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Ab100540

Manufactured by Abcam
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Sourced in United Kingdom

Ab100540 is a recombinant monoclonal antibody that specifically recognizes Human Transferrin Receptor. The antibody is produced in mammalian cells and purified using protein A affinity chromatography.

Automatically generated - may contain errors

Lab products found in correlation

Ab100624, by Abcam (1 mentions) Ab100663, by Abcam (1 mentions) Exl800, by Agilent Technologies (1 mentions)

2 protocols using ab100540

1

IGFBP-2, PECAM-1, and NECTIN-2 Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum levels of IGFBP-2 (ab100540, Abcam, Cambridge, UK, lower detection limit 20 pg/mL), PECAM-1 (ELH-PECAM-1, RayBiotech, Peachtree Corners, GA, USA), lower detection limit 15 pg/mL), and NECTIN-2 (ELH-NEC2, RayBiotech, lower detection limit 0.12 ng/mL) were measured using ELISA with intra- and inter-assay coefficients of variation of 10% and <12%, respectively, following the manufacture’s protocols.
Machado F.J., Marta-Enguita J., Gómez S.U., Rodriguez J.A., Páramo-Fernández J.A., Herrera M., Zandio B., Aymerich N., Muñoz R., Bermejo R., Marta-Moreno J., López B., González A., Roncal C, & Orbe J. (2024). Transcriptomic Analysis of Extracellular Vesicles in the Search for Novel Plasma and Thrombus Biomarkers of Ischemic Stroke Etiologies. International Journal of Molecular Sciences, 25(8), 4379.
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2

Quantifying RPE Secreted Factors

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After 3 days of RPE cells incubation, culture media was collected and measured for PDGF-BB (Abcam; ab100624), VEFG (Abcam; ab100663) and IGFBP-2 (Abcam; ab100540) levels using human ELISA kit)according to the manufacturer's protocol. The optical densities were determined within 30 min and recorded with a microplate reader (BioTek, EXL800, Winooski, VT) at 450 nm.
Chang Y.C., Chang W.C., Hung K.H., Yang D.M., Cheng Y.H., Liao Y.W., Woung L.C., Tsai C.Y., Hsu C.C., Lin T.C., Liu J.H., Chiou S.H., Peng C.H, & Chen S.J. (2014). The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Frontiers in Aging Neuroscience, 6, 191.
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