Silicone oil (SiO) with a viscosity of 10 mPa s, a density of 0.93356 kg/m3 and a refractive index (RI) of 1.39922, and olive oil (OO) with a density of 0.90865 kg/m3 and RI = 1.46689 were obtained from Sigma-Aldrich. Tetrachloroauric acid trihydrate (HAuCl4 3H2O) was purchased from VWR. A low-molecular-weight chitosan with a degree of deacetylation of 81.2% was acquired from Sigma-Aldrich. Iron(III)chloride hexahydrate (FeCl3 6H2O) was obtained from Fluka and iron sulfate heptahydrate (FeSO4 7H2O) from Roth. For all experiments, purified water from a Milli-Q Reference A+ System (Millipore) was used.
Milli q reference a system
Manufactured by Merck Group
Sourced in Germany, United States
The Milli-Q Reference A+ system is a water purification device designed to produce high-quality ultrapure water. It utilizes a combination of filtration and deionization technologies to remove impurities from the input water, resulting in water that meets the stringent purity requirements for various applications, such as laboratory experiments, analytical testing, and other scientific procedures.
Automatically generated - may contain errors
Lab products found in correlation
Sodium carbonate, by Merck Group (3 mentions)
Acetonitrile chromasolv, by Merck Group (2 mentions)
Epicatechin, by Merck Group (2 mentions)
Aliquat 336, by Thermo Fisher Scientific (2 mentions)
Di isobutylketone dibk, by Thermo Fisher Scientific (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Avantor (2 mentions)
Olive oil, by Merck Group (1 mentions)
Low molecular weight chitosan, by Merck Group (1 mentions)
Silicone oil, by Merck Group (1 mentions)
Galβ1 4glcnac 6s, by Seikagaku (1 mentions)
Palmatine, by Merck Group (1 mentions)
Tetrahydropalmatine, by Merck Group (1 mentions)
Epigallocatechin, by Merck Group (1 mentions)
Copper sulfate, by Merck Group (1 mentions)
Folin ciocalteu, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
15 protocols using milli q reference a system
1
Synthesis of Metallic Nanoparticles
2
Analytical Procedures for Compound Identification
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The compounds used for the present study are presented in the Supporting Information. Water for ultrafiltration, UHPLC-MS/MS and HPIC measurements was purified via a Milli-Q Reference A+ system (Millipore, Schwalbach, Germany). Solvents (acetonitrile, methanol) in LC-MS grade were purchased from Merck (Darmstadt, Germany). Stable isotope-labeled amino acids were obtained from Eurisotop (Saarbrücken, Germany).
3
Glycosaminoglycan Quantification Protocol
4
Extraction and Quantification of Alkaloids
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Ethanol, methanol and formic acid of HPLC Ultra-Gradient grade were purchased from Carlo Erba (Val de Reuil, France). Ultrapure water (18.2 MΩ) for HPLC analysis was obtained from a Milli-Q Reference A+ system (Millipore, CO., Bedford, MA, USA). Palmatine, roemerine and tetrahydroPalmatine were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France), Ambinter (Orléans, France) and Phytolab (Vestenbergsgreuth, Germany), respectively.
5
Colorimetric Assay Reagents for Analysis
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Reagents for colorimetric assays (Folin–Ciocalteu, DPPH, pyrocatechol violet and cinchonine hemisulfate, Trolox®, EDTA, sodium carbonate, sodium acetate, and copper sulfate) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile CHROMASOLV® (gradient grade, for HPLC, ≥99.9%) for LC-MS analysis was purchased from Sigma-Aldrich, and Milli-Q H2O was obtained in the laboratory using a Milli-Q Reference A+System (Merck Millipore, Burlington, MA, USA). Standards for LC-MS (gallic acid, catechin, epicatechin, ellagic acid, and (epi) gallocatechin) were purchased from Sigma-Aldrich.
6
Quantitative Peptide Analysis by LC-MS
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Formic acid and acetonitrile
in LC-MS grade were obtained from Merck (Darmstadt, Germany). The
synthetic reference peptides (purity ≥80%) were purchased from
Peptides and Elephants (Hennigsdorf, Germany). Deuterium oxide (D2O) was supplied from Sigma-Aldrich (Steinheim, Germany), and
deionized water for chromatography was purified using a Milli-Q Reference
A+ system from Merck Millipore (Schwalbach, Germany). RotiQuant assay
solution was purchased from Carl Roth (Karlsruhe, Germany). The quinoa
grains were obtained from Münchner Bauern Genossenschaft (Munich,
Germany). Buffer constituents were purchased from Carl Roth (Karlsruhe,
Germany) or Merck.
in LC-MS grade were obtained from Merck (Darmstadt, Germany). The
synthetic reference peptides (purity ≥80%) were purchased from
Peptides and Elephants (Hennigsdorf, Germany). Deuterium oxide (D2O) was supplied from Sigma-Aldrich (Steinheim, Germany), and
deionized water for chromatography was purified using a Milli-Q Reference
A+ system from Merck Millipore (Schwalbach, Germany). RotiQuant assay
solution was purchased from Carl Roth (Karlsruhe, Germany). The quinoa
grains were obtained from Münchner Bauern Genossenschaft (Munich,
Germany). Buffer constituents were purchased from Carl Roth (Karlsruhe,
Germany) or Merck.
