16 16 dimethyl pge2

Manufactured by Bio-Techne

Sourced in United Kingdom

16,16-dimethyl-PGE2 is a synthetic prostaglandin analogue. It is a chemical compound used in research and laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

Forskolin, by Bio-Techne (2 mentions) Ah6809, by Bio-Techne (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) L161982, by Bio-Techne (1 mentions) Ly29004, by Cell Signaling Technology (1 mentions) Niflumic acid, by Cayman Chemical (1 mentions) Celecoxib, by Merck Group (1 mentions) Xav939, by Bio-Techne (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) L 161 982, by Merck Group (1 mentions) Mouse anti brdu antibody, by Merck Group (1 mentions) Dobutamine hydrochloride, by Bio-Techne (1 mentions) Zombie aqua viability dye, by BioLegend (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Hil 2, by Thermo Fisher Scientific (1 mentions) Ova peptide, by GenScript (1 mentions) Cgs 21680 hydrochloride, by Bio-Techne (1 mentions)

3 protocols using 16 16 dimethyl pge2

Pharmacological Inhibitors for Epac1 and Epac2

Check if the same lab product or an alternative is used in the 5 most similar protocols

16,16-dimethyl-PGE₂, L161,982, AH6809 and Forskolin were from Tocris Bioscience (Bristol, UK). Ly29004 was from Cell Signaling (Beverly, MA). The pharmacological inhibitor for Epac1, CE3F4 was developed by F. Lezoualc'h [37 (link)]. The pharmacological inhibitor for Epac2, ESI-05 was developed by X. Cheng [38 (link)]. TRIzol® was from Thermo Fisher Scientific (Waltham, MA). All other chemicals were of analytical grade.

Jansen S.R., Poppinga W.J., de Jager W., Lezoualc'h F., Cheng X., Wieland T., Yarwood S.J., Gosens R, & Schmidt M. (2016). Epac1 links prostaglandin E2 to β-catenin-dependent transcription during epithelial-to-mesenchymal transition. Oncotarget, 7(29), 46354-46370.

+ Open protocol

+ Expand

Analyzing Cell Proliferation Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

BrdU, mouse anti-BrdU antibody, PI, L-161,982 and celecoxib were purchased from Sigma-Aldrich. 16,16-dimethyl-PGE₂, XAV939, AH6809 and forskolin were from Tocris Bioscience (Bristol, UK). Niflumic acid was from Cayman Chemical (Ann Arbor, MI, USA). All other chemicals were of analytical grade.

Jansen S.R., Holman R., Hedemann I., Frankes E., Elzinga C.R., Timens W., Gosens R., de Bont E.S, & Schmidt M. (2014). Prostaglandin E2 promotes MYCN non-amplified neuroblastoma cell survival via β-catenin stabilization. Journal of Cellular and Molecular Medicine, 19(1), 210-226.

+ Open protocol

+ Expand

OT-1 T Cell Cytotoxicity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes were isolated from OT-1 mice (4–6 weeks old) and activated with 100 nM OVA peptide (257–264; GenScript) and 50 U ml^–1 h IL-2 (PeproTech) for 48 h. After 48 h, fresh medium with 50 U ml^–1 IL-2 was added to the culture and incubated for an additional 24 h. Cells were then collected, replated and expanded at 1 × 10⁶ cells per ml.
For the specific cytotoxicity assay, tumor cells (target) were plated at 50,000 cells per well in a 24-well plate. OT-1 T cells (effector) were then added at a 1:5 target:effector ratio. Where indicated, GPCR agonists were added at the following concentrations: 1 μM 16,16-dimethyl PGE₂ (Tocris), 5 μM dobutamine hydrochloride (Tocris) and 5 μM CGS-21680 hydrochloride (Tocris). The coculture was left for 36 h, and cell viability was assessed by flow cytometric staining with Zombie Aqua viability dye (BioLegend).

, & Phillippy B.Q. (1998). Identification of Inositol 1,3,4-Trisphosphate 5-Kinase and Inositol 1,3,4,5-Tetrakisphosphate 6-Kinase in Immature Soybean Seeds. Plant Physiology, 116(1), 291-297.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!