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E el m0037c

Manufactured by Elabscience
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Sourced in China

E-EL-M0037c is a laboratory equipment produced by Elabscience. It is a device designed for the purpose of performing specific laboratory tasks, though its core function is not explicitly stated.

Automatically generated - may contain errors

Lab products found in correlation

E el m0044c, by Elabscience (13 mentions) E el m0049c, by Elabscience (6 mentions) E el m3063, by Elabscience (5 mentions) E el m0046c, by Elabscience (4 mentions) E el m0730c, by Elabscience (3 mentions) E el m0201c, by Elabscience (2 mentions) E el h0149c, by Elabscience (2 mentions) E el m2453c, by Elabscience (2 mentions) Multiskan go, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit 7074, by Cell Signaling Technology (1 mentions) Anti β actin 60004 1 ig, by Proteintech (1 mentions) Anti nf κb p65 8242, by Cell Signaling Technology (1 mentions) Goat anti mouse 7076, by Cell Signaling Technology (1 mentions) E el r2856c, by Elabscience (1 mentions) E el r0012c, by Elabscience (1 mentions) A003 1 2, by Nanjing Jiancheng (1 mentions) A001 3 2, by Nanjing Jiancheng (1 mentions) A005 1 2, by Nanjing Jiancheng (1 mentions) E el h0109c, by Elabscience (1 mentions) E el h0102c, by Elabscience (1 mentions)

24 protocols using e el m0037c

1

Quantifying Serum IL-1β and IL-18 in MI Mice

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After various treatments, the concentration of IL-1β (E-EL-M0037c, Elabscience, Wuhan, China) and IL-18 (E-EL-M0730c, Elabscience, Ltd, Wuhan, China) in the serum of MI mice was measured by ELISA kit according to the protocols25 (link). Absorbance at 450 nm was measured in each well by visualization of color intensity development.
Li Z., Xu H., Liu X., Hong Y., Lou H., Liu H., Bai X., Wang L., Li X., Monayo S.M., Mokembo J.N., Jha N.K., Yang B, & Zhang Y. (2020). GDF11 inhibits cardiomyocyte pyroptosis and exerts cardioprotection in acute myocardial infarction mice by upregulation of transcription factor HOXA3. Cell Death & Disease, 11(10), 917.
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2

ELISA Analysis of IL-1β and Casp-1

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The IL-1β and casp-1 concentration in the supernatants of RAW 264.7 and THP-1 were assayed by human IL-1β (Elabscience Biotechnology Co., Ltd., E-EL-H0149c, Wuhan, China) and casp-1 (Elabscience Biotechnology Co., Ltd., E-EL-H0016c, Wuhan, China) Elisa kit or mice IL-1β (Elabscience Biotechnology Co., Ltd., E-EL-M0037c, Wuhan, China) and casp-1 (Elabscience Biotechnology Co., Ltd., E-EL-M0201c, Wuhan, China) Elisa kit according to the manufacturer’s instructions.
Cao M, & Cai L. (2022). Nanoparticle Emulsions Enhance the Inhibition of NLRP3. International Journal of Molecular Sciences, 23(17), 10168.
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3

Cornuside-Mediated Neuroprotection: Mechanistic Insights

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The supplier of cornuside (purity ≥ 98%), i.e., C24H30O14 (molecular mass: 542.9), was the Clinical Medicine Research Institute of China-Japan Friendship Hospital. The ELISA (enzyme-linked immunosorbent assay) kits for Aβ1–40 (E-EL-M3009), Aβ1–42 (E-EL-M3010), IL-1β (E-EL-M0037c), IL-6 (E-EL-M2453c), and TNF-α (E-EL-M3063) in mice, as well as for IL-1β (E-EL-R0012c), IL-6 (E-EL-R0896c), and TNF-α (E-EL-R2856c) in rats were products of Elabscience Biotechnology (Wuhan, China). Primary antibodies including anti-Tau (phospho Ser396) (ab32057), anti-GFAP (60190-1-Ig), anti-C3 (21337-1-AP), anti-S100A10 (11250-1-AP), anti-iNOS (18985-1-AP), anti-PSD95 (20665-1-AP), anti-Nrf2 (16396-1-AP), anti-Histone-H3 (17168-1-AP), anti-GAPDH (60004-1-Ig), and anti-β-actin (60004-1-Ig) antibodies were provided by Proteintech (Wuhan, China). Anti-Iba1 (019-19741) antibody was obtained from FUJIFILM Wako Pure Chemical Corporation. Anti-SYP (PB0462) was a product of Boster Biological Technology (Wuhan, China). The provider of anti-p-NF-κB p65 (#3033), anti-p-AKT (Ser473) (#4060), anti-AKT (#4691), anti-IκB-α(#4814), and anti-NF-κB p65 (#8242) antibodies was Cell Signaling Technology (Danvers, MA, USA), which was also the provider of both the goat anti-rabbit (#7074) and goat anti-mouse (#7076) secondary antibodies.
Shi J.Z., Zheng X.M., Zhou Y.F., Yun L.Y., Luo D.M., Hao J.J., Liu P.F., Zhang W.K., Xu J.K., Yan Y., Xie X.M., He Y.Y, & Pang X.B. (2022). Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes. Nutrients, 14(15), 3179.
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4

