Purified bovine thrombin, recombinant thrombospondin 1 (TSP1) and SB 431542 hydrate were obtained from Sigma-Aldrich (Buchs, Switzerland). Highly purified full-length free human vWF (#V2651) and thrombin receptor activator peptide 6 (TRAP-6) were obtained from United States Biological (Salem, MA, USA). Recombinant human TGF-β1 and Interleukin-1β1 were obtained from PreproTech (Rocky Hill, NJ, USA). Prostaglandin I2 was purchased from Cayman chemical (Ann Arbor, MI, USA).
Purified bovine thrombin
Manufactured by Merck Group
Sourced in Switzerland, Germany
Purified bovine thrombin is a laboratory reagent derived from bovine blood. It is a serine protease enzyme that plays a key role in the coagulation cascade, catalyzing the conversion of fibrinogen to fibrin during blood clot formation.
Automatically generated - may contain errors
2 protocols using purified bovine thrombin
1
Thrombin and Thrombospondin Regulation of Cell Signaling
2
Hydrogel and Activated PRP for Wound Healing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Purified bovine thrombin (Sigma-Aldrich, Munich, Germany) was diluted at 100 units per mL in 10% calcium chloride and was then coagulated and activated by mixing with PRP. The hydrogel that was used in this experiment was Purilon gel (Coloplast, Humlebæk, Denmark), which has an amorphous property. For the hydrogel-only group, a 0.1-mL saline solution was injected into the base of each wound with a 1-mL syringe and a 27-gauge needle in place of PRP. In the PRP-only and combined-treatment groups, 0.1 mL of activated PRP was injected into the base of each wound using a 1-mL syringe and a 27-gauge needle. For the wound on the tail side, about 1 mL of hydrogel was applied locally. Each wound was dressed using Opsite (Smith & Nephew, London, UK), and the dressing was fixed with Surgifix (Panamedic). Each mouse was placed in a separate cage and observed for 2 weeks.
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