7
Solvent Extraction Reagent Acquisition
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Aliquat
336 was purchased from Thermo Fisher
Scientific (Merelbeke, Belgium). KBr (≥99%) and di-isobutyl
ketone (DIBK, >96%) were acquired from Acros Organics (Geel, Belgium).
Potassium iodide (KI) (≥99.5%) was bought from Honeywell Riedel
de Haën (Seelze, Germany). KSCN (>99%) and KNO3 (>99%)
were purchased from Chem-Lab (Zedelgem, Belgium). NaHSO4 (>93%) was acquired from Carl Roth (Karlsruhe, Germany). Water
was
always of ultrapure quality (Milli-Q water), deionized with a Merck
Millipore Milli-Q Reference A+ system. All chemicals were used as
received, without any further purification.
336 was purchased from Thermo Fisher
Scientific (Merelbeke, Belgium). KBr (≥99%) and di-isobutyl
ketone (DIBK, >96%) were acquired from Acros Organics (Geel, Belgium).
Potassium iodide (KI) (≥99.5%) was bought from Honeywell Riedel
de Haën (Seelze, Germany). KSCN (>99%) and KNO3 (>99%)
were purchased from Chem-Lab (Zedelgem, Belgium). NaHSO4 (>93%) was acquired from Carl Roth (Karlsruhe, Germany). Water
was
always of ultrapure quality (Milli-Q water), deionized with a Merck
Millipore Milli-Q Reference A+ system. All chemicals were used as
received, without any further purification.
8
Cultivation of Marine Bacterium Pseudoalteromonas pacifica
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P. pacifica DSM 14875T was maintained in VY2-SWS agar (fresh Baker’s yeast 5 g/L, NaCl 20 g/L, Bacto Agar (Difco) 15 g/L [8 (link)], HEPES 2.38 g/L, pH adjusted 7.2 with NaOH). The commercial yeast was prepared by washing five times with 50 mL Milli-Q deionized water (MQ-H2O, obtained with Milli-Q Reference A + System®, Merck Millipore, Darmstadt, Germany). The sea water salt (SWS) solution was prepared according a previous study with a reduced iron(III) citrate concentration [8 (link)]. Liquid cultivations were prepared in RG244 medium (NaCl 20 g/L, ferric citrate 0.01 g/L, MgSO4·7H2O 8 g/L, CaCl2·2H2O 1 g/L, KCl 0.5 g/L, NaHCO3 0.16 g/L, H3BO3 0.02 g/L, KBr 0.08 g/L, SrCl2·6H2O 0.03 g/L, di-Na-β-glycerophosphate 0.01 g/L, 1 mL trace element solution (ZnSO4·7H2O 0.10 g/L, MnCl2·4H2O 0.03 g/L, H3BO3 0.30 g/L, CoCl2·6H2O 0.20 g/L, CuCl2·2H2O 0.01 g/L, NiCl2·6H2O 0.02 g/L, NaMoO4·2H2O 0.03 g/L, EDTA 0.5 g/L, FeSO4·7H2O 0.2 g/L) skim milk 2 g/L, HEPES 2.38 g/L, pH adjusted to 7.2 with NaOH before autoclaving).
9
Chicory Leaf Phenolic Compound Extraction
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Frozen C. intybus leftovers were kindly provided by BioSphere (Bertinoro, Italy).
EtOH (ACS grade, ≥99%) (Sigma–Aldrich, Milan, Italy) and methanol (MeOH, HPLC, ≥99.9%) were used for extractions, total phenolic micro-assays and SPE purifications. Acetonitrile for HPLC analyses (MeCN, HPLC Plus, ≥99.9%) was purchased from Sigma–Aldrich, while Milli-Q H2O was obtained in the laboratory from a Milli-Q Reference A + System (Merck Millipore, Darmstadt, DE, USA). Glacial acetic acid (AcOH, ≥96%) was purchased from Merck (Darmstadt, DE, USA). Standards of gallic, p-hydroxybenzoic, caffeic, ellagic, p-coumaric, ferulic, chlorogenic, vanillic, syringic, protocatechuic and 3,4-dimethoxybenzoic (veratric) acids, (−)-epicatechin, apigenin-3-glucoside, luteolin-3-glucoside as well as the Folin–Ciocalteau reagent and sodium carbonate for total phenolic assays were purchased from Sigma-Aldrich (Milan, Italy).
