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2 protocols using β galactosidase

1

Fungal Metabolism in Guizhou Chestnuts

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Chestnuts (Castanea mollissima Blume, Guizhou wild chestnuts) came from Guizhou Guangxiu Ecological Food Co., Ltd., and were produced in Wangmo County, Guizhou Province. Fusarium proliferatum, Penicillium crustosum, and Alternaria alternata were isolated from Wangmo rotten chestnuts and stored in the laboratory (Zhu et al., 2023 (link)). β-Galactosidase, superoxide dismutase, and α-amylase activity detection reagent kits were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). All fungi in this experiment were cultured in potato dextrose agar (PDA) medium (Haibo Biotechnology Co., Ltd., Qingdao, Shandong, China). All other chemicals and reagents were of analytical grade and purchased from Sinopharm Chemical Reageant Co. Ltd. (Suzhou, Jiangsu, China) and were of analytical grade.
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2

Alzheimer's Disease Biomarker Assay

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Pectinase, cellulase, β-galactosidase, dextranase, β-mannase, and standards for maltose (purity ≥98%), maltotriose (purity ≥98%), malttetraose (purity ≥97%), and maltpentaose (purity ≥7%) were products of Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). D-glucose standard (purity ≥98%) was obtained from Sichuan Weikeqi Biotechnology Co., Ltd. (Chengdu, China). Moreover, 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) was purchased from Meryer (Shanghai, China) Biochemical Technology Co., Ltd. (Shanghai, China). Aβ25–35 and dimethylsulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Gibco (Burlington, ON, Canada), Annexin V-APC/PI and Hoechst33342/PI dual staining kits were from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing, China), Dulbecco’s modified Eagles medium (DMEM) cell culture medium was purchased from Procell Life Science &Technology Co., Ltd. (Wuhan, China). Trypsin-EDTA from Hyclone Laboratories (Logan, UT, USA). Detection kits for ROS, SOD, LDH and MDA were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TRIzol reagent and the RevertAid First Strand cDNA Synthesis Kit was purchased from Thermo Fisher (Waltham, MA, USA).
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