The K1, 3D7 (from MR4 and in-lab maintained and isolated clone A10), and Dd2 strain of P. falciparum were cultured with RPMI 1640 medium containing 25 mM HEPES and 0.3 g/L l -glutamine (Gibco, Life Technologies, Paisley, UK) [32 (link),33 (link)]. The medium was supplemented with 2.5 g AlbuMax (Sigma, Gillingham, UK) and sterile solutions of 2.5 mL hypoxanthine (Sigma, UK), 2.5 mL 40% glucose (Dextrose Anhydrous, Fisher Scientific, Loughborough, UK), and 0.5 mL gentamycin (Sigma, UK). Human erythrocytes served as host cells. Cultures were maintained at 37 °C under gas mixture of 5% CO2, 5% O2 in N2 (BOC Limited, Manchester, UK).
Albumax
Manufactured by Merck Group
Sourced in United Kingdom
AlbuMax is a laboratory equipment product manufactured by Merck Group. It is designed for the separation and purification of albumin from biological samples. The core function of AlbuMax is to facilitate the isolation and concentration of albumin, a key protein found in blood plasma, using advanced chromatographic techniques.
Automatically generated - may contain errors
Lab products found in correlation
Hypoxanthine, by Merck Group (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Gentamycin, by Merck Group (2 mentions)
Hepes, by Thermo Fisher Scientific (2 mentions)
Hypoxanthine 2, by Merck Group (1 mentions)
Rpmi 1640 medium powder, by Thermo Fisher Scientific (1 mentions)
Hepes, by Merck Group (1 mentions)
Gentamicin, by Merck Group (1 mentions)
4 protocols using albumax
1
In vitro Cultivation of Malaria Parasites
2
Culturing Chloroquine-Resistant P. falciparum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroquine, pyrimethamine and sulfadoxine-resistant K1 strain of P. falciparum was cultured with RPMI (Roswell Park Memorial Institute) 1640 media containing 25 mM (2-hydroxyethyl) piperazine-N’-(2-ethane-sulfonic acid) (HEPES) and 0.3 g/L L-glutamine (Gibco, Life Technologies, Renfrew, UK). The medium was supplemented with sterile filtered 2.5 g AlbuMax (Sigma-Aldrich, Saint Louis, MO, USA), 2.5 mL hypoxanthine (Sigma-Aldrich, Saint Louis, MO, USA), 2.5 mL 40% glucose (Dextrose Anhydrous, Fisher Scientific, UK) and 0.5 mL gentamycin (Sigma-Aldrich, Saint Louis, MO, USA). Human erythrocytes served as host cells. Cultures were maintained at 37 °C under the gas mixture of 5% CO2 and 5% O2 in N2 gas mixture (BOC Limited, Guildford, UK).
3
Culturing Plasmodium falciparum 3D7
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
P. falciparum 3D7 parasites were cultured on O+ human erythrocytes (4% hematocrit) in complete RPMI 1640 medium containing 10.44 g/L RPMI medium 1640 powder (Gibco), 5.96 g/L Hepes (Sigma), 2 g/L NaHCO3, 5 g/L Albumax (Sigma), 50 mg/L hypoxanthine II (Sigma), and 40 mg/L gentamicin in a 37 °C humidified incubator maintained with a gas mixture of 5% CO2, 5% O2, and 90% N2 according to Trager and Jensen (63 (link)).
4
Synchronous Culture of P. falciparum Parasites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RPMI 1640 medium containing 25 mM HEPES and 0.3 g/liter l -glutamine (Gibco; Life Technologies, UK) supplemented with 2.5 g sterile filtered AlbuMax (Sigma, UK), 2.5 ml hypoxanthine (Sigma, UK), 2.5 ml 40% glucose (anhydrous dextrose; Fisher Scientific, UK), and 0.5 ml gentamicin (Sigma, UK) was used for the culture of erythrocytic-stage strain K1 P. falciparum parasites (gifted by John Hyde, University of Manchester, United Kingdom; original source, Thai-K1 clone) under a 5% CO2, 5% O2, and 90% N2 gas mixture (BOC Limited, UK) at 37°C. All routine culture methods were consistent with those described in reference 52 (link).
O positive human blood (purchased from NHS Blood Bank, Manchester, UK) was used routinely to maintain the parasites. Continuous cultures were maintained at 5% hematocrit. Since synchronous parasite development is observed in natural hosts and this synchrony is lost quite rapidly in in vitro cultures, sorbitol was used to keep the parasites in tight synchrony. In brief, the parasitized blood pellet was resuspended 1:10 in 5% sorbitol (prepared in distilled water and filtered using a 0.22-μm-porosity Millipore filter), incubated at room temperature for 5 min, and then centrifuged at 3,000 rpm for 5 min. The supernatant was removed, and complete medium was used to wash the pellet 3 times before setting up a new culture.
O positive human blood (purchased from NHS Blood Bank, Manchester, UK) was used routinely to maintain the parasites. Continuous cultures were maintained at 5% hematocrit. Since synchronous parasite development is observed in natural hosts and this synchrony is lost quite rapidly in in vitro cultures, sorbitol was used to keep the parasites in tight synchrony. In brief, the parasitized blood pellet was resuspended 1:10 in 5% sorbitol (prepared in distilled water and filtered using a 0.22-μm-porosity Millipore filter), incubated at room temperature for 5 min, and then centrifuged at 3,000 rpm for 5 min. The supernatant was removed, and complete medium was used to wash the pellet 3 times before setting up a new culture.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!