Graphene oxide powder was purchased from Nanjing XF NANO Materials Tech Co., Ltd (China). Mitotracker red, Lysotracker red, Calcein AM/PI kit, Annexin V-FITC/PI apoptosis staining kit were purchased from Solarbio (Beijing, China). IR-780 iodide (IR780), ferritin (FRT) from equine spleen, resveratrol (RSV, ≥99%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimidehydrochloride (EDC), N-hydroxysuccinimide (NHS) and 6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (USA). All other chemicals used in the study were from Aladdin (Shanghai, China).
Calcein am pi kit
Manufactured by Solarbio
Sourced in China
The Calcein-AM/PI kit is a fluorescence-based assay designed to detect and distinguish between live and dead cells. Calcein-AM is a cell-permeant dye that is converted to a green fluorescent compound by esterases in live cells, while propidium iodide (PI) is a red fluorescent dye that can only enter cells with compromised membranes, staining the nuclei of dead or dying cells. This kit allows for the simultaneous visualization and quantification of live and dead cells.
Automatically generated - may contain errors
Lab products found in correlation
Lyso tracker red, by Solarbio (1 mentions)
Mitotracker red, by Solarbio (1 mentions)
6 diamidino 2 phenylindole dapi, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Resveratrol, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions)
Collagenase type 1, by Merck Group (1 mentions)
Membrane and cytosol protein extraction kit, by Beyotime (1 mentions)
Anti cd31 antibody, by Proteintech (1 mentions)
N cadherin antibody, by Proteintech (1 mentions)
Epcam antibody, by Proteintech (1 mentions)
Rat tail tendon collagen type 1, by Solarbio (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Interleukin 6 (il 6), by Jiangsu Meimian (1 mentions)
Il 1β, by Jiangsu Meimian (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Beyotime (1 mentions)
Tnf α elisa kit, by Jiangsu Meimian (1 mentions)
Leica tcs spe, by Leica camera (1 mentions)
Oxaliplatin, by MedChemExpress (1 mentions)
4 protocols using calcein am pi kit
1
Graphene Oxide-Based Theranostic Nanoplatform
2
Multifunctional Nanoparticles for Cancer Therapy
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Ethylene glycol (EG), poly(vinyl pyrrolidone) (PVP), silver trifluoroacetate (CF3COOAg), doxorubicin hydrochloride (Dox), 2-iminothiolane (Traut’s reagent), MTT and iohexol were obtained from Aladdin Co., Ltd. (Shanghai, China). Sodium hydrosulfide hydrate (NaHS) was purchased from Saen Chemical Technology Co., Ltd (Shanghai, China). Chloroauric acid (HAuCl4) was obtained from Shanghai Titan Scientific Co., Ltd. (Shanghai, China). Membrane and Cytosol Protein Extraction Kit, DCFH-DA, 4′6-diamidino-2-phenylindole (DAPI) and Annexin V-FITC/PI Apoptosis Detection Kit were purchased from Beyotime (China). Collagenase (Type I) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rattailtendon collagen type I and Calcein-AM/PI kit were purchased from Solarbio (Beijing, China). DSPE-PEG2k-MAL was purchased from ShangHai PonsureBio Tech. Inc. (Shanghai, China). SH-PEG-Cy5.5 was purchased from ShangHai ToYongBio Tech.Inc. (Shanghai, China). N-cadherin antibody, anti-CD47 antibody, EpCAM antibody, ATP1A1 antibody, collagen type I antibody and anti-CD31 antibody were purchased from Proteintech (Wuhan, China). Nab-paclitaxel was purchased from Qilu pharmaceutical (Hainan, China). IL-6, IL-1β and TNF-α ELISA kits were obtained from Jiangsu Meimian Industrial Co., Ltd. (Jiangsu, China).
3
Live/Dead Cell Staining Assay
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Live/dead staining assay was performed using a Calcein-AM/PI kit (CA1630, Solarbio, Beijing, China). B16-F10 cells were collected, and the supernatant was removed. Cell suspensions were prepared with a density of 1×105 cells/mL. Staining working solution (100 µL) was added to 200 µL cell suspensions, and the mixture was incubated at 37°C for 15 min. Then, the cells were observed under a confocal microscope (LEICA TCS SPE, Leica, Dresden, Germany).
4
Identifying and Targeting Cancer Stem Cells
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To detect the presence of CD133+ and CD44+ cells, anti-human/mouse CD44 (F1104401, 1 : 20, MULTISCIENCES, Hangzhou, China), fluorescein isothiocyanate (FITC) (MULTISCIENCES), and FITC anti-human CD133 antibody (567908, 1 : 20, BD Biosciences, Franklin Lakes, NJ, USA) were used to label the cells. Then, the cells were detected via flow cytometry (CytoFLEX, BECKMAN, Brea, CA, USA). Furthermore, CCSCs were treated with 1 μM oxaliplatin (#HY-17371, MedChemExpress, Monmouth Junction, NJ, USA) and 100 nM VD. A living/dead cell double staining assay was performed using the Calcein-AM/PI Kit (Solarbio, Beijing, China), according to the manufacturer's instructions. Dead cells were stained with PI, and their percentages were revealed when detected using flow cytometry.
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