Dulbecco modified eagle medium (dmem)

A 40 mg/ml stock solution of hydrophilic Pt-NP_PVP powder (particle size: 3 nm, PlasmaChem, Berlin, Germany) was dispersed in sterile Aqua bidest. by water bath sonication for 15 min. The Pt-NP_PVP stock solution was diluted to the desired concentrations in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Bio&Sell, Germany) completed with 10% fetal calf serum (FCS, Bio&Sell, Germany). According to Kalinec et al. the cell culture medium was not supplemented with any antibiotics [51 (link)].

Embryonic rat-heart-derived H9c2-myoblasts obtained from America Tissue Type Collection (Manassas, VA, USA, cat. CRL-1446) were cultured using Dulbecco’s Modified Eagle’s Medium (DMEM; Bio&SELL, Feucht/Nürnberg, Germany, cat. BS.11971083) supplemented with 10% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin (Bio&SELL, Feucht/Nürnberg, Germany, cat. BS.4681080) and grown at 37 °C in a 5% CO₂ humidified atmosphere. The medium was changed every 3–4 days, and cells were subcultured when at 70–80% confluence. Before treatment, H9c2 cells were seeded in a density of 2.1 × 10⁴ cells/cm² in 2 mL complete DMEM medium and grown for 24 h.