Nbp243792

The following primary antibodies were used for immunoblotting and immunofluorescence: α-Actin (A5316, Sigma Aldrich), α-ATG3 (3415, CST), α-ATG101 (SAB4200175, Sigma-Aldrich), α-ATG13 (M183-3, MBL; SAB4200100, Sigma-Aldrich), α-ATG14 (PD026, MBL), α-ATG14 pS29 (92340, CST), α-Coilin (PA5-29531, Invitrogen), α-FIP200 (A301-574A, Bethyl), α-GAPDH (ab8245, Abcam), α-GFP (3H9, Chromotek), α-LC3B (2775, CST), α-p62 (GP62-C, PROGEN), α-pICln (sc-393525, Santa Cruz), α-PRMT5 (2252, CST), α-SmB (S0698, Sigma-Aldrich), α-SmD1 (ab79977, Abcam), α-SmD2 (SAB2102257, Sigma-Aldrich), α-SmE (NBP2-43792, Novus), α-SmF (SAB2102258, Sigma-Aldrich), α-SmG (HPA064152, Sigma-Aldrich), α-SMN (clone 2B1, 05-1532, Merck Millipore), α-SNRPB (Y12, MA5-13449, Invitrogen), α-Tubulin (clone B512, T5168, Sigma Aldrich), α-ULK1 (8054; CST), α-ULK2 (ab97695, Abcam), α-WD45 (2823, CST). The detection of proteins was carried out with the following fluorescent secondary antibodies: IRDye 680LT goat α-rabbit, IRDye 680LT goat α-mouse, IRDye 800CW donkey α-rabbit, IRDye 800CW donkey α-mouse, IRDye 800CW goat α-rat. For the detection of proteins in vivo via IF the following secondary antibodies were used: Alexa Fluor 568 donkey anti-mouse (A10037, Invitrogen) and Alexa Fluor 647 donkey anti-rabbit (A31573, Invitrogen).