Immunoblot analysis was performed as previously described [22 (link), 23 (link)]. The following antibodies were used: anti-MGMT (1:1,000; Cell Signaling Technology, Danvers, MA, USA), anti-RAGE (1:1,000; Cell Signaling Technology, Tokyo, Japan), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:20,000; Trevigen, Gaithersburg, MD, USA), horseradish peroxidase-linked anti-rabbit IgG (1:20,000; GE Healthcare UK Ltd., Amersham Place, Little Chalfont, UK), and horseradish peroxidase-linked whole antibody anti-mouse IgG (1:20,000; GE Healthcare).
Horseradish peroxidase linked whole antibody anti mouse igg
Manufactured by GE Healthcare
Sourced in United States
Horseradish peroxidase-linked whole antibody anti-mouse IgG is a laboratory reagent used in various immunoassay techniques. It consists of a whole antibody specific to mouse immunoglobulin G (IgG), conjugated with the enzyme horseradish peroxidase. This reagent can be used to detect and quantify the presence of mouse IgG in samples.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase linked anti rabbit igg, by GE Healthcare (6 mentions)
D5b3 monoclonal antibody 9045s, by Cell Signaling Technology (3 mentions)
A19178 200ul, by Merck Group (2 mentions)
Mouse anti dpyd a 5 monoclonal antibody sc 376712, by Santa Cruz Biotechnology (2 mentions)
Anti rage, by Cell Signaling Technology (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Bio-Techne (1 mentions)
Anti mgmt, by Cell Signaling Technology (1 mentions)
Rabbit anti gapdh, by Bio-Techne (1 mentions)
Sc 6954, by Santa Cruz Biotechnology (1 mentions)
Alx 804 220, by Enzo Life Sciences (1 mentions)
6 protocols using horseradish peroxidase linked whole antibody anti mouse igg
1
Immunoblot Analysis of MGMT and RAGE
2
Western Blotting Antibodies Validation
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Western blotting was performed as described previously.24 , 25 The antibodies used were rabbit anti‐thymidylate synthase (D5B3) monoclonal antibody (9045 S; 1:1000, Cell Signaling Technologies), rabbit anti‐LC3 polyclonal antibody (PM036, 1:1000; Medical & Biological Laboratories), mouse anti‐SQSTM1(D‐3) monoclonal antibody (sc‐28359, 1:200; Santa Cruz Biotechnology), mouse anti‐beta‐actin monoclonal antibody (A19178‐200UL, 1:20,000; Sigma‐Aldrich), horseradish peroxidase‐linked anti‐rabbit IgG (1:20,000; GE Healthcare), and horseradish peroxidase‐linked whole antibody anti‐mouse IgG (1:20,000; GE Healthcare). [Correction added on [7th December 2022], after first online publication: sc‐28,358 has been corrected to sc‐28359.]
3
Western Blotting for Protein Expression
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Western blotting analysis was performed
as previously described.16 (link),35 (link) The antibodies used
were rabbit anti-thymidylate synthase (D5B3) monoclonal antibody (9045S,
1:1000, Cell Signaling Technologies, Massachusetts), mouse anti-DPYD
(A-5) monoclonal antibody (sc-376712, 1:1000, Santa Cruz Biotechnology,
Texas), mouse anti-β-actin monoclonal antibody (A19178-200UL,
1:20 000, Sigma-Aldrich), horseradish peroxidase-linked antirabbit
IgG (1:20 000, GE Healthcare, Connecticut), and horseradish
peroxidase-linked whole-antibody antimouse IgG (1:20 000, GE
Healthcare).
as previously described.16 (link),35 (link) The antibodies used
were rabbit anti-thymidylate synthase (D5B3) monoclonal antibody (9045S,
1:1000, Cell Signaling Technologies, Massachusetts), mouse anti-DPYD
(A-5) monoclonal antibody (sc-376712, 1:1000, Santa Cruz Biotechnology,
Texas), mouse anti-β-actin monoclonal antibody (A19178-200UL,
1:20 000, Sigma-Aldrich), horseradish peroxidase-linked antirabbit
IgG (1:20 000, GE Healthcare, Connecticut), and horseradish
peroxidase-linked whole-antibody antimouse IgG (1:20 000, GE
Healthcare).
4
Western Blot Analysis of Cellular Proteins
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Western blot analysis was performed as described previously [30 (link), 31 (link)]. The following antibodies were used: rat anti-NAMPT clone 14A.5 (1:1,000, Millipore), rabbit anti-GAPDH (1:20,000, Trevigen), mouse anti-NAPRT(B-8) (1:1,000, Santa Cruz Biotechnology), rabbit anti-POTEE antibody (1:200, Abcam), mouse monoclonal anti-beta actin clone AC-15 (1:20,000, Sigma-aldrich), horseradish peroxidase-linked anti-rat IgG (1:20,000, GE Healthcare), horseradish peroxidase-linked anti-rabbit IgG (1:20,000, GE Healthcare), and horseradish peroxidase-linked whole antibody anti-mouse IgG (1:20,000, GE Healthcare).
5
Western Blot Analysis of DNA Repair Proteins
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Western blot analysis was performed as previously described26 , 27 , 28 , 29 with the following antibodies: mouse anti‐PARG (D8B10) monoclonal antibody (1:500; MABS61, Sigma‐Aldrich), rabbit anti‐PARP polyclonal antibody (1:1000; #9542, Cell Signaling Technologies, Danvers, MA, United States), mouse anti‐BRCA1(D‐9) monoclonal antibody (1:200; sc‐6954, Santa Cruz Biotechnology, Dallas, MA, United States), mouse anti‐poly(ADP‐ribose) monoclonal antibody (10H) (1:500; ALX‐804‐220, Enzo Life Sciences, Farmingdale, NY, United States), rabbit anti‐GAPDH antibody (1:20000; 2275‐PC‐100, Trevigen, Gaithersburg, MD, United States), horseradish peroxidase‐linked anti‐rabbit IgG (1:20000, GE Healthcare, Marlborough, MA, United States), and horseradish peroxidase‐linked whole antibody anti‐mouse IgG (1:20000, GE Healthcare).
6
Western Blot Analysis of Thymidylate Synthase
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Western blot analysis was performed as previously described [29 (link),32 (link),33 (link),34 (link)]. The antibodies used were rabbit anti-thymidylate synthase (D5B3) monoclonal antibody (9045S, 1:1000, Cell Signaling Technologies, Danvers, MA, USA), mouse anti-DPYD (A-5) monoclonal antibody (sc-376712, 1:1000, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-GAPDH antibody (2275-PC-100, 1:20,000, Trevigen, Gaithersburg, MD, USA), mouse anti-beta-actin monoclonal antibody (A19178-200UL, 1:20,000, Sigma-Aldrich), horseradish peroxidase-linked anti-rabbit IgG (1:20,000, GE Healthcare, Pittsburgh, PA, USA), and horseradish peroxidase-linked whole antibody anti-mouse IgG (1:20,000, GE Healthcare).
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