Oxidative Stress and Neuroinflammation Markers

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Plasma was collected, and the levels of total superoxide dismutase (SOD, A001-3-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), malondialdehyde (MDA, A003-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and glutathione peroxidase (GSH-Px, A005-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) were measured using the corresponding kit according to the manufacturer’s instructions. The levels of Aβ1–40 (E-EL-M300, Elabscience Biotechnology Co., Ltd., Wuhan, China), Aβ1–42 (E-EL-M3010, Elabscience Biotechnology Co., Ltd., Wuhan, China), p-Tau (E-EL-M1289c, Elabscience Biotechnology Co., Ltd., Wuhan, China), IL-1β (E-EL-M0037c, Elabscience Biotechnology Co., Ltd., Wuhan, China), and IL-18 (E-EL-M0730c, Elabscience Biotechnology Co., Ltd., Wuhan, China) in the supernatant from the brain homogenate were assessed using the corresponding enzyme-linked immunosorbent assay (ELISA) kits following the manufacturer’s protocols.
Guo P., Zeng M., Wang S., Cao B., Liu M., Zhang Y., Jia J., Zhang Q., Zhang B., Wang R., Zheng X, & Feng W. (2022). Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25–35-Induced Mice by Inhibiting the NLRP3 Inflammasome. Molecules, 27(8), 2488.
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5

ELISA Assay of Inflammatory Cytokines

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ELISA analysis was performed as previously described (Yan et al., 2021 (link)). The inflammatory cytokines TNF-α, IL-6 and IL-1β in brain tissue or cell culture medium were detected with ELISA kits (Elabscience Biotechnology, E-EL-M0037c for mouse IL-1β, E-EL-H0149c for human IL-1β, E-EL-M0049c for mouse TNF-α, E-EL-H0109c for human TNF-α, E-EL-M0044c for mouse IL-6, E-EL-H0102c for human IL-6). All procedures were performed according to the manufacturer’s instructions.
Zeb S., Ye H., Liu Y., Du H.P., Guo Y., Zhu Y.M., Ni Y., Zhang H.L, & Xu Y. (2023). Necroptotic kinases are involved in the reduction of depression-induced astrocytes and fluoxetine’s inhibitory effects on necroptotic kinases. Frontiers in Pharmacology, 13, 1060954.
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6

Serum Cytokine Profiling in Mice

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Blood was collected from mice under anaesthesia via the ophthalmic vein into EDTA tubes and then centrifuged at 1,500 rpm for 15 min at 4°C. Serum samples were then divided evenly into the Eppendorf (EP) tubes and preserved at −80°C for subsequent experiments. TNF-α (Elabscience, China, #E-EL-M3063), IL-1β (Elabscience, China, #E-EL-M0037c), and IL-6 (Elabscience, China, #E-EL-M0044c) in serum were assayed with ELISA kits.
Jiang Y., Wei Q., Liu W., Chen Q., Chen X., Yuan Z., Luo N., Chen X, & Wang C. (2022). Exploring the Muscle Metabolomics in the Mouse Model of Sepsis-Induced Acquired Weakness. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 6908488.
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7

