Axiozoom microscope

Manufactured by Zeiss

Sourced in Germany, United States

The Axiozoom microscope is a high-performance optical instrument designed for a wide range of applications. It features a zoom function that allows for continuous adjustment of the magnification, enabling users to easily observe and analyze samples at different scales. The Axiozoom microscope is built with advanced optics and precision engineering to deliver clear, high-quality images.

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Lab products found in correlation

Axio observer z1, by Zeiss (6 mentions) Lsm 780, by Zeiss (4 mentions) Axiocam, by Zeiss (3 mentions) Lsm 780 confocal microscope, by Zeiss (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Alexa488 coupled anti gfp, by Thermo Fisher Scientific (2 mentions) Alexa 555 coupled secondary antibodies, by Thermo Fisher Scientific (2 mentions) Sp8 scanning confocal microscope, by Leica (2 mentions) Lsm 710, by Zeiss (2 mentions) Fluorescence conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Rna bee, by AMS Biotechnology (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Image pro, by Zeiss (1 mentions) Uplsapo10x, by Olympus (1 mentions) Fv1000 confocal laser scanning microscope, by Olympus (1 mentions) Axio observer microscope, by Zeiss (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Axiocam 506 mono camera, by Zeiss (1 mentions) Filter set 38 he, by Zeiss (1 mentions) Hxp 200 c metal halide lamp, by Zeiss (1 mentions)

Close spelling variants of this product

AxioZoom (62 citations) AxioZoom.V16 stereozoom microscope (19 citations) AxioZoom.V16 fluorescence microscope (12 citations) AxioZoom V16 Fluorescence Stereo Zoom Microscope (9 citations) AxioZoom fluorescent microscope (5 citations) AxioZoom.V16 zoom microscope (3 citations) AxioZoom upright microscope (2 citations) Axiozoom epifluorescence microscope (2 citations) Axiozoom.v16 epifluorescence microscope (2 citations) Axiozoom confocal microscope (2 citations)

64 protocols using axiozoom microscope

Imaging Drosophila Hemocytes and GFP

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For sagittal sections of whole flies, decapitated adult female flies were fixed overnight in 4% paraformaldehyde, then manually sectioned using a fine razorblade (Personna by Accutec, Cat No. 74-0002). After antibody staining, bisected flies were placed in a drop of Vectashield mounting media in a 35mm, glass-bottom imaging µ-Dish (Ibidi, Cat. No. 81158.) Tissues were dissected in PBS, fixed for 20–30 minutes in 4% paraformaldehyde, and stained using standard protocols. GFP was detected using either Alexa488-coupled anti-GFP (Invitrogen A21311, used at 1:400) or chicken anti-GFP (Aves Lab GFP1020, used at 1:2000.) Hemocytes were stained using the pan-hemocyte H2 antibody (39 (link)) (Gift of Andó lab, used at 1:100.) Primary antibodies were detected with Alexa-488 or Alexa-555 coupled secondary antibodies (Molecular Probes.) Confocal imaging was performed on either a Zeiss LSM 780 or Zeiss Axio Observer Z1 with a LSM980 Scan Head, with the “Tile Scan” feature for whole guts using system defaults. Whole-larva imaging was performed on a Zeiss AxioZoom microscope. Mean pixel intensity was measured using FIJI/ImageJ, based on maximum intensity projections, with GFP+ pixels selected as regions of interest.

Ewen-Campen B., Luan H., Xu J., Singh R., Joshi N., Thakkar T., Berger B., White B.H, & Perrimon N. (2023). split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80. bioRxiv.

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Nematode Morphological Identification

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To verify that all the nematodes belonged to the same species, we performed a detailed morphological investigation on a subset of the population (adults and juveniles). Several nematodes were mounted on slides for detailed morphological observation using the formalin–ethanol/glycerol technique^{54 (link),55}. Photos were captured on a Leica DM IRB microscope and a Zeiss AxioZoom microscope, each equipped with live camera (Image-Pro and Zen software, respectively).

Bellec L., Bonavita M.A., Hourdez S., Jebbar M., Tasiemski A., Durand L., Gayet N, & Zeppilli D. (2019). Chemosynthetic ectosymbionts associated with a shallow-water marine nematode. Scientific Reports, 9, 7019.

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Quantifying Inhibitory Synaptic Puncta in Brain Slices

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Images were derived from FV1000 confocal laser scanning microscope (Olympus) using UPLSAPO 10X NA:0.40, UPLSAPO 40 × 2 NA:0.95, UPLSAPO 60X O NA:1.35 objective lens and Axio zoom microscope (Carl Zeiss). Images were analyzed by ImageJ (NIH) program. Identical acquisition parameters were used for all the brain slices within a single set of experiment. Image analysis was not performed blind to the genotypes of animals. Inhibitory synapse was defined as co-localization between inhibitory presynaptic marker (VGAT) and inhibitory postsynaptic marker (Gephyrin). Puncta analyzer ver2.0 plugin for ImageJ was downloaded from github (https://github.com/physion/puncta-analyzer) and used to quantify the inhibitory synaptic puncta. The protocol using the Puncta analyzer is well described in the previous article^{62 (link)}.

