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Apc annexin 5 apoptosis detection kit

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The APC Annexin V Apoptosis Detection Kit is a laboratory instrument used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule that becomes exposed on the surface of cells during apoptosis. The APC (Allophycocyanin) fluorescent dye is conjugated to Annexin V, allowing for the detection and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Facscanto 2 flow cytometer, by BD (7 mentions) Facscanto 2, by BD (4 mentions) Facscan, by BD (3 mentions) Facscalibur, by BD (3 mentions) Facsdiva software, by BD (2 mentions) Annexin 5 binding buffer, by BioLegend (2 mentions) Flica 660 caspase 1 assay, by ImmunoChemistry Technologies (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Lsrii flow cytometer, by FlowJo (2 mentions) Fcs3 express software, by De Novo Software (2 mentions) 7 aad, by BioLegend (2 mentions) Accuri c6, by BD (2 mentions) Fortessa x20, by BD (2 mentions) Cold staining buffer, by BioLegend (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Mhcd4528, by BioLegend (1 mentions) Facsaria 2, by BD (1 mentions) Flojo software, by Tree Star (1 mentions) Facscanto 2 488n, by BD (1 mentions)

Spelling variants of this product

Annexin V (328 citations) Annexin V-APC (225 citations) APC Annexin V Apoptosis Detection Kit with PI (57 citations) Apoptosis detection kit (47 citations) Annexin V Apoptosis Detection Kit (30 citations) Annexin V APC/PI Apoptosis Detection Kit (8 citations) APC Annexin V kit (5 citations) Annexin V detection kit (5 citations) Annexin V kit (4 citations) APC Annexin V apoptosis kit (3 citations)

61 protocols using apc annexin 5 apoptosis detection kit

1

Annexin-V Apoptosis Detection in Lymphocytes

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Lymphocyte apoptosis was identified by flow cytometry analyses using an APC Annexin-V apoptosis detection kit (BioLegend, catalog no. 640930). First, the cells were stained for monoclonal antibodies. A cold staining buffer (BioLegend, catalog no. 420201) was used to wash the cells. The cells were then resuspended in an annexin-V binding buffer (BioLegend, catalog no. 422201). A total of 5 μL annexin-V (8 µg/mL) was added to each tube for fifteen minutes in the dark at normal temperatures. The annexin-V binding buffer was then added. The analysis of the cells was carried out utilizing FACSDiva software (BD Biosciences, San Diego, CA, USA). Data were shown as percentages of annexin-V+ lymphocytes. The positive threshold was constructed using non-stained controls.
, & Saber M.M. (2023). PD-L1 Is Involved in the Development of Non-Hodgkin’s Lymphoma by Mediating Circulating Lymphocyte Apoptosis. Vaccines, 11(9), 1474.
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2

Apoptosis and Caspase-1 Activity Assay

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AM apoptosis was assessed by Annexin V and 7-AAD staining using an apoptosis detection kit (APC Annexin V Apoptosis Detection kit, Biolegend) according to the manufacturer’s instructions. For detection of active caspase-1 activities in AMs, cells were incubated with active caspase-1 detection reagent (FLICA 660 Caspase-1 Assay, Immunochemistry Technologies) for 45 min at 37 °C as described in the manufacturer’s instructions.
Zhu B., Wu Y., Huang S., Zhang R., Son Y.M., Li C., Cheon I.S., Gao X., Wang M., Chen Y., Zhou X., Nguyen Q., Phan A.T., Behl S., Taketo M.M., Mack M., Shapiro V.S., Zeng H., Ebihara H., Mullon J.J., Edell E.S., Reisenauer J.S., Demirel N., Kern R.M., Chakraborty R., Cui W., Kaplan M.H., Zhou X., Goldrath A.W, & Sun J. (2021). Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection. Immunity, 54(6), 1200-1218.e9.
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3

Clonogenic Assay and Apoptosis Analysis

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For the clonogenic assay, cells were seeded into 60-mm culture dishes. After an incubation period of 2 weeks, the colonies were fixed with methanol and stained with crystal violet. Cell apoptosis was determined through APC Annexin V/Propidium Iodide (PI) assay staining by using the APC-Annexin V Apoptosis Detection Kit (BioLegend) according to the manufacturer's protocol. At specific time-points, cells were stained and analyzed by flow cytometry (FACS Canto II flow cytometer, BD Biosciences).
Wilson A., Menon V., Khan Z., Alam A., Litovchick L, & Yakovlev V. (2019). Nitric oxide-donor/PARP-inhibitor combination: A new approach for sensitization to ionizing radiation. Redox Biology, 24, 101169.
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4

Annexin V-Based Apoptosis Assay

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The apoptosis cells were measured with the Annexin V staining by APC Annexin V apoptosis detection kit with 7-AAD according to the manufacturer's (BioLegend, San Diego, CA). Annexin V positive but 7-AAD negative (early apoptotic cells) and Annexin V positive and 7-AAD positive (late stage apoptosis) was determined by Gallios flow cytometer (Beckman Coulter, Brea, CA, USA).
Ohira T., Kojima H., Kuroda Y., Aoki S., Inaoka D., Osaki M., Wanibuchi H., Okada F., Oshimura M, & Kugoh H. (2019). PITX1 protein interacts with ZCCHC10 to regulate hTERT mRNA transcription. PLoS ONE, 14(8), e0217605.
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5

