Milli q advantage a10 water purification system

Fluorimetry studies were performed on a Photon Technology International fluorimeter with lamp power supply model LPS-220B, motor driver model MD-5020, and shutter control model SC-500. ¹H-NMR studies were performed on a Bruker AV-III 400 MHz NMR spectrometer with ICON NMR in automation, while ¹³C NMR studies were run at 100 MHz. CDCl₃ was used as solvent, chemical shifts are reported in ppm, while coupling constants, J, are reported in Hertz. Multiplicities in the spectra are represented by d (doublet), t (triplet), m (multiplet), and b (broad). High-resolution mass spectra (HRMS) were recorded using a Waters Synapt G2; spectra are reported as m/z (relative intensity). 1 μL of saturated LiCl in acetonitrile was added to 50 μL of sample, masses are reported for either [M+] or [M+H⁺] ion. Millipore water was obtained from a Milli-Q Advantage A-10 water purification system (MilliPore, USA). Microscopy images were captured using a Zeiss Axio Imager A2. Nanoparticle Tracking Analysis was performed on a Malvern NanoSight LM10.

1,2-Phenanthrenequinone (1,2-PheQ) and 3,4-phenanthrenequinone (3,4-PheQ) were prepared as previously described (Figure 3) (Magdziak et al., 2002 (link)).) [¹³C₄]1,2-PheQ was similarly prepared from [¹³C₄]1-hydroxyphenanthrene ([¹³C₄]1-OHPhe), Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). 1-Hydroxyphenanthrene (1-OHPhe) and 4-hydroxyphenanthrene (4-OHPhe) were obtained from Toronto Research Chemicals (North York, ON, Canada). 9,10-Phenanthrenequinone (9,10-PheQ), benzaldehyde, formic acid, ammonium acetate and sodium acetate were purchased from Millipore Sigma (St. Louis, MO, USA). 8-iso-PGF_2α and [D₄]8-iso-PGF_2α were procured from Cayman Chemical (Ann Arbor, MI, USA). β-Glucuronidase and arylsulfatase (from Helix pomatia) were obtained from Roche Diagnostics Corp. (Indianapolis, IN, USA). Oasis® PRiME HLB SPE cartridges (200 mg/6 mL/# 186008057) were from Waters (Milford, MA, USA). A Bond Elut C18 96 square-well plate (100 mg/2 mL/# A396011C) was purchased from Agilent (Santa Clara, CA, USA). All solvents used in this study including hexane, isopropanol, acetonitrile, ethyl acetate and methanol were HPLC grade and obtained from Fisher Scientific (Fair Lawn, NJ, USA). Ultrapure H₂O from a Millipore Milli-Q Advantage A10 Water Purification System was used throughout the study.

Acrylamide (AAm) and N,N′-bis(acryloyl)cystamine (BAC) were purchased from Alfa Aesar. Tetramethylethylenediamine (TEMED) and Amplex UltraRed reagent (AUR) were purchased from ThermoFisher Scientific. Peroxidase from horseradish (HRP), 3-(trimethoxysilyl) propyl methacrylate, dextran from Leuconostoc spp (M_r ~ 100000), and anhydrous (≥99.9%) dimethyl sulfoxide were purchased from Sigma-Aldrich. Ammonium persulfate (APS) was purchased from CalabrioChem Inc. Polyethylene(glycol) diacrylate (PEGDA; M_w = 400) was purchased from PolySciences Inc. Ethanol (EtOH) was purchased from Decon Laboratories Inc. Glacial acetic acid was purchased from Macron Fine Chemicals. Water (dd-H₂O) was ionized using Milli-Q Advantage A-10 water purification system (Millipore, U.S.A.). Microscopic glass slides (3” × 1” × 1.2 mm) and micro cover glass (18 mm × 18 mm) were purchased from VWR. Rain-X water repellant manufactured by ITW (Global Bands, TX) was purchased from Home Depot. All UV−vis absorbance spectra were acquired on a Safire2 UV−vis microplate reader (TECAN, Switzerland). The absorbance for the samples were measured at 568 nm in a Nunclon 96-clear Microwell plate. Mechanical characterization tests were performed on a TA Instruments RSA-G2 Solids Analyzer.