Dylight 488 goat anti rabbit igg

Manufactured by EarthOx

Sourced in United States

Dylight 488 goat anti-rabbit IgG is a secondary antibody used for immunofluorescence detection. It is conjugated with the DyLight 488 fluorescent dye, which has an excitation wavelength of 493 nm and an emission wavelength of 518 nm.

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Lab products found in correlation

Anti yap, by Cell Signaling Technology (1 mentions) Anti β catenin, by BD (1 mentions) Hrp conjugated goat anti mouse igg, by Bioworld Technology (1 mentions) Hrp conjugated goat anti rabbit igg, by Bioworld Technology (1 mentions) Anti becn1, by ABclonal (1 mentions) Anti atg5, by ABclonal (1 mentions) Anti caspase 3, by ABclonal (1 mentions) Anti parp, by Beyotime (1 mentions) Anti p62 sqstm1, by Merck Group (1 mentions) Anti caspase 8, by Beyotime (1 mentions) Anti caspase 9, by Beyotime (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions) Anti gapdh, by Beyotime (1 mentions) Dylight 488 goat anti mouse igg, by EarthOx (1 mentions) Ix81 confocal fluorescence microscopy, by Olympus (1 mentions) Fv1000 viewer ver 3.0a software, by Olympus (1 mentions)

4 protocols using dylight 488 goat anti rabbit igg

Immunofluorescence Staining of β-catenin and YAP

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Cells or frozen sections at 5 μm thickness were fixed in 4% paraformaldehyde and
methanol for 20 min at room temperature, respectively. Immunofluorescence was
performed as described previously.^{33 (link)} Antibodies used were including anti-β-catenin (1:1000, BD, 610154),
anti-YAP (1:500, Cell Signaling Technology, NO.14074), Dylight 649-goat
anti-mouse IgG (1:500, Earth Ox Life Sciences, E032610-01), and Dylight 488-goat
anti-rabbit IgG (1:500, EarthOx Life Sciences, E032220-01).

Wang S., Ma K., Zhou C., Wang Y., Hu G., Chen L., Li Z., Hu C., Xu Q., Zhu H., Liu M, & Xu N. (2019). LKB1 and YAP phosphorylation play important roles in Celastrol-induced β-catenin degradation in colorectal cancer. Therapeutic Advances in Medical Oncology, 11, 1758835919843736.

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Antibody Utilization for Apoptosis and Autophagy Detection

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The following primary antibodies were used in the study: rabbit polyclonal anti-LC3B (Cell Signaling Technology, 2775), rabbit polyclonal anti-SQSTM1/p62 (Sigma, SAB2104334), rabbit polyclonal anti-BECN1 (ABclonal, A7353), rabbit polyclonal anti-ATG5 (ABclonal, A0203), mouse monoclonal anti-GAPDH (Beyotime, AG019), rabbit polyclonal anti-caspase-8 (Beyotime, AC056), rabbit polyclonal anti-caspase-9 (Beyotime, AC062), rabbit polyclonal anti-Caspase-3 (ABclonal, A2156), rabbit polyclonal anti-PARP (Beyotime, AP102) and mouse monoclonal anti-CSFV E2 (WH303) (JBT, 9011). Mouse polyclonal anti-CSFV Npro was kindly provided by Dr. Xinglong Yu (Veterinary Department, Hunan Agricultural University, China). The secondary antibodies used for immunoblotting analysis were HRP-conjugated goat anti-mouse IgG (Bioworld, BS12478), HRP-conjugated goat anti-rabbit IgG (Bioworld, BS13278). The secondary antibodies used for immunofluorescence including Dylight 488 goat anti-mouse IgG (EarthOx, E032210), Dylight 488 goat anti-rabbit IgG (EarthOx, E032220), Dylight 549 goat anti-mouse IgG (EarthOx, E032310) and Dylight 549 goat anti-rabbit IgG (EarthOx, E032320).

Gou H., Zhao M., Fan S., Yuan J., Liao J., He W., Xu H, & Chen J. (2017). Autophagy induces apoptosis and death of T lymphocytes in the spleen of pigs infected with CSFV. Scientific Reports, 7, 13577.

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Colocalization of wild-type and mutant AQP0

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Wild-type and mutant AQP0 with FLAG tag were transfected separately into HEK 293T cells. For co-localization studies, FLAG-WT-AQP0 and Myc-Y219*-AQP0 constructs were co-transfected. Immunofluorescence studies were performed as described by Varadaraj et al [20 (link)]. Briefly, 24 h after transfection, cells cultured on glass coverslips were fixed in buffer containing 4% paraformaldehyde, and then counterstained with the nucleus-staining dye, DAPI. HEK 293T cells were subjected to immunofluorescence staining using c-Myc (9E 10) mouse monoclonal IgG (Santa Cruz Biotechnology, Shanghai, China) and rabbit anti-FLAG IgG(MEDICAL & BIOLOGICAL LABORATORIES CO.,LTD., Beijing, China), followed by Dylight 594 goat anti-mouse IgG and Dylight 488 goat anti-rabbitIgG(Earthox, San Francisco, CA,USA). All samples were analyzed by Olympus IX81 confocal fluorescence microscopy. Images were digitized and merged using FV1000 Viewer (Ver.3.0a) software (Olympus). Cells expressing the pCMV-3Tag-6or pCMV-3Tag-7 expression plasmid were used as negative controls. The assay was repeated three times.

Song Z., Wang L., Liu Y, & Xiao W. (2015). A Novel Nonsense Mutation in the MIP Gene Linked to Congenital Posterior Polar Cataracts in a Chinese Family. PLoS ONE, 10(3), e0119296.

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Immunofluorescence Analysis of Hypoxia Response

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After treatment with 1%O₂ or 21% O₂ for 24 h, HFFs were cultured on sterile glass slides in 6 cm culture dishes. After three washes with PBS, the cells were fixed with 4% formaldehyde for 15 min and permeabilized with 0.1% TritonX-100. The cells fixed on glass slides were blocked with 5% BSA and then incubated overnight with primary antibodies against HIF-1α, Smad2/3, Smad4, TβRII (as above for Western blotting, 1:200 dilution) at 4°C overnight. After three washes with PBS, the glass slides were incubated with Dylight 488 goat anti-rabbit IgG (E032220-01, EarthOx) for 1 h at room temperature. Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI). A fluorescence microscope was used to acquire cells images and observe protein localization.

Mingyuan X., Qianqian P., Shengquan X., Chenyi Y., Rui L., Yichen S, & Jinghong X. (2017). Hypoxia-inducible factor-1α activates transforming growth factor-β1/Smad signaling and increases collagen deposition in dermal fibroblasts. Oncotarget, 9(3), 3188-3197.

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