Anti pin1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Pin1 is a laboratory product developed by Santa Cruz Biotechnology. It is a research tool used to detect and study the Pin1 protein, which is involved in various cellular processes. The product functions as an antibody that specifically binds to the Pin1 protein, allowing researchers to identify and analyze its presence and distribution in biological samples.

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Lab products found in correlation

Anti α tubulin, by Santa Cruz Biotechnology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti notch3 m20, by Santa Cruz Biotechnology (1 mentions) Anti flag hrp, by Merck Group (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti mpm2, by Merck Group (1 mentions) Anti notch1val1744, by Cell Signaling Technology (1 mentions) Anti notch3, by Cell Signaling Technology (1 mentions) Anti lck, by Santa Cruz Biotechnology (1 mentions) Anti lamin b m 20, by Santa Cruz Biotechnology (1 mentions) Anti hes1, by Santa Cruz Biotechnology (1 mentions) Ecl chemiluminescence reagent, by GE Healthcare (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Anti actin, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti tlr7, by Thermo Fisher Scientific (1 mentions) Imject alum, by Thermo Fisher Scientific (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions) Anti xbp1, by Abcam (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions)

Close spelling variants of this product

Anti-Pin1 antibody (2 citations)

5 protocols using anti pin1

Protein Interaction Analysis Techniques

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λ-Phosphatase treatment,^{19 (link)} GST pull down,^{19 (link)} far western,^{19 (link)} protein extracts preparation,^{12 (link)} immunoprecipitation,^{12 (link)} immunoblotting assays^{12 (link)} and biotinylation assay^{12 (link), 62 (link)} were performed as previously described. Immunoblot analysis was performed using the following antibodies: anti-Flag (Sigma, Cat#F3165), anti-Flag-HRP (Sigma; Cat#A8592), anti-MPM2 (05-368, Millipore), anti-Notch1_Val1744 (Cell Signaling, Danvers, MA, USA, Cat#2421), anti-Notch3 (Cell Signaling; Cat#2889); anti-Notch3 M20 (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-7424), anti-Pin1 (Santa Cruz Biotechnology; Cat#sc-46660), anti-β-actin (Santa Cruz Biotechnology; Cat#sc-47778), anti-Lck (Santa Cruz Biotechnology; Cat#sc-433), anti-α-tubulin (Santa Cruz Biotechnology; Cat#sc-803), anti-LaminB M20 (Santa Cruz Biotechnology; Cat#sc-6217) and anti-Hes1 (Santa Cruz Biotechnology; Cat#sc-25392). The antibody against the activated Notch3-IC protein (N3_IC-act) was kindly provided by Genentech (South San Francisco, CA, USA). The anti-N3_EC (5E1) antibody was kindly provided by Professor A Joutel.^{62 (link)} The anti-pTα antibody was kindly provided by H von Bohemer.^{63 (link)}

Franciosa G., Diluvio G., Gaudio F.D., Giuli M.V., Palermo R., Grazioli P., Campese A.F., Talora C., Bellavia D., D'Amati G., Besharat Z.M., Nicoletti C., Siebel C.W., Choy L., Rustighi A., Sal G.D., Screpanti I, & Checquolo S. (2016). Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression. Oncogene, 35(36), 4741-4751.

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Antibodies for RNA-Binding Proteins

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Rabbit polyclonal anti-Pin1 and mouse monoclonal antihemagglutinin (anti-HA) antibodies were obtained from Santa Cruz. An in-house rabbit polyclonal anti-SLBP antibody made toward the C terminal 13 amino acids of human SLBP was used for the Western blots. Antibodies toward the following human proteins were a gift from Dr. Ann-Bin Shyu (University of Texas Health Science Center): goat polyclonal antibody towards the C-terminus of human FBP1, mouse monoclonal antibody towards KSRP, mouse monoclonal CUGBP1, goat polyclonal against a 20 amino acid C-terminal peptide of TIA1, goat polyclonal antibody towards 18 C-terminal amino acids of TIAR, rabbit polyclonal antibody against amino acids 166–285 of TTP, mouse monoclonal antibody towards HuR, and a rabbit polyclonal antibody towards BRF1.

Krishnan N., Titus M.A, & Thapar R. (2014). The Prolyl Isomerase Pin1 Regulates mRNA Levels of Genes with Short Half-Lives by Targeting Specific RNA Binding Proteins. PLoS ONE, 9(1), e85427.

