Whole cell lysates were prepared in NP-40 lysis buffer and immunoblotted with (i) anti-MUC1-C (ThermoFisher Scientific, Waltham, MA, USA; Cat. #HM-1630-P1, 1:50 dilution) and an anti-Armenian hamster secondary antibody (Abcam, Cambridge, MA, USA; Cat. #ab5745, 1:300 dilution), (ii) anti-PD-L1 (Cell Signaling Technology, Danvers, MA, USA; Cat. #13684S, 1:1000 dilution) and an anti-rabbit secondary antibody (GE Healthcare Life Sciences, Marlborough, MA, USA; Cat. #NA934V, 1:3000 dilution) and anti-β-actin (Sigma; Cat. A5316, 1:1000 dilution) and an anti-mouse secondary antibody (GE Healthcare Life Sciences; Cat. #NA931, 1:3000 dilution). Horseradish peroxidase secondary antibodies and enhanced chemiluminescence (GE Healthcare Life Sciences) were used for the detection of immune complexes. Immunoblot results were each confirmed with a second analysis.
Horseradish peroxidase secondary antibodies and enhanced chemiluminescence
Manufactured by GE Healthcare
Horseradish peroxidase (HRP) secondary antibodies and enhanced chemiluminescence are laboratory tools used for protein detection and analysis. HRP secondary antibodies bind to primary antibodies and catalyze a chemiluminescent reaction when combined with an appropriate substrate, allowing for the visualization and quantification of target proteins. This technique is commonly used in Western blotting, ELISA, and other immunoassays.
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Lab products found in correlation
2 protocols using horseradish peroxidase secondary antibodies and enhanced chemiluminescence
1
Western Blot Analysis of MUC1-C and PD-L1
2
Western Blot Analysis of MUC1-C and PD-L1
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