RPMI-1640 medium was purchased from Thermo Fisher (Shanghai, China). CCK-8 and Hoechst 33342 solution were obtained from Dojindo Laboratories (Tokyo, Japan). LPS, LY294002, and LiCl were obtained from Sigma-Aldrich (St. Louis, MO, USA). Annexin V-FITC/Propidium Iodide were obtained from Biouniquer Technology Co., Ltd. Antibodies of AKT, phospho-AKT, GSK-3β, P-GSK-3β, Bax, Bcl-2, and active caspase-3 were obtained from Cell Signaling Technology (Beverly, MA, USA). CLI-095 was obtained from Invivogen Biotechnology (San Diego, CA, USA). RNAiso Plus, SYBR Green Premix Ex Taq II, and PrimeScript RT Reagent Kit (Perfect Real Time) were purchased from TaKaRa (Takara Bio Inc., Shiga, Japan). PCR primers were synthesized at RQ Biotech (Shanghai, China).
Antibodies of akt
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies of AKT are a type of laboratory equipment used in various research and diagnostic applications. AKT, also known as Protein Kinase B, is a key signaling molecule involved in cellular processes such as cell growth, proliferation, and metabolism. These antibodies are designed to specifically detect and bind to AKT, allowing researchers to study its expression, activation, and role in different biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Sybr green premix ex taq 2, by Takara Bio (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Lithium chloride (licl), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Ly294002, by Merck Group (1 mentions)
Rnaiso plus, by Takara Bio (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Active caspase 3, by Cell Signaling Technology (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
Hoechst 33342 solution, by Dojindo Laboratories (1 mentions)
Cck 8, by Dojindo Laboratories (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Caveolin 1, by Cell Signaling Technology (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
2 protocols using antibodies of akt
1
Molecular Mechanisms of Immune Cell Apoptosis
2
Endothelial Cell Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Passage-4 human umbilical vein endothelial cells (HUVECs) were cultured in an endothelial cell medium (both from ScienCell Research Laboratories, Carlsbad, Calif) supplemented with 5% fetal bovine serum, 1% growth factors, and 1% penicillin/streptomycin. After serum starvation with 0.5% fetal bovine serum for 24 hours, HUVECs were treated with MPs (4.6 3 10 6 per mL) for 1 hour. Cellular proteins were obtained, and western blotting was performed as described elsewhere. 10 Passage-4 HUVECs treated with phosphate-buffered saline were used as controls.
Antibodies of Akt, phosphorylation of Akt, phosphorylation of eNOS at Ser1177 (serine 1177 site), and caveolin-1 were from Cell Signaling Technology Inc (Danvers, Mass). Antibody of eNOS (sc-654),
Antibodies of Akt, phosphorylation of Akt, phosphorylation of eNOS at Ser1177 (serine 1177 site), and caveolin-1 were from Cell Signaling Technology Inc (Danvers, Mass). Antibody of eNOS (sc-654),
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!