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2 protocols using bisoprolol hemifumarate salt

1

HPLC Method for Propranolol Quantification

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Reference standards of propranolol hydrochloride (≥98.5% purity), 4-hydroxypropranolol hydrochloride (≥98.5% purity), N-desisopropylpropranolol (≥97.5% purity) and bisoprolol hemifumarate salt (≥98% purity, Internal standard, IS) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade acetonitrile and methanol were obtained from Fisher Scientific (Pittsburgh, USA). All other chemicals were of analytical grade. Distilled water was purchased from Watsons (Hong Kong, China).
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2

LC-MS Quantification of Cardiovascular Drugs

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Reference drug samples: atenolol (R-(+), 99%), atenolol d 7 , atorvastatin calcium salt, bisoprolol hemifumarate salt, diltiazem hydrochloride, doxazosin mesylate salt, lisinopril, losartan potassium salt, ramipril, simvastatin and valsartan were purchased from Sigma-Aldrich (Poole, UK). LC-MS grade acetonitrile, methanol and water were also obtained from Sigma-Aldrich (Poole, UK). 903 specimen collection paper, polyethylene bags, microcentrifuge tubes (1.5 ml), pipette tips and volumetric pipettes were purchased from Fisher Scientific (Loughborough, UK). Autosampler vials with 250µl inserts, vial caps and formic acid were obtained from Agilent Technologies (Cheshire, UK). Heparin coated blood collection tubes were purchased from International Scientifique Supplies Ltd. (Bradford, UK). An 8 mm diameter punch was acquired from Maun Industries Ltd. (Nottingham, UK).
Following De Montfort University's Ethics Protocols, fresh blank blood was obtained from informed volunteers.
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