Proteinase K and Buffer RLT lysis buffer were purchased from QIAgen (Germantown, MD, USA). TaqPath 1-Step RT-qPCR Master Mix, CG, UltraPure guanidine isothiocyanate, Gibco Beef Extract Powder, RNA-grade glycogen, and THE RNA Storage Solution were bought from ThermoFisher Scientific (Carlsbad, CA, USA). Polyethylene glycol (PEG) Bio Ultra 8000, glycine (electrophoresis grade), magnesium chloride hexahydrate, and the Proteinase K Inhibitor (tetrapeptidyl chloromethyl ketone) were bought from MilliporeSigma (Oakville, Ontario, Canada). SPRIselect magnetic beads were bought from Beckman Coulter (Brea, CA, USA). 2-Mercaptoethanol (2-ME), biotechnology grade, was bought from BioShop Canada (Burlington, Ontario, Canada). RNasin Plus Rnase Inhibitor was bought from Promega (Madison, WI, USA). Pseudovirus (SARS-CoV-2 RNA targets in a noninfectious viral coat) solution, AccuPlex SARS-CoV-2 Verification Panel-Full Genome, was bought from Sera Care, LGC (Milford, MA, USA). Purified SARS-CoV-2 RNA was provided by our colleagues in the Department of Medical Microbiology and Immunology at the University of Alberta. CDC N1 and N2 primer-probes 2019-nCoV RUO kit was bought from Integrated DNA Technologies (Coralville, IA, USA).
Ultrapure guanidine isothiocyanate
Manufactured by Thermo Fisher Scientific
Sourced in United States
UltraPure guanidine isothiocyanate is a chemical compound commonly used in molecular biology and biochemistry laboratories. It is a powerful chaotropic agent that effectively disrupts the hydrogen bonds in nucleic acids and proteins, facilitating their extraction and purification.
Automatically generated - may contain errors
Lab products found in correlation
Cdc n1 and n2 primer probes 2019 ncov ruo kit, by Integrated DNA Technologies (2 mentions)
Buffer rlt lysis buffer, by Qiagen (2 mentions)
Rna grade glycogen, by Thermo Fisher Scientific (2 mentions)
Rnasin plus rnase inhibitor, by Promega (2 mentions)
Rna storage solution, by Thermo Fisher Scientific (2 mentions)
Proteinase k, by Qiagen (2 mentions)
Taqpath 1 step rt qpcr master mix, by Thermo Fisher Scientific (2 mentions)
Spriselect magnetic beads, by Beckman Coulter (2 mentions)
Proteinase k inhibitor, by Merck Group (2 mentions)
Polyethylene glycol peg bio ultra 8000, by Merck Group (2 mentions)
Qiaamp viral rna mini kit, by Qiagen (1 mentions)
2 protocols using ultrapure guanidine isothiocyanate
1
SARS-CoV-2 RNA Detection Workflow
2
SARS-CoV-2 Detection Workflow Using RT-qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proteinase K, QIAamp Viral RNA
Mini Kit, and Buffer RLT lysis buffer were purchased from QIAgen (Germantown,
MD, USA). TaqPath 1-Step RT-qPCR Master Mix, CG, UltraPure guanidine
isothiocyanate, RNA-grade glycogen, and THE RNA Storage Solution were
bought from ThermoFisher Scientific (Carlsbad, CA, USA). CDC N1 and
N2 primer-probes 2019-nCoV RUO kit was bought from Integrated DNA
Technologies (Coralville, IA, USA). Proteinase K Inhibitor (tetrapeptidyl
chloromethyl ketone) was bought from MilliporeSigma (Oakville, Ontario,
Canada). RNasin Plus RNase Inhibitor was bought from Promega (Madison,
WI, USA). SPRIselect magnetic beads were bought from Beckman Coulter
(Brea, CA, USA). Silica-coated TurboBeads were ordered from TurboBeadsBio
(Zurich, Switzerland). Polyethylene glycol (PEG) Bio Ultra 8000 was
bought from MilliporeSigma. 2-Mercaptoethanol (2-ME), biotechnology
grade, was bought from BioShop Canada (Burlington, Ontario, Canada).
Pseudo-virus (SARS-CoV-2 RNA targets in a noninfectious viral coat)
solution, AccuPlex SARS-CoV-2 Verification Panel-Full Genome, was
bought from Sera Care, LGC (Milford, MA, USA). QuickExtract RNA Extraction
Kit and QuickExtract Plant DNA Extraction Solution were bought from
Lucigen (Middleton, WI, USA). Purified SARS-CoV-2 RNA was provided
by our colleagues in the Department of Medical Microbiology and Immunology
at the University of Alberta.
Mini Kit, and Buffer RLT lysis buffer were purchased from QIAgen (Germantown,
MD, USA). TaqPath 1-Step RT-qPCR Master Mix, CG, UltraPure guanidine
isothiocyanate, RNA-grade glycogen, and THE RNA Storage Solution were
bought from ThermoFisher Scientific (Carlsbad, CA, USA). CDC N1 and
N2 primer-probes 2019-nCoV RUO kit was bought from Integrated DNA
Technologies (Coralville, IA, USA). Proteinase K Inhibitor (tetrapeptidyl
chloromethyl ketone) was bought from MilliporeSigma (Oakville, Ontario,
Canada). RNasin Plus RNase Inhibitor was bought from Promega (Madison,
WI, USA). SPRIselect magnetic beads were bought from Beckman Coulter
(Brea, CA, USA). Silica-coated TurboBeads were ordered from TurboBeadsBio
(Zurich, Switzerland). Polyethylene glycol (PEG) Bio Ultra 8000 was
bought from MilliporeSigma. 2-Mercaptoethanol (2-ME), biotechnology
grade, was bought from BioShop Canada (Burlington, Ontario, Canada).
Pseudo-virus (SARS-CoV-2 RNA targets in a noninfectious viral coat)
solution, AccuPlex SARS-CoV-2 Verification Panel-Full Genome, was
bought from Sera Care, LGC (Milford, MA, USA). QuickExtract RNA Extraction
Kit and QuickExtract Plant DNA Extraction Solution were bought from
Lucigen (Middleton, WI, USA). Purified SARS-CoV-2 RNA was provided
by our colleagues in the Department of Medical Microbiology and Immunology
at the University of Alberta.
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