The HPLC experiments were performed on: Agilent 1200 series chromatographic system encompassed with a quaternary pump, a micro vacuum degasser, a thermostatted column compartment and a variable wavelength UV–VIS detector. In addition, Agilent 1200 series autosampler was used for sample injection. Agilent ChemStation software, version A.10.01 was utilized to collect and process data. Separation was performed on Agilent Zorbax SB-C18 (150 × 4.6 mm, 5 μm) column which is manufactured by Agilent Technologies (Polo Alto, CA, USA). To adjust the pH, a “Jenway 3505” pH-meter (Jenway, UK) was used.
Agilent 1200 series autosampler
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 1200 series autosampler is a liquid handling device used in analytical chemistry applications. It automatically injects samples into a chromatography system for analysis. The autosampler's core function is to accurately and precisely introduce samples into the analytical instrument.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 1200 series controller, by Agilent Technologies (3 mentions)
Sevencompact ph ion s220, by Thermo Fisher Scientific (3 mentions)
Hereus biofuge primo r, by Thermo Fisher Scientific (3 mentions)
Agilent 1200 series dad detector, by Agilent Technologies (3 mentions)
Agilent 1200 series binary pump, by Agilent Technologies (3 mentions)
Chemstation software, by Agilent Technologies (3 mentions)
Agilent 1200 series chromatographic system, by Agilent Technologies (1 mentions)
Agilent 1200 series diode array detector, by Agilent Technologies (1 mentions)
Agilent 1200 series vacuum degasser, by Agilent Technologies (1 mentions)
Agilent 1200 series, by Agilent Technologies (1 mentions)
Duran bottles, by Thermo Fisher Scientific (1 mentions)
Polysep gfc p 4000 column, by Phenomenex (1 mentions)
Polysep gfc p guard column, by Phenomenex (1 mentions)
Optilab dsp interferometric refractometer, by Wyatt Technology (1 mentions)
6 protocols using agilent 1200 series autosampler
1
HPLC Analysis of Chemical Compounds
2
HPLC Analysis of Compounds Using Agilent 1200 Series
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The HPLC system used in the present study was Agilent 1200 Series equipped with Quaternary Pump, Agilent 1200 Series Vacuum Degasser, Agilent 1200 Series Autosampler and Agilent 1200 Series Diode Array Detector Purchased from Agilent Technologies (Hewlett-Packard Strasse Waldbronn, Germany). Chromatographic separation of the compounds was performed on a C18 analytical column (Techsphere 5ODS, 25 cm × 4.6 mm ID; HPLC Technology, Macclesfield, Cheshire, UK). Data acquisition and processing were accomplished with LC Chemstation software (Agilent Technologies). Adwa pH meter (AD1020 pH/mV/ISE/Temperature, Hungary) was used for the determination of the sample pH and A 800 model centrifuge, China, was used to speed up the phase separation. An electronic balance (Adam Equipment Company, UK) was utilized for weighing the different chemicals involved in the experiments. For the measurement of total dissolved solids (TDS) and electrical conductivity, conductivity meter (Postfach 24 80, Germany) was used.
3
Partition Experiments for Dissolution Analysis
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Partition experiments were run in triplicate at 37 °C, using 50 mL Corning ® PP selfstanding centrifuge tubes, the USP 2 paddle apparatus (Agilent 708-DS Dissolution Apparatus) or 100 mL glass DURAN ™ bottles (Fischer). All samples were analysed in an HPLC system consisting of an Agilent 1200 series binary pump (G1312A), an Agilent 1200 series DAD detector (G1315D), an Agilent 1200 series autosampler (G1329A), an Agilent 1200 series controller (G1316A) and Chemstation software (Agilent Technologies, Santa Clara, United States). A pH meter Mettler-Toledo AG (model SevenCompact pH/Ion S220, Schwerzenbach, Switzerland), a centrifuge Hereus Biofuge Primo R (Thermo Scientific, Hanau, Germany) and a vortex mixer Rotamixer (HTZ, Cheshire, UK) were used.
4
HPLC Analysis of Chemical Compounds
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All samples were analysed in an HPLC system consisting of an Agilent 1200 series binary pump (G1312A), an Agilent 1200 series DAD detector (G1315D), an Agilent 1200 series autosampler (G1329A), an Agilent 1200 series controller (G1316A) with a Chemstation software (Agilent Technologies, Santa Clara, United States).
A pH meter Mettler-Toledo AG (model SevenCompact pH/Ion S220, Schwerzenbach, Switzerland), a centrifuge Hereus Biofuge Primo R (Thermo Scientific, Hanau, Germany) and a vortex mixer Rotamixer (HTZ, Cheshire, UK) were used.
