6 his

AHSA1, N-cadherin, 6-his, vimentin, HSP90, MEK1/2, E-cadherin, and CALD1 antibodies were obtained from Proteintech (Wuhan, China). ERK1/2, Phospho-MEK1/2, and Phospho-ERK1/2 antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Ki67 antibody was obtained from Abcam (Cambridge, MA, USA). Phospho-CALD1 antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). β-tubulin antibody was obtained from Bioworld Technology (Bloomington, MN, USA).

T0901317, bexarotene, ABC294640, SLM6031434, and FTY720 were purchased from Cayman Chemical (Ann Arbor, MI). Trichostatin A (#HY-15144) was purchased from MedChemExpress (Monmouth Junction, NJ). Synthetic Aβ_1-42 peptide (Peptide Institute, Osaka, Japan) was solubilized in hexafluoro-2-propanol at a concentration of 1 mg/ml to prevent self-aggregation. For immunoblotting and immunostaining, antibodies against human Aβ N terminal (clone 82E1; Immuno-Biological Laboratories, Gunma, Japan), ATP binding cassette transporter A1 (ABCA1) (clone HJ1; Abnova, Taipei, Taiwan), ApoE (#AB947; Merck Millipore, Burlington, MA), α-tubulin (clone 10G10; Fujifilm Wako, Osaka, Japan), β-actin (#5125; Cell Signaling Technology, Danvers, MA), GAPDH (#2118; Cell Signaling Technology), glial fibrillary acidic protein (GFAP) (#GTX108711; GeneTex, Irvine, CA), Lamin A/C (#10298-1-AP; Proteintech, Rosemont, IL), LXRα/β (#sc-377260; Santa Cruz Biotechnology, Dallas, TX), nuclear factor-κB (NF-κB) p65 (#8242; Cell Signaling Technology), RXRα (#sc-515929; Santa Cruz Biotechnology), SphK2 (#17096-1-AP; Proteintech), and 6His (#66005-1-Ig; Proteintech) were purchased from the indicated vendors.

The protein was extracted using RIPA buffer from Service-Bio in Wuhan, China, which also contains phosphatase and protease inhibitors, and the protein concentration was assessed using a BCA kit from the same company. The ensuing steps were the same as those in our earlier investigation. The incubated antibodies include LAD1 (16136-1-AP, Proteintech, China, 1:1000), GAPDH (60004-1-Ig, Proteintech, China, 1:1000), Vimentin (10366-1-AP, Proteintech, China, 1:1000), MAEA (28363-1-AP, Proteintech, China, 1:1000), HA-tag (66006-2-Ig, Proteintech, China, 1:1000), Flag (F1804, Sigma, China, 1:1000), 6×His (10001-0-AP, Proteintech, China, 1:1000), and Myc-tag (60003-2-Ig, Proteintech, China, 1:1000).