Mono 2 ethylhexyl phthalate mehp

Manufactured by AccuStandard

Sourced in United States

Mono(2-ethylhexyl)phthalate (MEHP) is a chemical compound that is commonly used as a plasticizer in various applications. It is a colorless, odorless liquid with a high boiling point and low solubility in water. MEHP is used to increase the flexibility, durability, and workability of materials, particularly in the production of PVC products. This compound can be utilized in a range of industries, including construction, packaging, and consumer goods manufacturing.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) F 12k nutrient mixture, by Thermo Fisher Scientific (1 mentions) Forskolin, by Merck Group (1 mentions) Heat inactivated fetal bovine serum hi fbs, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) 5 azacytidine 5 ac, by Merck Group (1 mentions) Diisononyl phthalate, by Merck Group (1 mentions) Di 2 ethylhexyl phthalate, by Merck Group (1 mentions) Wy14643, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Monobenzyl phthalate, by AccuStandard (1 mentions)

5 protocols using mono 2 ethylhexyl phthalate mehp

Phthalate Exposure Protocols

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All chemicals used in these experiments were of the highest purity grade available. Mono(2-ethylhexyl)phthalate (MEHP) was purchased from AccuStandard (New Haven, CT, USA). Dimethylsulfoxide (DMSO) was purchased from Fisher Scientific (Pittsburg, PA, USA). A stock solution of 2 mg/mL was prepared by diluting neat MEHP in DMSO. Solutions were stored in amber vials at −20 °C and vortexed before each use. All procedures involving the handling of MEHP were performed utilizing proper PPE and standard laboratory safety precautions. All other chemicals used in this study were purchased from Fisher Scientific.

Jacobs H.M., Sant K.E., Basnet A., Williams L.M., Moss J.B, & Timme-Laragy A.R. (2017). Embryonic Exposure to Mono(2-ethylhexyl) Phthalate (MEHP) Disrupts Pancreatic Organogenesis in Zebrafish (Danio rerio). Chemosphere, 195, 498-507.

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Oxidative Stress Response Bioassay

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Monochlorobimane (Catalog #M13813MP) was purchased from Molecular Probes (Eugene, OR, USA). Dimethyl Sulfoxide (DMSO; Catalog #BP231-1) was purchased from Fisher Scientific (Pittsburgh, PA, USA). Tert-Butyl hydroperoxide (tBOOH; Catalog #A13926AP) and N-acetyl-L-cysteine (NAC; Catalog #A1540914) were purchased from Alfa Aesar (Ward Hill, MA, USA). Ethacrynic Acid (ETA; Catalog #BMLEI1280001) and L-Buthionine-(S,R)-sulfoximine (BSO; Catalog #BML-FR117-0500) were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Potassium perfluorooctane sulfonate (PFOS; Catalog #33829) was purchased from Millipore-Sigma (Burlington, MA, USA). Mono(2-ethylhexyl) phthalate (MEHP; Catalog #ALR-138S–CN) was purchased from AccuStandard (New Haven, CT, USA).

Rastogi A., Clark C.W., Conlin S.M., Brown S.E, & Timme-Laragy A.R. (2019). Mapping glutathione utilization in the developing zebrafish (Danio rerio) embryo. Redox Biology, 26, 101235.

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Culturing Cells with Exosome-Depleted Serum

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Iscove’s Modified Dulbecco’s Medium (IMDM), F12-K Nutrient Mixture Kaighn’s Modification with (+) L Glutamine, Dulbecco’s Modified Eagle Medium (DMEM)/F12 Nutrient Mixture without phenol red, penicillin/streptomycin (P/S), heat-inactivated fetal bovine serum (HI-FBS) and exosome-depleted fetal bovine serum (ED-FBS), which contains over 90% of exosomes depleted, were purchased from Gibco (Grand Island, NY). Phosphate buffered saline (PBS) and 0.25% trypsin-EDTA were from Invitrogen Life Technologies (Carlsbad, CA). Dimethyl sulfoxide (DMSO) was purchased from Tocris Bioscience (Bristol, United Kingdom). Forskolin and 2-mercaptoethanol were purchased from Sigma-Aldrich (St. Louis, MO). Mono-2-ethylhexyl phthalate (MEHP) was purchased from AccuStandard (New Haven, CT).