Apoptosis Measurement in BMDMs

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BMDMs were transferred to serum-free medium (SFM) for 30 min and then treated with S-PrP (40 or 120 nM) or vehicle for 6 h. The BMDMs were harvested and stained with 7-aminoactinomycin D (7-AAD), using the APC Annexin V Apoptosis Detection Kit (BioLegend), following the manufacturer’s instructions. Apoptotic cells were detected by flow cytometry using a BD FACSCanto II (BD Biosciences). Data were analyzed with FlowJo Software version 10.7.1 (BD Biosciences).
Mantuano E., Azmoon P., Banki M.A., Sigurdson C.J., Campana W.M, & Gonias S.L. (2021). A soluble PrPC derivative and membrane-anchored PrPC in extracellular vesicles attenuate innate immunity by engaging the NMDA-R/LRP1 receptor complex. Journal of immunology (Baltimore, Md. : 1950), 208(1), 85-96.
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6

Cell Cycle and Apoptosis Analysis

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The control cells and cells intermittently treated with PTH were collected for cell cycle detection by FACScan flow cytometry (BD Biosciences, San Jose, CA) according to the manufacturer's manual. Flow cytometry was also used to study the apoptotic characteristics of cells intermittently treated with PTH according to the instructions of the APC-Annexin V Apoptosis Detection Kit (BioLegend). Three independent experiments were conducted.
Pang X., Zhuang Y., Li Z., Jing S., Cai Q., Zhang F., Xue C, & Yu J. (2020). Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways. Stem Cells International, 2020, 5128128.
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7

Apoptosis Detection via Flow Cytometry

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Flow cytometry was performed to detect cell apoptosis. The APC Annexin V Apoptosis Detection Kit (Biolegend, San Diego, CA, USA) was used for Annexin V staining. The cells were seeded in a 35 mm culture dish at a density of 1 × 10 5 cells/dish. After overnight incubation, the cells were treated with DMSO or LLL12B for 8–12 h. The cells were harvested and stained with Annexin V-APC and propidium iodide (PI) according to the manufacturer’s instructions and after staining were counted using a FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA).
Pan L., Chen X., Rassool F.V., Li C, & Lin J. (2022). LLL12B, a Novel Small-Molecule STAT3 Inhibitor, Induces Apoptosis and Suppresses Cell Migration and Tumor Growth in Triple-Negative Breast Cancer Cells. Biomedicines, 10(8), 2003.
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8

Cell Proliferation and Apoptosis Assay

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Cell counting assays at different times were used to assess cell proliferation ability. Transfected cells were seeded in 24-well plates. Following incubation for 0, 24, 72, or 96 h, homogenized single cell suspension and trypan blue were mixed 1:1. The cells were counted directly under a light microscope with the inclusion of three counting replicates per well. An APC Annexin V Apoptosis Detection kit (Biolegend, San Diego, CA, United States) was used to analyze cells following the manufacturer’s instructions. A BD Accuri C6 flow cytometer was used to analyze the samples.
Zheng W., Lai G., Lin Q., Issah M.A., Fu H, & Shen J. (2021). A miR-129-5P/ARID3A Negative Feedback Loop Modulates Diffuse Large B Cell Lymphoma Progression and Immune Evasion Through Regulating the PD-1/PD-L1 Checkpoint. Frontiers in Cell and Developmental Biology, 9, 735855.
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9

Apoptosis Analysis by Flow Cytometry

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Apoptosis was assessed with an APC Annexin V Apoptosis Detection Kit (BioLegend) following the manufacturer’s protocol. Mouse samples from bone marrow and peripheral blood were stained with human CD45 (Invitrogen Cat. No. MHCD4528) and murine CD45 (Biolegend Cat. No. 103113) antibodies, unless the cells had been previously infected with a fluorescence marker (GFP, mcherry or mAmetrine). Flow cytometry was performed on a LSR Fortessa or FACS Celesta flow cytometer. Cells were sorted on a FACSAria II flow cytometer (BD Biosciences). The data were analyzed with FloJo software (TreeStar).
Ellegast J.M., Alexe G., Hamze A., Lin S., Uckelmann H.J., Rauch P.J., Pimkin M., Ross L.S., Dharia N.V., Robichaud A.L., Conway A.S., Khalid D., Perry J.A., Wunderlich M., Benajiba L., Pikman Y., Nabet B., Gray N.S., Orkin S.H, & Stegmaier K. (2022). Unleashing cell-intrinsic inflammation as a strategy to kill AML blasts. Cancer discovery, 12(7), 1760-1781.
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10

Quantifying Apoptosis by Flow Cytometry

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Cell apoptosis was determined by flow cytometry using the APC Annexin V apoptosis detection kit with propidium iodide (PI) staining (Biolegend, 640932). According to the manufacturer's instructions, cells were washed with PBS twice, and then they were incubated with 5 μl of Annexin V-APC and 10 μl of PI for 15 min at RT in the dark. The numbers of Annexin V-APC-positive cells or/and PI-positive cells were measured by flow cytometry (BD Bioscience, FACSCanto II 488N).
Cui Y., Chen W., Du L, & He Z. (2023). OIP5 Interacts with NCK2 to Mediate Human Spermatogonial Stem Cell Self-Renewal and Apoptosis through Cell Cyclins and Cycle Progression and Its Abnormality Is Correlated with Male Infertility. Research, 6, 0162.
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