A pH meter Mettler-Toledo AG (model SevenCompact pH/Ion S220, Schwerzenbach, Switzerland), a centrifuge Hereus Biofuge Primo R (Thermo Scientific, Hanau, Germany) and a vortex mixer Rotamixer (HTZ, Cheshire, UK) were used.
5
HPLC Analysis of Compound Mixtures
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All samples were analysed in an HPLC system consisting of an Agilent 1200 series binary pump (G1312A), an Agilent 1200 series DAD detector (G1315D), an Agilent 1200 series autosampler (G1329A), an Agilent 1200 series controller (G1316A) and a Chemstation software (Agilent Technologies, Santa Clara, United States).
A pH meter Mettler-Toledo AG (model SevenCompact pH/Ion S220, Schwerzenbach, Switzerland), a centrifuge Hereus Biofuge Primo R (Thermo Scientific, Hanau, Germany), a vortex mixer Rotamixer (HTZ, Chesire, UK) and, a UV-Vis Thermo Spectronic Helios Gamma spectrophotometer (Thermo Scientic, UK) were used.
A pH meter Mettler-Toledo AG (model SevenCompact pH/Ion S220, Schwerzenbach, Switzerland), a centrifuge Hereus Biofuge Primo R (Thermo Scientific, Hanau, Germany), a vortex mixer Rotamixer (HTZ, Chesire, UK) and, a UV-Vis Thermo Spectronic Helios Gamma spectrophotometer (Thermo Scientic, UK) were used.
6
Molecular Weight Determination of Polymers
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The molecular weights of GCPQ and GCPh were determined as detailed below.
Specific refractive index increments (dη/dc) of the polymers were measured in a mixture of acetate buffer [CH3COONa (0.3 M), CH3COOH (0.2 M), pH 5]: methanol (35: 65) at 37 °C with an Optilab DSP interferometric refractometer (Wyatt Technology Corporation, USA) set at a wavelength of 690 nm. Filtered (0.2 μm) samples (0.1 to 1 mg mL -1 ) were manually injected at a flow rate of 0.3 mL min -1 and the data obtained was processed using DNDC for Windows version 5.90.03 software (Wyatt Technology Corporation, USA).
The molecular weight of GCPQ was measured using Gel Permeation Chromatography -Multi-Angle Laser Light Scattering (GPC -MALLS). Measurements were performed using a DAWN EOS MALLS detector (λ = 690 nm), Optilab DSP interferometric refractometer (λ = 690 nm) and a Quasi-Elastic Light Scattering (QELS) detector (Wyatt Technology Corporation, U.S.A.). Filtered samples of GCPQ (0.2 μm, 200 μL)
were injected into a POLYSEP-GFC-P 4000 column (300 × 7.8 mm, Phenomenex, U.K.) fitted with a POLYSEP-GFC-P guard column (35 × 7.8 mm, Phenomenex, U.K.) using an Agilent 1200 series auto-sampler (Agilent Technologies, USA.) at a loading concentration of 5 mg mL -1 with a flow rate of 0.7 mL min -1 . The data were analysed using ASTRA for Windows version 4.90.08 software.
Specific refractive index increments (dη/dc) of the polymers were measured in a mixture of acetate buffer [CH3COONa (0.3 M), CH3COOH (0.2 M), pH 5]: methanol (35: 65) at 37 °C with an Optilab DSP interferometric refractometer (Wyatt Technology Corporation, USA) set at a wavelength of 690 nm. Filtered (0.2 μm) samples (0.1 to 1 mg mL -1 ) were manually injected at a flow rate of 0.3 mL min -1 and the data obtained was processed using DNDC for Windows version 5.90.03 software (Wyatt Technology Corporation, USA).
The molecular weight of GCPQ was measured using Gel Permeation Chromatography -Multi-Angle Laser Light Scattering (GPC -MALLS). Measurements were performed using a DAWN EOS MALLS detector (λ = 690 nm), Optilab DSP interferometric refractometer (λ = 690 nm) and a Quasi-Elastic Light Scattering (QELS) detector (Wyatt Technology Corporation, U.S.A.). Filtered samples of GCPQ (0.2 μm, 200 μL)
were injected into a POLYSEP-GFC-P 4000 column (300 × 7.8 mm, Phenomenex, U.K.) fitted with a POLYSEP-GFC-P guard column (35 × 7.8 mm, Phenomenex, U.K.) using an Agilent 1200 series auto-sampler (Agilent Technologies, USA.) at a loading concentration of 5 mg mL -1 with a flow rate of 0.7 mL min -1 . The data were analysed using ASTRA for Windows version 4.90.08 software.
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