LPS, Apelin-13, F13A (a selective APJ receptor antagonist), DPI (an NAPDH oxidase inhibitor, diphenylene iodonium), NAC (a superoxide inhibitor, N-Acetyl Cysteine) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Mouse tumor necrosis factor (TNF)-α, IL-1β, IL-6 and Apelin-13 enzyme linked immunosorbent assay (ELISA) kits were purchased from Cusabio (Houston, TX, USA). The reactive oxygen species assay kit (DCF-DA) was from Applygen (Beijing, China). The H2O2 assay kit was from Beyotime (Shanghai, China). NOX4- small-interfering RNA (siRNA) and PFKFB3 siRNA were provided by GenePharma (Shang hai, China). Mouse PFKFB3 (NM_001177756) overexpressed plasmid was purchased from MiaoLing Plasmid Sharing Platform (Hubei, China). Other reagents are described below.
Apelin 13
Manufactured by Cusabio
Sourced in United States
Apelin-13 is a synthetic peptide that corresponds to the 13-amino acid sequence of the apelin protein. Apelin is a bioactive peptide that plays a role in various physiological processes, including cardiovascular function, fluid homeostasis, and energy metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Apelin 13, by Merck Group (1 mentions)
Enzyme linked immunosorbent assay elisa kit, by Cusabio (1 mentions)
H2o2 assay kit, by Beyotime (1 mentions)
Pfkfb3 sirna, by GenePharma (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Il 1β, by Cusabio (1 mentions)
Interleukin 6 (il 6), by Cusabio (1 mentions)
Gdf 15, by R&D Systems (1 mentions)
2 protocols using apelin 13
1
Apelin-13 Modulates Inflammatory Response
2
Apelins and GDF-15 Biomarker Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Serum samples were stored at room temperature for 30 min, then centrifuged at 1500 G for 10 min and aliquoted to 250 µl. All samples were stored at -80°C in the Biobank of the Medical University of Graz until analysis. For the determination of apelins and GDF-15, commercially available enzyme-linked immunosorbent assays (ELISA) were used (apelin-13: Cusabio Biotech Co., LTD., Cat. No. CSB-E13072h), (apelin-17: Antibodies online Cat. No. ABIN6953820 for the pilot study and USCN Life Science Inc., Cat. No. CED065Hu for the prospective study), (apelin-36: Cusabio Biotech Co., LTD., Cat. No. CSB-E13566h), (GDF-15: R&D, Cat. No. DGD150). According to the manufacturer, no significant cross-reactivity or interference was observed between apelin-17 and analogues. NT-proBNP was determined during routine testing at the hospital’s central laboratory (Roche, Cobas).
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