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W w phosphoric acid

Manufactured by Merck Group
Sourced in Italy

85% w/w phosphoric acid is a laboratory reagent used for various analytical and experimental purposes. It is a clear, colorless, and odorless liquid with a high concentration of phosphoric acid. The product is commonly used as a pH adjuster, a buffer solution, or a cleaning agent in laboratory settings.

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4 protocols using w w phosphoric acid

1

Standardization of Soybean Phospholipid Mixture

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Lipoid S75 (S75), a mixture of soybean phospholipids (70% phosphatidylcholine, 9% phosphatidylethanolamine, and 3% lysophosphatidylcholine), triglycerides, and fatty acids, was purchased from Lipoid GmbH (Ludwigshafen, Germany). Ethanol, UPLC-gradient grade acetonitrile, methanol, 85% w/w phosphoric acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and all the other reagents, if not otherwise specified, were purchased from Sigma-Aldrich/Merck (Milan, Italy). Standard quercetin-3-O-rhamnoside was purchased from Extrasynthese (Genay Cedex, France). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System (Millipore, Milan, Italy).
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2

Phospholipid-Based Antioxidant Extraction

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Phospholipon90G (P90G; phosphatidylcholine content ≥ 94%) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Propylene glycol (PG) were purchased from Galeno Srl (Comeana, Prato, Italy). LC-MS grade acetonitrile, formic acid, methanol, 85% w/w phosphoric acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tris(pyridin-2-yl)-1,3,5-triazine (TPTZ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), dimethyl sulphoxide (DMSO), and all the other reagents, if not otherwise specified, were purchased from Sigma-Aldrich/Merck (Milan, Italy). Standards of 5-O-caffeoylquinic (chlorogenic acid), 1,3-dicaffeoylquinic acid (cynarin), 3,5-dicaffeoylquinic acid (isochlorogenic acid A), 3,4-dicaffeoylquinic acid (isochlorogenic acid B), 4,5-dicaffeoylquinic acid (isochlorogenic acid C), ferulic acid, apigenin, hispidulin, and luteolin were purchased from Extrasynthese (Genay, France) and TransMIT (Giessen, Germany). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).
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3

Polyphenol Extraction and Analysis

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Ethanol absolute was from VWR (Milan, Italy); methanol and 85% w/w phosphoric acid were from Sigma-Aldrich (Steinheim, Germany); LC–MS-grade acetonitrile, formic acid, and water were from Merck (Darmstadt, Germany). Standards of gallic acid, methyl gallate, ethyl gallate, myricetin 3-O-glucoside, quercetin, quercetin-3-O-rhamnoside, and kaempferol-3-O-glucoside were from Extrasynthese (Genay, France) and TransMIT (Giessen, Germany). Phospholipon90G (>94% phosphatidylcholine; P90G) was from Lipoid GmbH (Ludwigshafen, Germany). All the reagents for cell culture were provided by Lonza (Verviers, Belgium).
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4

Analytical Quantification of Phenolic Compounds

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Ethanol absolute was purchased from VWR (Milano, Italy); methanol and 85% w/w phosphoric acid were purchased from Sigma-Aldrich (Steinheim, Germany). LC-MS grade acetonitrile, formic acid, and water were purchased from Merck (Darmstadt, Germany). Standards of cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, peonidin-3-O-glucoside, peonidin-3-O-rutinoside, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, p-coumaric acid, ferulic acid, vanillic acid, 3-O-caffeoylquinic acid (neochlorogenic acid), and 5-O-caffeoylquinic acid (chlorogenic acid) were purchased from Extrasynthese (Genay, France) and TransMIT (Giessen, Germany).
Soy lecithin (a mixture of polar phospholipids and glycolipids) and propylene glycol (PG) were purchased from Galeno Srl (Comeana, Prato, Italy).
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