAD analysis were acetonitrile (Chromasolv for HPLC, >99.8%) from Sigma Aldrich (Milan, Italy) and water (RPE) from Carlo Erba. Formic acid 98% purity was from J.T. Baker. Gallic acid (more than 99% purity) and ellagic acid dehydrate (97% purity) were from Alpha Aesar (Lancaster, England), 4-hydroxybenzoic acid (99% purity), 3,4-dihydroxybenzoic acid (>97% purity), 2,4-dihydroxybenzoic acid (97% purity), dihydrocaffeic acid (98% purity), ferulic acid (99% purity), syringic acid (98% purity), vanillic acid (97% purity), caffeic acid (97% purity), juglone (97% purity) and lawsone (97% purity) were from Sigma Aldrich (Milan, Italy).
The stock solution contained 13 natural phenols in methanol (100 µg/g). Aliquots of the stock solution were diluted with water to obtain working standards for method development. The HPLC-DAD requires a standard concentration in the range of 10 µg/g, while HPLC-ESI-Q-ToF of 3 µg/g. Solutions were stored at −18°C.
Restivo A., Degano I., Ribechini E, & Colombini M.P. (2014). Development and Optimisation of an HPLC-DAD-ESI-Q-ToF Method for the Determination of Phenolic Acids and Derivatives. PLoS ONE, 9(2), e88762.
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3

Elderberry Phytochemical Characterization

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Commercial elderberry ACN powder (Sambucus nigra) was provided by D. D. Williamson (Louisville, KY, USA). Acetone was obtained from Fisher Scientific (Waltham, MA, USA). Caffeic acid and syringic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). Protocatechuic acid and phloroglucinaldehyde were obtained from Aldrich Chemistry (Milwaukee, WI, USA). 4-Hydroxybenzoic acid was obtained from Acros Organics (Fair Lawn, NJ, USA). All reagents used were of analytical grade or higher unless otherwise noted.
Voss D.M., Miyagusuku-Cruzado G, & Giusti M.M. (2022). Comparing the thermal stability of 10-carboxy-, 10-methyl-, and 10-catechyl-pyranocyanidin-3-glucosides and their precursor, cyanidin-3-glucoside. NPJ Science of Food, 6, 16.
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4

Isolation and Synthesis of Natural Compounds

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Sea salts and (±)-naringenin were purchased from Sigma-Aldrich (Steinheim, Germany). 4-hydroxybenzoic acid and 1,6-dihydroxynaphthalene were obtained from Acros Organics (New Jersey, USA). 1-hydroxyphenazine was from TCI (Zwijndrecht, Belgium), kanamycin was from Roth (Karlsruhe, Germany), and chloramphenicol was from Genaxxon BioScience (Biberach, Germany). Diaion HP-20 was received from Supelco (Bellefonte, USA) and PCA from InFarmatik (Hungary). Acetone-d6 was purchased from Deutero (Kastellaun, Germany). Reference samples of JBIR-46 and -47 were isolated as described previously [14] (link). Flaviolin was isolated as described by Gross et al. [46] (link) and DMAPP, geranyl diphosphate, and farnesyl diphosphate were synthesized as described previously [47] . Restriction enzymes were bought from New England Biolabs (Frankfurt am Main, Germany).
Zeyhle P., Bauer J.S., Kalinowski J., Shin-ya K., Gross H, & Heide L. (2014). Genome-Based Discovery of a Novel Membrane-Bound 1,6-Dihydroxyphenazine Prenyltransferase from a Marine Actinomycete. PLoS ONE, 9(6), e99122.
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5

Comprehensive Reagents Inventory for Experimental Protocols

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Dimethylsulfoxide (Me2SO), reduced L-glutathione (98%+), uric acid (sodium salt, analytical grade), sodium bisulfite (A.C.S), chelex 100 (sodium form) and hydrogen peroxide were obtained from Sigma-Aldrich (St. Louis, MO). [y-32P]ATP (6000 Ci/mmol) was purchased from ICN Biochemicals, Inc. (Costa Mesa, CA). Other molecular biological reagents were purchased from QIAGEN (Valencia, CA) and Roche Clinical Laboratories (Indianapolis, IN). Sodium chloride (A.C.S.), sodium benzoate (A.C.S.), citric acid (A.C.S.), sodium phosphate (A.C.S.), potassium phosphate (A.C.S.), acetonitrile (HPLC grade) and perchloric acid (Optima) were from Fisher Scientific. L-ascorbic acid (sodium salt, 99%) and 4-hydroxybenzoic acid (99%) were from Acros Organics. Iron (II) sulfate (FeSO4•7H2O, A.C.S.) was from EMD. Printex-90, a nano-sized carbon black (CB), was obtained from Degussa (Frankfurt, Germany). The manufacturer presented the primary particle diameter of Printex 90 as 14 nm.
Vogel C.F., Charrier J.G., Wu D., McFall A.S., Li W., Abid A., Kennedy I.M, & Anastasio C. (2016). Physicochemical properties of iron oxide nanoparticles that contribute to cellular ROS-dependent signaling and acellular production of hydroxyl radical. Free radical research, 50(11), 1153-1164.
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