UV spectra of the isolated compounds were detected on a Hitachi-300 spectrophotometer (Kyoto, Japan). NMR spectra of isolated compounds were measured in CDCl3 using Bruker DRX-600 MHz Ultrashield spectrometers (Bruker BioSpin, Billerica, MA, USA). Isolation and purification of compounds were performed on column chromatography using Diaion HP-20 and silica gel 60 (70–230 mesh, Merck, Darmstadt, Germany). Monitoring of eluted compounds was performed on pre-coated TLC plates with silica gel 60 F254 (Merck, Darmstadt, Germany).
300 spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The -300 spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light through a sample. It is capable of analyzing the light absorption or transmission properties of various materials within a specific wavelength range.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 300 spectrophotometer
1
Isolation and Characterization of Compounds
2
Analytical Techniques for Compound Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ultraviolet (UV) spectra were measured on a Hitachi 300 Spectrophotometer. Nuclear magnetic resonance (NMR) spectra were obtained in CD3Cl and CD3OD on Bruker Avance DRX 600 spectrometers at 600 MHz for hydrogen NMR (1H NMR) and 150 MHz for carbon NMR (13C NMR). Mass spectral data were performed on ion trap liquid chromatography/mass spectrum agilent. For column chromatography, silica gel (Merck, 70–230 mesh ASTM) and sephadex LH-20 (pharmacia) were used. Precoated silica gel 60 F-254 plates (Merck) were used for thin-layer chromatography (TLC). Reversed phase-18 (40–63 μm), 6 ml standard tubes (extraction tubes), Damstadt, Germany, were used for purification of the polar compounds. Spots were visualized by exposure to NH3 vapour, UV radiation and P-anisaldehyde/sulfuric acid.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!