Western blot assays were performed using whole liver lysates and nuclear or microsomal protein lysates from the liver samples. Antibodies against mouse (# NB100-2522) or human (# D8012) SIRT6 were purchased from Novus Biologicals (Centennial, CO, USA) and Cell Signaling (Danvers, MA, USA), respectively. Antibodies against mouse P53 (# ab26 and # ab131442), human P53 (# ab246550), and Tubulin (# ab4074) were purchased from Abcam (Cambridge, Cambridgeshire, UK). Antibodies against human P53 (# SC-126) or Histone (# SC-517576) were purchased from Santa Cruz (Dallas, TX, USA). Antibodies against CYP7A1 (# TA351400) or CYP8B1 (# TA313734) were purchased from Origene (Rockville, MD, USA). The antibody against calnexin (#NB100-1965) was purchased from Novus (Centennial, CO, USA). Peroxidase-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Primary antibodies were diluted at 1:1000 except for the Tubulin antibody, which was diluted at 1:5000.
Ta313734
Manufactured by OriGene
Sourced in United States
TA313734 is a lab equipment product offered by OriGene. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ta313734
1
Western Blot Analysis of Liver Proteins
2
Liver Protein Analysis using Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total liver lysates and nuclear or microsome extracts of liver samples were used for Western blotting. The antibody against tubulin (cat. #ab4074) was purchased from Abcam. Antibodies against CYP7A1 (cat. #TA351400) or CYP8B1 (cat. #TA313734) were purchased from Origene. Antibodies against acetyl-CoA carboxylase (ACC) (cat. #3662), cleaved caspase 3 (cat. #9661), total caspase 3 (cat. #9662), phospho-Smad2/3 (cat. #8828), or total Smad2/3 (cat. #5678) were purchased from Cell Signaling Technology (Boston, MA, USA). Antibodies against histone (cat. #sc-393358) or SREBP-1 (cat. #SC-8984) were purchased from Santa Cruz Biotechnology. The antibody against calnexin (NB100-1965) was purchased from Novus.
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