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Western Blot Analysis of Cellular Proteins

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The anti-ß-actin (1:2000) antibody was purchased from Sigma (St. Louis, MO), the anti-Pin1 (1:1000) antibody from Santa Cruz Biotechnology (Santa Cruz, CA), and the anti-rabbit/mouse horseradish peroxidase (HRP) secondary antibody (1:2000) from GE Healthcare (Little Chalfont, UK). The cells were solubilized with Laemmli buffer (0.2 M Tris·HCl, 4% SDS, 10% glycerol, 5% 2-mercaptoethanol and 0.1% bromophenol blue). Equal amounts of protein from whole cell lysates were resolved by SDS-PAGE. The proteins were then transferred to polyvinylidene difluoride membranes (Millipore, MA). The membranes were blocked with 3% nonfat dry milk or 5% bovine serum albumin in Tris-buffered saline with 0.1% Tween 20 and incubated with specific antibodies, followed by incubation with HRP-conjugated secondary antibodies. The antigen-antibody interactions were visualized by incubation with ECL chemiluminescence reagent (GE Healthcare).

Kanaoka R., Kushiyama A., Seno Y., Nakatsu Y., Matsunaga Y., Fukushima T., Tsuchiya Y., Sakoda H., Fujishiro M., Yamamotoya T., Kamata H., Matsubara A, & Asano T. (2015). Pin1 Inhibitor Juglone Exerts Anti-Oncogenic Effects on LNCaP and DU145 Cells despite the Patterns of Gene Regulation by Pin1 Differing between These Cell Lines. PLoS ONE, 10(6), e0127467.

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Immunological Reagents for Signaling Pathways

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Anti-TLR7 (against N-terminal for WB), anti-ATF4 and anti-Xbp1 were from Abcam. Anti-TLR7 (against C-terminal for WB) was purchased from Cell Signaling. Anti-TLR7 (against N-terminal) from Invitrogen, Anti-TLR7 (against C-terminal) and Anti-TLR7 (against C-terminal for IF) from Noves Biologicals. Anti-Pin1 and anti-ATF6 from Santa Cruz Biotechnology. SYBR Green PCR Master Mix was from Bio-Rad. R848, OVA and anti-β-actin were from Sigma. PCR primers were purchased from IDT, Inc. Protease Inhibitor Mixture was from Calbiochem. The DyLight 800/680 secondary antibodies and IMJECT Alum were purchased from Thermo Scientific. TaqMan Universial PCR Master Mix (for Rhinovirus) and TaqMan Gene Expression Assay (for SeV) were from Applied Biosystem. Sendai virus was from ATCC. Mouse TLR1–9 Agonist kit was from InvivoGen. Murine IL-5, mSCF and mFLT3L were from PeproTech. EDN (RNase2) ELISA kit, MBP (Prg2) ELISA kit and ECP ELISA kit were from MyBioSource. Anti-PRG2, anti-ECP and anti-EDN were from Biorbyt. LEGENDplx Mouse Proinflammatory Chemokine Panel was from Biolegend.

Shen Z.J., Hu J., Kashi V., Bochkov Y.A., Gern J.E, & Malter J.S. (2018). TLR-7 stress signaling in differentiating and mature eosinophils is mediated by the prolyl isomerase Pin1. Journal of immunology (Baltimore, Md. : 1950), 201(12), 3503-3513.

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Immunofluorescence Staining of Pin1 and Phospho-T19

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Three to five days post-transfection cells were fixed in 4% Paraformaldehyde at room temperature for 20 min. Cells were then rinsed three times with 1× PBS, then 5 min in 50 mM NH4Cl, and three more quick rinses in 1× PBS. Cells were permeabilized in 0.1% Tx100 PBS for 5 min followed by three quick PBS rinses and incubated in 2 mL of 1%BSA in PBS for 45 min followed by incubation in 100 μl of anti-Pin1 (Santa Cruz Biotechnology, Dallas, TX, USA) and the anti-phospho T19 (1:500) for 1 h. Cells were rinsed 3× in PBS and the Alexa fluor 647 anti-mouse (1:500), and Alexa fluor 488 anti-rabbit (1:500) for 1 h in 1% BSA in PBS. Cells were rinsed 5× in PBS, postfix in 4% PFA and mounted in slow fade mounting media (Life Technologies, Carlsbad, CA, USA).

, & Delgado J.Y. (2020). An Alternative Pin1 Binding and Isomerization Site in the N-Terminus Domain of PSD-95. Frontiers in Molecular Neuroscience, 13, 31.

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