Discovery workbench data analysis software

The analysis of inflammatory markers has been described previously.^{25 (link)} In brief, a U-PLEX assay based on an electrochemiluminescent detection method (Meso Scale Diagnostics, Rockville, MD) was used to analyze the concentrations of 71 cytokines in plasma samples from patients (n = 13) with peripheral NeuP and healthy controls (n = 13). A MESO QUICKPLEX SQ 120 instrument equipped with DISCOVERY WORKBENCH data analysis software (Meso Scale Diagnostics, Rockville, MD) was used to collect and analyze data. Electrochemiluminescent signals from calibrators were fitted to a weighted 4-parametric logistic model to form standard curves.

The concentrations of 71 cytokines, chemokines, and growth factors were analyzed using a U-PLEX assay based on an electrochemiluminescent detection method (Meso Scale Diagnostics, Rockville, MD) according to the manufacturer's recommendations. Data were collected and analyzed using MESO QUICKPLEX SQ 120 instrument equipped with DISCOVERY WORKBENCH data analysis software (Meso Scale Diagnostics). Samples were thawed on the day of analysis, blinded, and were randomly mixed.

The concentrations of inflammatory proteins in saliva samples were analyzed from the last six healthy control subjects from the original group of 16 controls with the purpose to investigate the possibility to detect these proteins in the samples. The samples were analyzed using a commercially available V-PLEX human biomarkers (Meso Scale Diagnostics, Rockville, MD, USA) multiplex assay. The assay contained 39 biomarkers (see Table 4) and was based on an electrochemiluminescent detection method and performed exactly according to the manufacturer’s recommendations. Data were collected and analyzed using MESO QUICKPLEX SQ 120 instrument equipped with DISCOVERY WORKBENCH^® data analysis software (Meso Scale Diagnostics, Rockville, MD, USA).
The precisions based on both intra- and interassay coefficient of variations were <10% within the detection limits. The detection limit for each investigated biomarker is shown in Table 4 [24 (link),25 (link)].
Total protein concentration was measured using 2D Quant-kit (GE Healthcare Biosciences AB, Uppsala, Sweden), according to the manufacturer’s protocol.