The largest database of trusted experimental protocols

6520 quadrupole time of flight mass spectrometer

Manufactured by Agilent Technologies
Sourced in United States

The 6520 quadrupole time-of-flight mass spectrometer is a high-performance analytical instrument designed for accurate mass measurement and identification of chemical compounds. It combines a quadrupole mass analyzer with a time-of-flight mass analyzer to provide precise mass data.

Automatically generated - may contain errors

18 protocols using 6520 quadrupole time of flight mass spectrometer

1

Comprehensive LC-MS Analysis of Metabolic Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liquid chromatography and mass spectrometry (LC-MS) analysis were performed using an HPLC-ESI-QTOF-MS instrument on an Agilent 6520 quadrupole time-of-flight (QTOF) mass spectrometer connected with Agilent 1200-HPLC system via Dual electrospray ionization (ESI) interface (Agilent Technologies, USA). 1 mg/mL stock solution of methanol extract was filtered through a 0.22 μm syringe filter. The filtered stock solution was further diluted to 500 ppm using methanol. The prepared dilution was transferred into a high-performance liquid chromatography (HPLC) autosampler vial for LC-MS analysis and 1 μL aliquot was injected into the HPLC-ESI-QTOF-MS system. The Mass Hunter software version B.04.00 build 4.0.479.0 (Agilent Technology) was used to analyze chromatogram, mass spectra, exact mass calculation, and prediction of chemical formula of the identified compounds as by Singh et al. [38 (link)].
+ Open protocol
+ Expand
2

ALDH Isoenzyme Complex Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complexes for analysis by mass spectrometry were formed from 2.5–10.0 μM of the ALDH isoenzyme with 10 μM DEAB with or without 100–500 μM NAD+ and incubated for 1 h at room temperature in 10 mM HEPES, pH 7.5. Samples (0.5–5 μL) were injected using an Agilent 1200SL HPLC with a low rate of 0.3 mL/min consisting of 70% H2O and 30% acetonitrile with 0.1% formic acid into an Agilent 6520 quadrupole-time of flight (Q-TOF) mass spectrometer operating in TOF mode. The spectra were extracted and deconvoluted using MassHunter and Bioconfirm software.
+ Open protocol
+ Expand
3

DON Quantification by LCMS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Derivatized DON samples (2 μL) prepared as described above were injected and separated on an Agilent 1290 LC equipped with an Agilent Eclipse Plus 2.1 × 100 mm, 1.8 micron Rapid Resolution C18 column over a 5.5 minute gradient from 30–70% acetonitrile + 0.1% formic acid. Analytes were detected with an Agilent 6520 quadrupole time-of-flight (QTOF) mass spectrometer in positive mode with drying gas at 350 °C, 11 L/min and 40 psi. The fragmenter was set at 70V and the VCAP at 4000V.
+ Open protocol
+ Expand
4

Proteomic Analysis of Naja haje Venom

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteomic analysis of digested Naja haje fraction was carried out in an Agilent 1200 Series HPLC-Chip/MS system connected to a 6520 Quadrupole-Time of Flight (Q-TOF) mass spectrometer, equipped with a nano-electrospray source (Agilent Technologies, Santa Clara, CA, USA). For the online fractionation, two microliters of tryptic peptides were loaded and enriched on a 160 nL RP-C18 trap column, then separated on an analytical nano-column (150 mm × 75 µm) packed with ZORBAX SB-C18, 5 µm, 300Å (G4240-62010; Agilent Technologies). The separation was maintained over 25 min at 450 nL/min, using a linear gradient from 3 to 80% ACN in 0.1% FA. The eluted peptides were operated in positive mode in the Q-TOF mass spectrometer; the mass range was set from 290 to 1700 m/z and from 59 to 1700 for the MS and MS/MS scans, respectively. The total cycle time was two seconds. In each cycle, five of the most abundant precursor ions were subjected to MS/MS fragmentation; the ions with single charge were excluded, while the collision energy was automatically adjusted according to m/z [44 (link)].
+ Open protocol
+ Expand
5

Comprehensive 5S rRNA Modification Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The full spectrum of modified ribonucleosides in the purified 5S rRNA was characterized by analysis of ribonucleoside hydrolysates of the RNA by chromatography-coupled quadrupole time-of-flight and triple quadrupole mass spectrometry, using methods described previously (15 (link),26 (link),27 (link)). Briefly, purified BCG 5S rRNA was enzymatically hydrolyzed in the presence of antioxidants and deaminase inhibitors and the ribonucleosides resolved by HPLC using a Hypersil GOLD aQ Analytical HPLC Column (100 × 2.1 mm, 1.9-μm particle size) (Thermo Scientific, Wilmington, DE, USA) coupled to either an Agilent 6460 triple quadrupole mass spectrometer or an Agilent 6520 quadrupole time-of-flight mass spectrometer (LC-MS/MS) with ESI ionization operated in positive ion mode. The identity of individual ribonucleosides was confirmed by comparison with synthetic standards for HPLC retention time, exact molecular weight and collision-induced dissociation (CID) fragmentation patterns. In cases where a standard was not available, a putative structure was inferred from exact molecular weight and CID fragmentation patterns.
+ Open protocol
+ Expand
6

