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Grace s supplemented medium

Manufactured by Thermo Fisher Scientific
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Grace's Supplemented Medium is a cell culture medium developed for the propagation of insect cells. It is a complete and defined medium that supports the growth of a variety of insect cell lines.

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Lab products found in correlation

Cmrl 1066, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions)

Close spelling variants of this product

Grace’s medium Grace medium

2 protocols using grace s supplemented medium

1

HER2/neu Expressing Mammary Tumor Cell Line

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The HER2/neu expressing NBT1 mammary tumor cell line was derived by serial cell culture of tumor cells obtained from a spontaneous breast tumor that arose in a FVB/neuT mouse transgenic for a HER2/neu receptor that is constitutively activated by a point mutation in the transmembrane region. NBT1 was maintained in media composed of CMRL-1066 (Invitrogen, Carlsbad, CA) supplemented with 10% FBS, 2mM L-glutamine, 50 IU/mL penicillin/streptomycin, and 4μM Dexamethasone. Splenic cell cultures used in immune analyses were carried out using a supplemented RPMI-1640 media as described 21 (link). The Sf9 and Sf21 cell lines (Invitrogen) were maintained in Grace’s Supplemented Medium (Invitrogen) supplemented with 10% FBS and 50 IU/ml penicillin/streptomycin. HER2/neu Sf21 cell lysates used for in vitro restimulation of CTL assay described below were prepared by resuspending cells in RPMI medium with Protease Inhibitor Cocktail (Sigma-Aldrich), lysing via 4 freeze/thaw cycles, sonicating for 2 minutes, and centrifugation for 15 minutes at 13,500 rcf.
de Vries C.R., Monken C.E, & Lattime E.C. (2015). The Addition of Recombinant Vaccinia HER2/neu to Oncolytic Vaccinia-GMCSF Given into the Tumor Microenvironment Overcomes MDSC-Mediated Immune Escape and Systemic Anergy. Cancer gene therapy, 22(3), 154-162.
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2

HER2/neu Expressing Mammary Tumor Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HER2/neu expressing NBT1 mammary tumor cell line was derived by serial cell culture of tumor cells obtained from a spontaneous breast tumor that arose in a FVB/neuT mouse transgenic for a HER2/neu receptor that is constitutively activated by a point mutation in the transmembrane region. NBT1 was maintained in media composed of CMRL-1066 (Invitrogen, Carlsbad, CA) supplemented with 10% FBS, 2mM L-glutamine, 50 IU/mL penicillin/streptomycin, and 4μM Dexamethasone. Splenic cell cultures used in immune analyses were carried out using a supplemented RPMI-1640 media as described 21 (link). The Sf9 and Sf21 cell lines (Invitrogen) were maintained in Grace’s Supplemented Medium (Invitrogen) supplemented with 10% FBS and 50 IU/ml penicillin/streptomycin. HER2/neu Sf21 cell lysates used for in vitro restimulation of CTL assay described below were prepared by resuspending cells in RPMI medium with Protease Inhibitor Cocktail (Sigma-Aldrich), lysing via 4 freeze/thaw cycles, sonicating for 2 minutes, and centrifugation for 15 minutes at 13,500 rcf.
de Vries C.R., Monken C.E, & Lattime E.C. (2015). The Addition of Recombinant Vaccinia HER2/neu to Oncolytic Vaccinia-GMCSF Given into the Tumor Microenvironment Overcomes MDSC-Mediated Immune Escape and Systemic Anergy. Cancer gene therapy, 22(3), 154-162.
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