Harris S.M., Su A.L., Dou J.F., Loch-Caruso R., Elkin E.R., Jaber S, & Bakulski K.M. (2023). Placental Cell Conditioned Media Modifies Hematopoietic Stem Cell Transcriptome In Vitro. bioRxiv.

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Liquid Chromatography-Mass Spectrometry Analysis of DNA Methylation Markers

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5-Azacytidine (5AC, >98%) was obtained from Sigma (Germany). Mono(2-ethylhexyl) phthalate (MEHP, >99%) was purchased from AccuStandard (USA). Dimethylsulfoxide was purchased from Acros Chemicals (Belgium). The standards 5-methyl-2′-deoxycytidine (mC) and 2′-deoxyguanosine (G) and 5-hydroxymethyl-2′-deoxycytidine (hmC) were purchased at MP biomedicals (the Netherlands), Sigma (Germany) and Carbosynth (UK), respectively. The internal standards for LC/MS analysis (2′-deoxyguanosine-¹³C10,¹⁵N5 (G-¹³C10¹⁵N5), 5-methyl-2′-deoxycytidine-d3 (mC-d3) and 5-hydroxymethyl-2′-deoxycytidine-d3 (hmC-d3)) were obtained from Toronto research chemicals (TRC, Canada).

Kamstra J.H., Sales L.B., Aleström P, & Legler J. (2017). Differential DNA methylation at conserved non-genic elements and evidence for transgenerational inheritance following developmental exposure to mono(2-ethylhexyl) phthalate and 5-azacytidine in zebrafish. Epigenetics & Chromatin, 10, 20.

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Chemical Standards for Phthalate and Nuclear Receptor Assays

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General chemicals, di(2-ethylhexyl) phthalate (DEHP, CAS# 117-81-7), 5α-androstan-3α-ol (ANDRO, CAS# 7657-50-3), di-isononyl phthalate (DiNP, CAS# 68515-48-0), dimethyl sulfoxide (DMSO, CAS# 67-68-5), troglitazone (TG, acs-045, CAS# 97322-87-7), and WY14643 were purchased from Sigma-Aldrich. Benzyl butyl phthalate (BBP, CAS# 85-68-7), monobenzyl phthalate (MBzP, CAS# 2528-16-7), mono-(2-ethylhexyl)phthalate (MEHP, CAS# 4376-20-9), monoethyl phthalate (MEP, CAS# 2306-33-4), and monoiso-nonyl phthalate (MiNP, isometrically pure 9 carbon ester, CAS# EDF-014) were purchased from AccuStandard (New Haven, CT). Di(2-propyl heptyl) phthalate (DPHP, CAS# 53306-54-0) was purchased from Toronto Research Chemicals (Ontario, CA), and phthalic acid (PA, CAS# 88-99-3) was purchased from TCI America (Portland, OR). 6-(4-Chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-3,4-dichlorobenzyl) oxime (CITCO, CAS# 338404-52-7) was obtained from BIOMOL Research Laboratories (Plymouth Meeting, PA). TO901317 (TO, CAS# 293754-55-9) was obtained from Cayman Chemical Co. (Ann Arbor, MI). GW501516 (CAS# 50892-23-4) was purchased from Calbiochem. Primers were purchased from Integrated DNA Technologies (Coralville, IA).

Laurenzana E.M., Coslo D.M., Vigilar M.V., Roman A.M, & Omiecinski C.J. (2016). Activation of the Constitutive Androstane Receptor by Monophthalates. Chemical research in toxicology, 29(10), 1651-1661.

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