Antibiotic Production and Characterization of M. purpurea

Check if the same lab product or an alternative is used in the 5 most similar protocols
The fermentation process and metabolite extraction of M. purpurea was done based on previously described protocols [21 ]. Its antibiotic activity was detected by the cylinder-plate method, which was done according to Pharmacopoeia of the People’s Republic of China (2010 edition) [22 ]. The components of the product were analyzed by thin layer chromatography (TLC) using silica gel GF254 plate. The development solvent used in the assay was the underlayer of the mixture (chloroform: methanol: ammonia in 1:1:1 volume ratio). Accurate component ascertainment was performed by electrospray ionization mass spectrometry (ESI/MS) using Agilent 6520 quadrupole-time-of-flight mass spectrometer. The scanning range of Q-TOP-MS is set as following: Positive ion mode m/z 100~800; the dry gas is N2; the flow rate is 8mL/min; the temperature is 350 °C; atomization pressure is 2.07 × 105 Pa; capillary voltage is 3500 V; the fragmentation voltage is 135 V; and Agilent MassHunter is used to analysis MS data (B.04.00).
+ Open protocol
+ Expand
7

HPLC-QTOF-MS Analysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Online HPLC-QTOF-MS analysis was made using an Agilent 1290 infinity LC system connected to a 6520 quadrupole time-of-flight mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with an electrospray interface. The positive and negative ion ESI-MS experiments were conducted using conditions as follows: drying gas temperature, 320°C; drying gas (N2) flow rate, 10 l/min; nebulizer, 45 psi; capillary voltage, 4000 V for positive mode and 3500 V for negative mode; and fragmentor, 175 V. All the operation, acquisition, and analysis of data were made by Agilent Mass Hunter Acquisition Software Version B.04.00 (Agilent Technologies).
+ Open protocol
+ Expand
8

Targeted Metabolite Profiling by LC-QTOF-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liquid chromatography was performed on an Agilent Series G2226A pump by injecting 5 μL sample onto an Agilent Zorbax SB-Aq Rapid Resolution HT 2.1 x 100 mm, 1.8 μm, 600 bar analytical column, coupled to an Agilent Zorbax SB-Aq Narrow-Bore 2.1 x 12.5 mm, 5 μm guard column. The autosampler tray temperature was set at 4 °C and column compartment was set at 30 °C. Samples were run at a flow rate of 0.3 mL/min, using mobile phase A (water with 0.1% formic acid), and mobile phase B (90:10 acetonitrile:water with 0.1% formic acid). Gradient elution was as follows: 0-3 min 2% B, 3-5 min 2-40% B, 5-20 min 40-100% B, 20-30 min 100% B, followed by column re-equilibration. An Agilent 6520 quadrupole time-of-flight mass spectrometer (Q-TOF-MS) was used to analyze samples in positive and negative ionization mode at mass range 50-1700 m/z, scan rate 2.22 spectra/s, gas temperature 300oC, and gas flow 10 L/min. The nebulizer was 30 psi, skimmer 60 V, capillary voltage 4000 V, and fragmentor 120 V in positive mode and 140 V in negative mode, with reference masses 121.050873 and 922.009798 for positive mode and 112.985628 and 966.000725 for negative mode (Agilent reference mix).
+ Open protocol
+ Expand
9

Plasma Metabolite Profiling by GC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Amino acids were measured after purification and derivatization of 100-μL samples of plasma via gas chromatography–mass spectrometry (Agilent 6890N Gas Chromatograph coupled to 5973 MSD Mass Spectrometer) using a modified EZ:faast kit (Phenomenex); norvaline was used as an internal standard (16 (link),20 (link)). TCA metabolites were extracted from 100 μL plasma with a mixture of methanol, chloroform, and water (8:1:1) containing 13C isotope–labeled internal standards for citrate, succinate, fumarate, malate, α-ketoglutarate, lactate, and pyruvate. Liquid chromatography–mass spectrometry analysis was performed on an Agilent system consisting of a 1260 ultraperformance liquid chromatography module coupled with a 6520 quadrupole time-of-flight mass spectrometer (Agilent Technologies, Santa Clara, CA). Data were processed using MassHunter Quantitative Analysis, version B.07.00. Metabolites were normalized to the nearest isotope-labeled internal standard and quantitated using a linear calibration curves (16 (link)).
+ Open protocol
+ Expand
10

HILIC-QTOF-MS for Metabolite Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples (both in vivo and in vitro) were centrifuged at 14,000× g rpm for 10 min at 4 °C, and the supernatants were transferred to glass insert liquid chromatography vials. Analyses occurred on an Agilent 1290 liquid chromatography system coupled to an Agilent 6520 quadrupole time of flight mass spectrometer. Samples (5 µL) were injected and separated on a Waters Acquity UPLC BEH (bridged ethyl hybrid) Amide 1.7 µm 2.1 × 100 mm HILIC (hydrophilic interaction liquid chromatography) column with a flow rate of 0.3 mL/minute. Mobile phases consisted of A (water + 0.1% formic acid) and B (acetonitrile + 0.1% formic acid). The column was equilibrated at 2.5/97.5 (A/B) and maintained for 1 min post injection. Mobile-phase A increased in a linear gradient from 2.5% to 65% from 1 to 9 min post injection then stepped to 97.5% A from 9 to 11 min to wash the column. Column was equilibrated in starting condition for 3 min before the next injection. The mass spectrometer, equipped with a dual electrospray ionization source, was run in negative ion and then in positive ion mode. The scan range was 50–1600 m/z. The source settings consisted of drying gas flow rate: 11 L/min; nebulizer: 40 pounds per square inch gauge; gas temp: 350 °C; capillary voltage: 3000 V (neg), 2500 V (pos).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!