EtOH (ACS grade, ≥99%) (Sigma–Aldrich, Milan, Italy) and methanol (MeOH, HPLC, ≥99.9%) were used for extractions, total phenolic micro-assays and SPE purifications. Acetonitrile for HPLC analyses (MeCN, HPLC Plus, ≥99.9%) was purchased from Sigma–Aldrich, while Milli-Q H2O was obtained in the laboratory from a Milli-Q Reference A + System (Merck Millipore, Darmstadt, DE, USA). Glacial acetic acid (AcOH, ≥96%) was purchased from Merck (Darmstadt, DE, USA). Standards of gallic, p-hydroxybenzoic, caffeic, ellagic, p-coumaric, ferulic, chlorogenic, vanillic, syringic, protocatechuic and 3,4-dimethoxybenzoic (veratric) acids, (−)-epicatechin, apigenin-3-glucoside, luteolin-3-glucoside as well as the Folin–Ciocalteau reagent and sodium carbonate for total phenolic assays were purchased from Sigma-Aldrich (Milan, Italy).
10
Solvent Extraction of Rare-Earth Elements
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Polyethylene glycol with a number-average molecular mass of 200
g mol–1 (PEG 200) was obtained from J&K Scientific
(Zedelgem, Belgium). NdCl3·6H2O (99.9%)
was purchased from Strem Chemicals (Newburyport, USA), Nd2O3 (99.9%) from Alfa-Aesar (Geel, Belgium), DyCl3·6H2O (99.9%),) from abcr GmbH (Karlsruhe, Germany),
and Dy2O3 from Strem Chemicals (Newburyport,
USA). Cyanex 923 was supplied by Solvay (Toulouse, France). Oxalic
acid (>99%) and LiCl (99.9%) were ordered from Sigma-Aldrich (Diegem,
Belgium). Hydrochloric acid (37%) was purchased from VWR Chemicals
(Haasrode, Belgium). The aliphatic hydrocarbon diluent Shell GTL Solvent
GS190 (GS190), composed of C10–C13n- and iso-alkanes with a boiling range of 187–218
°C, was supplied by Shell (Rotterdam, Netherlands). 1-Decanol
(99%) was ordered from Acros Organics (Geel, Belgium). 1-Butanol (99%)
was purchased from Thermo Fisher Scientific (Geel, Belgium). The silicone
solution in isopropanol was supplied by SERVA Electrophoresis GmbH
(Heidelberg, Germany). The gallium and scandium standard (1000 mg
L–1 in 2–5% HNO3) were obtained
from Chem-Lab NV (Zedelgem, Belgium). Triton X-100 was obtained from
Merck KGaA (Darmstadt, Germany). Water was always of ultrapure quality,
deionized to a conductivity of <0.055 μS cm–1 (298.15 K) with a Merck Millipore Milli-Q Reference A+ system. All
chemicals were used as received without any further purification.
g mol–1 (PEG 200) was obtained from J&K Scientific
(Zedelgem, Belgium). NdCl3·6H2O (99.9%)
was purchased from Strem Chemicals (Newburyport, USA), Nd2O3 (99.9%) from Alfa-Aesar (Geel, Belgium), DyCl3·6H2O (99.9%),) from abcr GmbH (Karlsruhe, Germany),
and Dy2O3 from Strem Chemicals (Newburyport,
USA). Cyanex 923 was supplied by Solvay (Toulouse, France). Oxalic
acid (>99%) and LiCl (99.9%) were ordered from Sigma-Aldrich (Diegem,
Belgium). Hydrochloric acid (37%) was purchased from VWR Chemicals
(Haasrode, Belgium). The aliphatic hydrocarbon diluent Shell GTL Solvent
GS190 (GS190), composed of C10–C13n- and iso-alkanes with a boiling range of 187–218
°C, was supplied by Shell (Rotterdam, Netherlands). 1-Decanol
(99%) was ordered from Acros Organics (Geel, Belgium). 1-Butanol (99%)
was purchased from Thermo Fisher Scientific (Geel, Belgium). The silicone
solution in isopropanol was supplied by SERVA Electrophoresis GmbH
(Heidelberg, Germany). The gallium and scandium standard (1000 mg
L–1 in 2–5% HNO3) were obtained
from Chem-Lab NV (Zedelgem, Belgium). Triton X-100 was obtained from
Merck KGaA (Darmstadt, Germany). Water was always of ultrapure quality,
deionized to a conductivity of <0.055 μS cm–1 (298.15 K) with a Merck Millipore Milli-Q Reference A+ system. All
chemicals were used as received without any further purification.
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