Quantifying Inflammatory Cytokines in Mice

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At the end of the experiment, blood was obtained from the mice and their serum was collected following centrifugation at 3000 rpm for 30 min. The ELISA kits for measuring IL‐1β (E‐EL‐M0037c), IL‐6 (E‐EL‐M0044c), TNF‐α (E‐EL‐M3063), IL‐10 (E‐EL‐M0046c) and IL‐4 (E‐EL‐M0043c) were purchased from Elabscience (Wuhan, China). The levels of IL‐1β, IL‐6, TNF‐α, IL‐10 and IL‐4 in the mouse serum were quantified using the respective ELISA kits according to the manufacturer's instructions. The absorbance was determined on a microplate reader at OD450 nm.
Jiang H., Wang Y., Tang Z., Peng X., Li C., Dang Y, & Ma R. (2024). Calycosin alleviates titanium particle‐induced osteolysis by modulating macrophage polarization and subsequent osteogenic differentiation. Journal of Cellular and Molecular Medicine, 28(7), e18157.
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8

Cytokine Profiling in Mouse Serum and Cell Supernatants

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Samples of mouse blood serum and the supernatants of HPAEpiCs were collected for analysis of cytokines, including Interleukin 1 Beta (IL-1β), Interleukin 6 (IL-6), Interleukin 17 (IL-17), and monocyte chemotactic protein 1 (MCP-1) by using Mouse IL-1β (E-EL-M0037c, Elabscience, China), IL-6 (E-EL-M0044c, Elabscience), IL-17 (3020092, BioAim Scientific Inc., Toronto, Canada), and MCP-1 (E-EL-M3001, Elabscience) ELISA kits according to the manufacturer's instructions.
Liang Y., Xu Y., Lu B., Huang Y., Xu S., Xie J., Liu M., Che D., Ma L., Tao J., Hong J., Zhang J., Situ X., Ou X., Chen L., Li Y., Zhang L, & Wu Z. (2022). Inositol Alleviates Pulmonary Fibrosis by Promoting Autophagy via Inhibiting the HIF-1α-SLUG Axis in Acute Respiratory Distress Syndrome. Oxidative Medicine and Cellular Longevity, 2022, 1030238.
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9

Cytokine Levels in MLE-12 Cells and BALF

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The levels of proinflammatory cytokines (interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α) in MLE-12 cells and mouse BALF were examined using corresponding commercial ELISA kits (E-EL-M0037c, E-EL-M0044c, E-EL-M3063; Elabscience, Wuhan, China). Absorbance was measured at 450 nm within 25 min of the reaction using a microplate reader (DNM-9606, PERLONG, Beijing, China).
Li Y., Xu H.L., Kang X.W., Xu S, & Mou Z.F. (2023). MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1. Heliyon, 10(2), e22772.
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10

Isolation and Characterization of ARR

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ARR (purity >95%) was isolated from Loranthus tanakae Franch. & Sav. in our laboratory, as previously described (21 (link)). The structure of ARR was elucidated by nuclear magnetic resonance. LPS and indomethacin (Indo) were purchased from Sigma-Aldrich; Merck KGaA. DMEM, FBS and penicillin-streptomycin were purchased from Gibco; Thermo Fisher Scientific, Inc. Primary rabbit monoclonal antibodies, including NF-κB p65 (cat. no. ab16502), phosphorylated-(p)-NF-κB-p65 (cat. no. ab76302), Nrf2 (cat. no. ab92946), NQO1 (cat. no. ab80588), HO-1 (cat. no. ab13243), TRAF6 (cat. no. ab137452), β-actin (cat. no. ab8227) and Histone H3 (cat. no. ab1791) were purchased from Abcam and the NLRC3 antibody (cat. no. DF13411) was obtained from Affinity Biosciences. The nitric oxide (NO) colorimetric kit (cat. no. E-BC-K035-M) and the cytokine mouse ELISA kits specific for prostaglandin E2 (PGE2; cat. no. E-EL-0034c), IL-6 (cat. no. E-EL-M0044c) and IL-1β (cat. no. E-EL-M0037c) were purchased from Elabscience.
Zhou J.T., Ren K.D., Hou J., Chen J, & Yang G. (2021). α-rhamnrtin-3-α-rhamnoside exerts anti-inflammatory effects on lipopolysaccharide-stimulated RAW264.7 cells by abrogating NF-κB and activating the Nrf2 signaling pathway. Molecular Medicine Reports, 24(5), 799.
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