Human rat beta amyloid 40 elisa kit

Human umbilical vain endothelial cells (HUVECs) from Cell Applications, Inc. were cultured in endothelial cell basal medium (ECBM) with endothelial cell growth supplement containing fetal bovine serum in 35-mm dishes (3 x 10⁵ cells/dish). To measure Aβ40 in the medium after seeding for 24 h, the culture medium was changed to ECBM containing 3% (v/v) heat-inactivated pooled human serum purchased from Innovative Research, Inc., 5 ng/ml of rhFGF-basic, 5 ng/ml of rhEGF, 0.75 U/ml of heparin and 0.1 μg/ml of hydrocortisone. Cells were incubated at 37°C in 5% CO2. Culture supernatant from HUVECs was harvested at 24, 48 and 72 h after changing the medium containing pooled normal human serum. The production of Aβ40 from HUVECs was measured using the Human/Rat beta-Amyloid (40) ELISA kit (Wako pure chemicals, co, ltd.) according to the manufacturer’s protocol.