Anti fas jo2

Manufactured by BD

Sourced in United States

The Anti-Fas Jo2 is a laboratory equipment product manufactured by BD. It is a reagent that binds to the Fas receptor, which is involved in the regulation of apoptosis, or programmed cell death. The core function of this product is to facilitate the study of Fas-mediated cell signaling pathways.

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Lab products found in correlation

Rat igg2a isotype control 2a3, by BioXCell (1 mentions) Rat igg2b isotype control ltf 2, by BioXCell (1 mentions) Brdu flow kit, by BD (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti b220 ra3 6b2, by BD (1 mentions) Anti igd 11 26c 2a, by BD (1 mentions) Anti cd45.1 a20, by BD (1 mentions) Anti cd45.2 104, by BD (1 mentions) Anti cd69 h1.2f3, by Thermo Fisher Scientific (1 mentions) Anti gl7 gl 7, by Thermo Fisher Scientific (1 mentions) Anti il 4 11b11, by BioLegend (1 mentions) Moma 1, by Abcam (1 mentions) Anti bcl6 k112 91, by BD (1 mentions) Anti cxcr5 2g8, by BD (1 mentions) Fitc dextran, by Merck Group (1 mentions) Anti gfp antibody, by Abcam (1 mentions) Np2 bsa, by Biosearch Technologies (1 mentions) Anti igd 11 26c 2a, by BioLegend (1 mentions) Np osu, by Biosearch Technologies (1 mentions) Np pe, by Biosearch Technologies (1 mentions)

Spelling variants of this product

Fas (Jo2) (10 citations) Anti-Fas (6 citations) Anti-Fas (clone Jo2, (3 citations) Phycoerythrin-conjugated anti-Fas/CD95 (clone Jo2) (2 citations)

5 protocols using anti fas jo2

Multiparameter Flow Cytometry Analysis of Germinal Center B Cells

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Spleen and lymph nodes were isolated and mashed into media containing
2% FCS. For analysis of GC B cells, cells were stained with anti-B220
(RA3-6B2; BD Biosciences or Biolegend); anti-CD19 (6D5; BD Biosciences);
anti-Fas (Jo2; BD Biosciences); anti-IgD (11-26c.2a; BD Biosciences or
Biolegend); anti-CD45.1 (A20; BD Biosciences or Biolegend); anti-CD45.2 (104; BD
Biosciences or Biolegend); anti-IgG2b (RMG2b-1; BD Biosciences); homemade
Alexa647 conjugated DEL; antibody to T cell and B cell activation antigen (GL7;
BD Biosciences); anti-Mouse Eα52-68 peptide bound to I-A^b(Y-Ae, eBioscience); anti-integrin β1 (MB1.2; Chemicon); anti-integrin
β2 (C71/16; BD Biosciences); anti-integrin β3 (2C9.G2;
Biolegend); anti-integrin β7 (M293; BD Biosciences); anti-integrin
α4 (PS/2; Bio X Cell); anti-integrin α_L (M17/4; Bio X
Cell); anti-integrin α_V (RMV-7; BD Biosciences); rat IgG2a
isotype control (2A3; Bio X Cell); rat IgG2b isotype control (LTF-2; Bio X Cell)
or rat IgG1 isotype control (R3-34; BD Biosciences). BrdU staining was done
using BrdU flow kit (BD Biosciences) following manufacturer's
instructions. For intracellular staining of phosphorylated AKT at Ser473 (pAKT),
cells were instantly fixed and stained as described (35 (link)). Anti-pAKT (9271; Cell Signaling Technology) was
used.

Wang X., Rodda L., Bannard O, & Cyster J.G. (2014). Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4601-4609.

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Comprehensive Immune Cell Staining

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The following staining reagents were used: anti-CD3 (145-2C11), anti-CD4 (H129.19), anti-CD62L (MEL-14), anti-CD44 (IM7), anti-CD45R (RA3-6B2), anti-PD-1 (29F.1A12), anti-PD-L1 (10F.9G2), anti-ICOSL (HK5.3), anti-CD40 (HM40-3), anti-CD40L (MR1), anti-CXCR4 (L276F12), and anti-IL-4 (11b11) were from Biolegend (San Diego, CA, USA); anti-CD19 (eBio1D3), anti-ICOS (7E.17G9), anti-IL-21 (mhalx21), anti-CD69 (H1.2F3), and anti-GL7 (GL-7) were from eBiosciences (San Diego, CA, USA); anti-Bcl-6 (K112-91), anti-CXCR5 (2G8), and anti-Fas (Jo2) were from BD Biosciences (Franklin Lakes, NJ, USA). PNA (B-1075; Vector Laboratories, Burlington, ON, Canada) was used for staining germinal center B cells and MOMA-1 (Abcam, Cambridge, UK) for staining MZ macrophages.