Kim H.Y., Yang Y.R., Hwang H., Lee H.E., Jang H.J., Kim J., Yang E., Kim H., Rhim H., Suh P.G, & Kim J.I. (2019). Deletion of PLCγ1 in GABAergic neurons increases seizure susceptibility in aged mice. Scientific Reports, 9, 17761.

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Microscopic Imaging of Intestines and Brains

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Images of whole dissected intestines and brains were taken on a Zeiss Axio Zoom microscope. Images of immunostained intestines for quantification were taken on a Zeiss Axio Observer microscope. Image files were prepared using Adobe Photoshop and figures were assembled using Adobe Illustrator.

Holsopple J.M., Cook K.R, & Popodi E.M. (2022). Identification of novel split-GAL4 drivers for the characterization of enteroendocrine cells in the Drosophila melanogaster midgut. G3: Genes|Genomes|Genetics, 12(6), jkac102.

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Measuring Blood-Brain Barrier Permeability

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To investigate BBB permeability, 100 μl TMR Biocytin (AnaSpec AS‐60658; reconstituted with sterile PBS; MW = 869 Da) was delivered by tail‐vein injection to mice 20 min prior to cardiac perfusion. Following brain dissection and coronal slicing (300 μm thick by vibratome), tissues were imaged using a Zeiss Axio Zoom microscope with TMR emission filter settings. Fluorescence intensity was measured from slices spanning the entire rostral‐caudal area of the brain. Mean intensity was compared across treatments.
As an additional permeability measure, slices were stained for anti‐mouse IgG, which should not be present in the brain parenchyma. For staining, thick slices were cleared (20% DMSO and 2% Triton X‐100 in PBS) for 1 week, blocked in 4% normal goat serum overnight at room temperature, and incubated with Alexa Fluor 488 goat anti‐mouse IgG for 6 days at 4°C. After four, 1‐h washes in PBS at room temperature, the tissue was imaged by two‐photon microscopy using a 20x‐W/1.0 NA objective and 5× zoom factor. The mean fluorescence intensity was averaged across three separate images per slice, and compared between mice.

Bauer K.C., York E.M., Cirstea M.S., Radisavljevic N., Petersen C., Huus K.E., Brown E.M., Bozorgmehr T., Berdún R., Bernier L., Lee A.H., Woodward S.E., Krekhno Z., Han J., Hancock R.E., Ayala V., MacVicar B.A, & Finlay B.B. (2022). Gut microbes shape microglia and cognitive function during malnutrition. Glia, 70(5), 820-841.

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Dissection and Imaging of Drosophila Hemocytes

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For sagittal sections of whole flies, decapitated adult female flies were fixed overnight in 4% paraformaldehyde, then manually sectioned using a fine razorblade (Personna by AccuTec, Cat No. 74-0002). After antibody staining, bisected flies were placed in a drop of VECTASHIELD mounting media in a 35-mm, glass-bottom imaging µ-Dish (Ibidi, Cat. No. 81158). Tissues were dissected in PBS, fixed for 20 to 30 min in 4% paraformaldehyde, and stained using standard protocols. GFP was detected using either Alexa488-coupled anti-GFP (Invitrogen A21311, used at 1:400) or chicken anti-GFP (Aves Lab GFP1020, used at 1:2,000). Hemocytes were stained using the pan-hemocyte H2 antibody (39 (link)) (Gift of Andó lab, used at 1:100). Primary antibodies were detected with Alexa-488 or Alexa-555 coupled secondary antibodies (Molecular Probes). Confocal imaging was performed on either a Zeiss LSM 780 or Zeiss Axio Observer Z1 with a LSM980 Scan Head, with the “Tile Scan” feature for whole guts using system defaults. Whole-larva imaging was performed on a Zeiss AxioZoom microscope. Mean pixel intensity was measured using FIJI/ImageJ, based on maximum intensity projections, with GFP+ pixels selected as regions of interest.

Ewen-Campen B., Luan H., Xu J., Singh R., Joshi N., Thakkar T., Berger B., White B.H, & Perrimon N. (2023). split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80. Proceedings of the National Academy of Sciences of the United States of America, 120(24), e2304730120.

