10 mg tissues of kidney were sliced and rinsed with cold saline in each group. Immediately homogenize the tissue by shaking the homogenizer with cold saline. Otherwise, the ultrasonic cell disrupter homogenized the cells. To detect the MDA concentrations of tissues and cells,the supernatant was collected after centrifugation for 10 min. The concentrations of MDA were measured by Micro Malondialdehyde Assay Kit (Solarbio, Beijing, China). Then transfer the working reagent and collect the supernatant into a 96-well plate. To calculate the MDA concentration,the optical density was detected at the wavelength of 600, 532 and 450 nm. After tissues of kidney were homogenized, the supernatant was collected after centrifugation. In addition, the ultrasonic cell disrupter homogenized the cells. 4-HNE concentrations was measured by 4-HNE ELISA Kit (Fankewei, Shanghai, China). Microplate reader detected the optical density of the supernatant at the wavelength of 450 nm. And 4-HNE concentrations of the supernatant were then obtained by comparing the optical density of the samples to the standard curve.
Micro malondialdehyde assay kit
Manufactured by Solarbio
Sourced in China
The Micro Malondialdehyde Assay Kit is a laboratory tool used to measure the level of malondialdehyde, a biomarker for oxidative stress. The kit provides reagents and protocols to quantify malondialdehyde concentrations in small sample volumes.
Automatically generated - may contain errors
Lab products found in correlation
Gsh assay kit, by Beyotime (1 mentions)
Catalase assay kit, by Nanjing Jiancheng (1 mentions)
Glutathione assay kit, by Nanjing Jiancheng (1 mentions)
Total antioxidant capacity assay kit, by Nanjing Jiancheng (1 mentions)
Micro reduced glutathione assay kit, by Solarbio (1 mentions)
Facsverse, by BD (1 mentions)
Ferroorange, by Dojindo Laboratories (1 mentions)
Iron detection kit, by Leagene (1 mentions)
7 protocols using micro malondialdehyde assay kit
1
Kidney Tissue Malondialdehyde and 4-HNE Quantification
2
Kidney Tissue Biochemical Analysis
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10 mg kidney tissue was taken from each group and rinsed with saline. The tissue was homogenized and immediately mixed with cold salt water. Then the cells were collected, homogenized with an ultrasonic cell pulverizer, and centrifuged for 10 min to collect the supernatant. Similarly, the cells were collected and homogenized with ultrasonic cell pulverizer, and centrifuged for 10 min to collect the supernatant. Subsequently, an iron assay kit, a micro malondialdehyde assay kit (Solarbio, Beijing, China), and a GSH assay kit (Beyotime, Nanjing, China) were used to assess the iron level, MDA activity, and GSH levels, respectively, according to a previous study19 .
3
Antioxidant Status Measurement Protocol
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The serum malondialdehyde (MDA ) was determined by Micro Malondialdehyde Assay Kit (BC0025; Solarbio Technology Co. Ltd, Beijing, China). The serum superoxide dismutase (SOD ) was determined by Superoxide Dismutase Assay Kit (SOD-1-Y; Comin Biotechnology Co. Ltd, Suzhou, China). The serum GSH, catalase (CAT ), and total antioxidant capacity (T-AOC ) were determined by Glutathione Assay Kit (A006-2-1), Catalase Assay Kit (A007-1), and Total Antioxidant Capacity Assay Kit (A015-2-1) from Jiancheng Bioengineering Institute Co., Ltd (Nanjing, China).
4
Quantifying Oxidative Stress Markers
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MDA was detected by a Micro Malondialdehyde Assay Kit (Solarbio, BC0025, China). Cys was detected by a Micro Cysteine Assay Kit (Solarbio, BC0185, China). GSH was detected by a Micro Reduced Glutathione Assay Kit (Solarbio, BC1175, China).
5
Quantifying Oxidative Stress in Autoimmune Disorders
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The MDA concentrations in skin of IMQ-induced psoriasis-like mice and spleens and dLNs of MRL/lpr mice were detected with the Micro Malondialdehyde Assay Kit (Beijing Solarbio Science and Technology Co.) following the manufacturer’s instructions.
6
Micro Malondialdehyde Assay Protocol
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The content of MDA was examined using a micro malondialdehyde assay kit (Solarbio, Beijing, China). After the sample was collected, all operations were carried out according to the manufacturer’s instruction. Each experiment was performed in three replicates.
7
Intracellular Iron and Oxidative Stress
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Intracellular ferrous iron (Fe 2+ ) was assessed by uorescent probe FerroOrange (F374; Dojindo) via uorescence-activated cell sorter (FACS) analysis using BD FACSVerse (BD biosciences, NJ, USA), while total iron concentration was measured by Iron detection kit (TC1015; Leagene, Bejing, China). MDA concentrations were determined by the Micro Malondialdehyde Assay Kit (BC0025; Solarbio, Beijing, China).
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