Daugan M., Murira A., Mindt B.C., Germain A., Tarrab E., Lapierre P., Fritz J.H, & Lamarre A. (2016). Type I Interferon Impairs Specific Antibody Responses Early during Establishment of LCMV Infection. Frontiers in Immunology, 7, 564.

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Murine B Cell Phenotyping and Detection

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Fluorophore-labeled anti-B220 (RA3-6B2) and anti-FAS (Jo2) antibodies were from BD; anti-CD138 (281-2), anti-CD21/35 (CR2/CR1), and anti-IgD (11-26c2a) antibodies were from BioLegend; anti-GL7 (GL7), anti-CD38 (90), and anti-rabbit IgG antibodies were from Invitrogen; rabbit anti-cCasp3 and the matching isotype control antibodies were from Cell Signaling; and anti-GFP antibody was from Abcam. The fixable viability dye, zombie yellow, was from BioLegend. NP₁₈-OVA, NP₃₀-Ficoll, NP₂-BSA, NP-PE, and 4-hydroxy-3-iodo-5-nitrophenylacetyl (NIP)-BSA-biotin were from Biosearch Technologies. NP-APC conjugates were made by allowing NP-Osu (Biosearch Technologies) and APC (BioLegend) to react for 4 h at room temperature in a buffer containing 0.1 M NaHCO₃ and 0.15 M NaCl₂ (pH 8), at a molar ratio of 20:1. Dextran (200 kD, 31398) and Dextran-FITC (2,000 kD, FD2000S) were from Sigma-Aldrich.

Liu X., Zhao Y, & Qi H. (2022). T-independent antigen induces humoral memory through germinal centers. The Journal of Experimental Medicine, 219(3), e20210527.

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Modulating Cell Death Pathways

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In HepG2 cells or PHHs, Fas receptor stimulation was performed with 10 ng/mL of super-hFasL (ALX-522-020, Enzo Life Sciences Inc., Farmingdale, NY). DR4/DR5 receptor stimulation was performed with 1 mg/mL of active recombinant human soluble TRAIL (catalog number 310-04, PeproTech, Rocky Hill, NJ). In PMHs, Fas receptor stimulation was performed with the agonistic monoclonal antibody anti-Fas Jo2 (catalog number 554255, BD Pharmingen) alone at 2 mg/mL. To enhance FasL-induced apoptosis, cells were pretreated with the 60 mg/mL of the p53 inducer bleomycin (Sigma-Aldrich) or 0.2 mg/mL of doxorubicin (Sigma-Aldrich). To neutralize TRAIL-induced apoptosis, cells were pretreated with 10 mg/mL of DR4 (HS101, 10 P's BVBA, Breda, the Netherlands) and/or DR5 blocking antibody (HS201, 10 P's BVBA, Breda, the Netherlands).

Liu W., Guo T.F., Jing Z.T, & Tong Q.Y. (2019). Repression of Death Receptor-Mediated Apoptosis of Hepatocytes by Hepatitis B Virus e Antigen. The American journal of pathology, 189(11).

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Modulation of Fas-Mediated Apoptosis in HepG2 and PMHs

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In HepG2 cells, Fas receptor stimulation was performed with the agonistic monoclonal antibody anti-Fas CH11 (catalog number SY-001; MBL, Nagoya, Japan) at 2 mg/mL plus 0.5 mg/mL actinomycin D (catalog number A1410; Sigma-Aldrich, St. Louis, MO). In PMHs, Fas receptor stimulation was performed with the agonistic monoclonal antibody anti-Fas Jo2 (catalog number 554255; BD Pharmingen, San Diego, CA) alone at 2 mg/mL. To oppose agonistic anti-Fas antibodyeinduced apoptosis, cells were pretreated with the Fas receptor antagonistic monoclonal antibody anti-Fas ZB4 (catalog number MD-11-3; MBL) at 2 mg/mL or AKT activator SC79 (catalog number 123871; Calbiochem, La Jolla, CA) at 4 mg/mL. To potentiate agonistic anti-Fas antibodyeinduced apoptosis, cells were pretreated with the AKT inhibitor LY294002 (catalog number L9908; Sigma-Aldrich) at 50 mmol/L. All of these pretreated cells were then treated with anti-Fas CH11 or Jo2 at 2 mg/mL for an additional 4 hours. IgM (catalog number M079-3; MBL) was used as a control for anti-Fas CH11, and IgG (catalog number M075-3; MBL), for anti-Fas ZB4.

Liu W., Jing Z.T., Wu S.X., He Y., Lin Y.T., Chen W.N., Lin X.J, & Lin X. (2018). A Novel AKT Activator, SC79, Prevents Acute Hepatic Failure Induced by Fas-Mediated Apoptosis of Hepatocytes. The American journal of pathology, 188(5).

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