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Detailed Microscopy Imaging Protocol

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Except where noted below, imaging was performed on an upright Zeiss AxioZoom microscope equipped with a HXP 200C metal halide lamp, PlanNeoFluor Z 1x objective (0.25 NA, FWD 56 mm), and Axiocam 506 mono camera. For fluorescence imaging, filters used were Zeiss Filter Set 38 HE (Ex: 470/40, Em: 525/50), 43 HE (Ex: 550/25, Em: 605/70); 64 HE (Ex: 587/25, Em: 647/70); and 49 HE (Ex: 365, Em: 445/50). Brightfield images were collected using transmitted light. Zen 2/3 Blue software was used for image collection, and images were analyzed in ImageJ v1.52k.
For imaging cells after overnight culture, we used a Zeiss AxioObserver 7 inverted microscope equipped with a Colibri.7 LED light source, EC Plan-Neofluar 5x objective (N.A.=0.16, WD=18.5 mm), and ORCA-Flash4.0 LT+ sCMOS camera (Hamamatsu). For fluorescence imaging, the filter used was a Zeiss 112 HE LED penta-band. Zen 3 Blue software was used for image collection.

Ortiz-Cárdenas J.E., Zatorski J.M., Arneja A., Montalbine A.N., Munson J.M., Luckey C.J, & Pompano R.R. (2022). Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device. Organs-on-a-chip, 4, 100018.

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Patterning of hydrogel biomaterials on microfluidic chips

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Chips were assembled as described above, with the bottom layer comprised of either a coverslip (0.13 – 0.16 mm thickness) or a 1-mm thick Corning® Glass Slides, 75 x 50 mm (Ted Pella, Inc.). In the case of GelSH, a precursor solution composed of 5% GelSH, 0.313- or 1.25-mM PEG-NB linker, 3.4 mM LAP, and 5 μM NHS-Rhodamine was patterned on-chip as described above, using a 45 second exposure at 50 mW/cm². In the case of GelMA, a precursor solution composed of 10% GelMA, 3.4 mM LAP, and 5 μM NHS-Rhodamine was patterned on-chip as described above, using exposure at 50 mW/cm². The exposure times were 119 and 30 seconds for 70% and 32% DOF GelMA, respectively. For GelMA samples, the chips were placed in the incubator immediately for 1 min after light exposure to reduce the viscosity of the material for rinsing. Un-crosslinked material was rinsed out with 1x PBS for 5 min at 5 μL/min, after which the inlet and outlet were closed using TT-30 (Weico Wire) tubing filled with PDMS. The chips were placed in an incubator (37 °C, 5% CO₂) for 30 minutes in the absence of flow, then chips rinsed once more with 1x PBS for 5 minutes at 5 μL/min. Features were imaged by brightfield and fluorescence microscopy on a Zeiss AxioZoom microscope (HE 43 filter set). The diameter of each feature was quantified from the fluorescence images by using line tools in ImageJ v1.52k.

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Whole-Mount β-Galactosidase Staining

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Fixed embryos (as above) were permeabilised in 1% Triton X-100 and 0.4% NP40 in PBS for 4 h and then incubated overnight for β-galactosidase activity at 37°C as described (Tajbakhsh et al., 1994 (link)). Embryos were imaged using a Zeiss Axio Zoom microscope and Zeiss Zen software was used for image analysis.

Aldeiri B., Roostalu U., Albertini A., Wong J., Morabito A, & Cossu G. (2017). Transgelin-expressing myofibroblasts orchestrate ventral midline closure through TGFβ signalling. Development (Cambridge, England), 144(18), 3336-3348.

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Naïve CD4+ T Cell Culture and Viability Assessment

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Human naïve CD4+ T cells used in this work were sourced and prepared as described previously [14 (link)]. Briefly, T cells were purified from TRIMA collars from healthy donors (collars purchased from INOVA Laboratories, Sterling, VA, USA) and were isolated using human CD4+ T cell RosetteSep™ kit (STEMCELL Technologies, Vancouver, BC, Canada) and Ficoll-Paque (Cytiva Inc., Marlborough, MA, USA) density centrifugation, and enriched by immuno-magnetic negative selection with the EasySep™ Naïve CD4+ T cell isolation kit (STEMCELL Technologies).
The conditioned media (effluent) from Section 2.4 was used to culture primary human naïve CD4+ T cells at 1 × 10⁶ cells/mL in a tissue culture treated 96-well plate for 24 h. Cell viability was assessed by staining cells with 10 μM Calcein-AM and 1 μM DAPI in PBS for 30 min at 37 °C, rinsing with 5× volume PBS, and imaging with a Zeiss AxioZoom microscope. ImageJ was used to identify and count cells, using the Count Particles function with a circularity of 0.5–1 and a size 12.5–500 µm². Percent viability was defined as Calcein positive/(Calcein positive + DAPI positive).

Musgrove H.B., Saleheen A., Zatorski J.M., Arneja A., Luckey C.J, & Pompano R.R. (2023). A Scalable, Modular Degasser for Passive In-Line Removal of Bubbles from Biomicrofluidic Devices. Micromachines, 14(2), 435.

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